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Molecular-Cloning
331.
Gel purification (DNA) -
(reply: 1)
332.
No insert for PCR cloning and restriction enzyme digestion -
(reply: 4)
333.
Cloning and expression problem -
(reply: 5)
334.
How do silicates adhere nucleic acid? -
(reply: 1)
335.
Ligation of PCR fragments -
(reply: 11)
336.
No Colonies after Electroporation of P.aeruginosa -
(reply: 6)
337.
What plasmid sizes do you work with for SDM? -
(reply: 1)
338.
cloning help; high salt level in vector -
(reply: 4)
339.
Help! Plasmid resistant to restriction digest after acquiring Flag tag -
(reply: 3)
340.
problem with gateway cloning: missing part of insert -
(reply: 3)
341.
T7 and M13 primers two band amplification -
(reply: 3)
342.
Type of polymerases compatible for TA cloning -
(reply: 7)
343.
Running undigested plasmids on gel -
(reply: 2)
344.
Molecular Cloning Sambrook -
(reply: 1)
345.
What is the membrane made of in DNA purification kits -
(reply: 3)
346.
Plasmid Vector - CMV enhancer / promoter fluorescent reporter, a positive or neg -
(reply: 3)
347.
Can you engineer a kinetochore in a BAC so that it segregates evenly -
(reply: 3)
348.
Removing Introns from a DNA sequence -
(reply: 1)
349.
transfecting Jurkat with Fugene HD: does not work? -
(reply: 4)
350.
without RE site in PCR product -
(reply: 5)
351.
Will long exposures of DNA to UV (302nm) alter sequencing data? -
(reply: 4)
352.
excision of fragment during cloning -
(reply: 4)
353.
Unusual cloning issue... anyone else?? -
(reply: 4)
354.
Puzzling difficulty cutting out one gene sequence for another -
(reply: 4)
355.
Trying to create deletion mutant. Stuck on last step. -
(reply: 1)
356.
How to avoid RNA contamination in DNA samples:? -
(reply: 1)
357.
Wavy Lines in Gel -
(reply: 2)
358.
Question Relating to LacZ Disruption -
(reply: 4)
359.
Recombinant Plasmid is not being amplified in transformed cells -
(reply: 2)
360.
Site directed mutagenesis -
(reply: 5)
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