|
1231. Nco1 digest problem - (reply: 4)
1232. Frameshift Mutation at the plasmid level - If your PCR product is sequence verified are you good to go? (reply: 5)
1233. ligation - (reply: 1)
1234. pre-prepararation of competent cells - (reply: 3)
1235. different copy # multimers with linker ligation? - (reply: 2)
1236. Help ADD 3 bases to DNA Sequences - (reply: 6)
1237. My battle with BamHI - (reply: 35)
1238. serious problem in cloning - (reply: 2)
1239. What are "Ella-cre" mice? - (reply: 2)
1240. help with pCMV-myc vector - (reply: 2)
1241. Elution amount - (reply: 5)
1242. keeping restriction digested fragments at -20? - (reply: 5)
1243. Need help with pkD46, Pkd13, pCP20 - Need Help (reply: 2)
1244. re-digestion - (reply: 1)
1245. Freezing PCR products prior to ligation - To use a blunt-end vector or an overhang vector? (reply: 2)
1246. Cloning using BamH1 and HindIII - (reply: 8)
1247. frozen competent cells - how to prepare competent cells from frozen bacteria? (reply: 3)
1248. "competentcy" of my method - (reply: 5)
1249. Vector frameshift problem - (reply: 3)
1250. Screening colonies after transformation - (reply: 2)
1251. z competent cells - (reply: 2)
1252. minimum amounts of insert and vector for ligation - to have a successful cloning? (reply: 7)
1253. insert release not as intense as the plasmid - is it always like that? (reply: 3)
1254. partial DNA digestion formula - Linear DNA partial digestion (reply: 1)
1255. plasmid extraction kit wash buffer solution - (reply: 3)
1256. molecular "terminology" - (reply: 1)
1257. NdeI digestion - Share your experience (reply: 3)
1258. Multiple bands after amping insert - (reply: 2)
1259. Good tool for sequence validation? - For Windows (reply: 2)
1260. problem in plasmid isolation - unable to detect false positives in colony pcr (reply: 5)
1232. Frameshift Mutation at the plasmid level - If your PCR product is sequence verified are you good to go? (reply: 5)
1233. ligation - (reply: 1)
1234. pre-prepararation of competent cells - (reply: 3)
1235. different copy # multimers with linker ligation? - (reply: 2)
1236. Help ADD 3 bases to DNA Sequences - (reply: 6)
1237. My battle with BamHI - (reply: 35)
1238. serious problem in cloning - (reply: 2)
1239. What are "Ella-cre" mice? - (reply: 2)
1240. help with pCMV-myc vector - (reply: 2)
1241. Elution amount - (reply: 5)
1242. keeping restriction digested fragments at -20? - (reply: 5)
1243. Need help with pkD46, Pkd13, pCP20 - Need Help (reply: 2)
1244. re-digestion - (reply: 1)
1245. Freezing PCR products prior to ligation - To use a blunt-end vector or an overhang vector? (reply: 2)
1246. Cloning using BamH1 and HindIII - (reply: 8)
1247. frozen competent cells - how to prepare competent cells from frozen bacteria? (reply: 3)
1248. "competentcy" of my method - (reply: 5)
1249. Vector frameshift problem - (reply: 3)
1250. Screening colonies after transformation - (reply: 2)
1251. z competent cells - (reply: 2)
1252. minimum amounts of insert and vector for ligation - to have a successful cloning? (reply: 7)
1253. insert release not as intense as the plasmid - is it always like that? (reply: 3)
1254. partial DNA digestion formula - Linear DNA partial digestion (reply: 1)
1255. plasmid extraction kit wash buffer solution - (reply: 3)
1256. molecular "terminology" - (reply: 1)
1257. NdeI digestion - Share your experience (reply: 3)
1258. Multiple bands after amping insert - (reply: 2)
1259. Good tool for sequence validation? - For Windows (reply: 2)
1260. problem in plasmid isolation - unable to detect false positives in colony pcr (reply: 5)