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31. Using pB2GW7,0 - (reply: 2)
32. propagating commercial chemically competent cells - (reply: 2)
33. Plasmid purification for Gibson Assembly - (reply: 2)
34. Unexpected band in BL21 (DE3) plasmid isolation - (reply: 1)
35. TA cloning..Urgent Help!! - (reply: 4)
36. Can someone please explain this to me? - (reply: 2)
37. Problem with polyA retrotranscription: I have truncated products - (reply: 1)
38. TetA as a counter-selection marker - (reply: 1)
39. Wrong or No fragment in Directional Cloning Vector - (reply: 8)
40. pET32b+ vector issue - (reply: 1)
41. Subcloning issue - (reply: 2)
42. Splicing DNA fragments - (reply: 2)
43. Need to add 12 bp at the beginning of the sequence - (reply: 4)
44. Cloning issue with bacteria growing in primary media but not in secondary - (reply: 3)
45. Problem with possible leaky expression in cloning - (reply: 4)
46. Luciferase assay cloning - (reply: 2)
47. Selecting bacteria with Puromycin - (reply: 1)
48. After Gibson assembly or T4-Ligation obtained colonies do not grow anymore - (reply: 1)
49. How stable is double digested plasmid - (reply: 7)
50. Problems with ligation - (reply: 2)
51. Problem with restriction digestion of cloned PCR product - (reply: 5)
52. SLIC Cloning Failure - (reply: 2)
53. Failed Gibson transformation - (reply: 7)
54. how to check for intron-exon junctions - (reply: 2)
55. Lower Plasmid Yields - (reply: 5)
56. Cleaning loading dye for PCR sequencing - (reply: 1)
57. Using SDS for DNA electrophoresis - (reply: 3)
58. Bacterial knockout by double crossover - (reply: 4)
59. Polymerase mixture for blunt ended fragments - (reply: 2)
60. how to design PCR primer with a tag region which use for In-frame deletion gene? - (reply: 10)
32. propagating commercial chemically competent cells - (reply: 2)
33. Plasmid purification for Gibson Assembly - (reply: 2)
34. Unexpected band in BL21 (DE3) plasmid isolation - (reply: 1)
35. TA cloning..Urgent Help!! - (reply: 4)
36. Can someone please explain this to me? - (reply: 2)
37. Problem with polyA retrotranscription: I have truncated products - (reply: 1)
38. TetA as a counter-selection marker - (reply: 1)
39. Wrong or No fragment in Directional Cloning Vector - (reply: 8)
40. pET32b+ vector issue - (reply: 1)
41. Subcloning issue - (reply: 2)
42. Splicing DNA fragments - (reply: 2)
43. Need to add 12 bp at the beginning of the sequence - (reply: 4)
44. Cloning issue with bacteria growing in primary media but not in secondary - (reply: 3)
45. Problem with possible leaky expression in cloning - (reply: 4)
46. Luciferase assay cloning - (reply: 2)
47. Selecting bacteria with Puromycin - (reply: 1)
48. After Gibson assembly or T4-Ligation obtained colonies do not grow anymore - (reply: 1)
49. How stable is double digested plasmid - (reply: 7)
50. Problems with ligation - (reply: 2)
51. Problem with restriction digestion of cloned PCR product - (reply: 5)
52. SLIC Cloning Failure - (reply: 2)
53. Failed Gibson transformation - (reply: 7)
54. how to check for intron-exon junctions - (reply: 2)
55. Lower Plasmid Yields - (reply: 5)
56. Cleaning loading dye for PCR sequencing - (reply: 1)
57. Using SDS for DNA electrophoresis - (reply: 3)
58. Bacterial knockout by double crossover - (reply: 4)
59. Polymerase mixture for blunt ended fragments - (reply: 2)
60. how to design PCR primer with a tag region which use for In-frame deletion gene? - (reply: 10)