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1381. Sequence management software (for Mac) - (reply: 5)
1382. ligation for 24 hrs in 4*C? - (reply: 3)
1383. Having problem establishing stable cell line...... - (reply: 5)
1384. Best method for Mutagenesis? - multisite mutagenesis (reply: 2)
1385. Contruction of Expression Construct - (reply: 1)
1386. problem with subcloning, please help! - (reply: 8)
1387. Doing mutagenesi for large plasmid or articificial chromosome - (reply: 1)
1388. Designing a Plasmid for p53 expression - I must choose between 3 given plasmids the best to express p53. (reply: 1)
1389. Using DNA in gel extract kept at -20ºC - (reply: 2)
1390. Help! Plasmid disappearing after mini-prep. - A260 shows good quality DNA, but nothing appears on gel. (reply: 3)
1391. Help!!! - Unsure of problem (reply: 2)
1392. HELP! Can't double digest pcDNA3.1 vector - (reply: 6)
1393. Help complementing my mutant in trans - help me find a plasmid (reply: 1)
1394. Site directed mutagenesis - Site directed mutagenesis (reply: 1)
1395. DNA yield estimation - (reply: 1)
1396. Vector NTI from Invitrogen - (reply: 4)
1397. gfp-tagged protein not expressing- help! - (reply: 3)
1398. anyone working currently on gateway cloning???? - (reply: 1)
1399. Dpn1 enzyme - (reply: 2)
1400. mutagenesis expert come in~ - (reply: 11)
1401. Subcloning - (reply: 3)
1402. Sequencing different colonies - (reply: 5)
1403. detection of restriction? - (reply: 1)
1404. problem in pcr and purification - (reply: 3)
1405. bacterial stock preparation - (reply: 3)
1406. Problem with site directed mutagenesis - (reply: 7)
1407. ds-oligos cloning problems - 90bp oligo and 12kb plasmid (reply: 2)
1408. Forgot to add X-gal/IPTG - (reply: 8)
1409. after re-isolation of vector, get single band - (reply: 4)
1410. Subcloning into pMIR-REPORT-luc - (reply: 7)
1382. ligation for 24 hrs in 4*C? - (reply: 3)
1383. Having problem establishing stable cell line...... - (reply: 5)
1384. Best method for Mutagenesis? - multisite mutagenesis (reply: 2)
1385. Contruction of Expression Construct - (reply: 1)
1386. problem with subcloning, please help! - (reply: 8)
1387. Doing mutagenesi for large plasmid or articificial chromosome - (reply: 1)
1388. Designing a Plasmid for p53 expression - I must choose between 3 given plasmids the best to express p53. (reply: 1)
1389. Using DNA in gel extract kept at -20ºC - (reply: 2)
1390. Help! Plasmid disappearing after mini-prep. - A260 shows good quality DNA, but nothing appears on gel. (reply: 3)
1391. Help!!! - Unsure of problem (reply: 2)
1392. HELP! Can't double digest pcDNA3.1 vector - (reply: 6)
1393. Help complementing my mutant in trans - help me find a plasmid (reply: 1)
1394. Site directed mutagenesis - Site directed mutagenesis (reply: 1)
1395. DNA yield estimation - (reply: 1)
1396. Vector NTI from Invitrogen - (reply: 4)
1397. gfp-tagged protein not expressing- help! - (reply: 3)
1398. anyone working currently on gateway cloning???? - (reply: 1)
1399. Dpn1 enzyme - (reply: 2)
1400. mutagenesis expert come in~ - (reply: 11)
1401. Subcloning - (reply: 3)
1402. Sequencing different colonies - (reply: 5)
1403. detection of restriction? - (reply: 1)
1404. problem in pcr and purification - (reply: 3)
1405. bacterial stock preparation - (reply: 3)
1406. Problem with site directed mutagenesis - (reply: 7)
1407. ds-oligos cloning problems - 90bp oligo and 12kb plasmid (reply: 2)
1408. Forgot to add X-gal/IPTG - (reply: 8)
1409. after re-isolation of vector, get single band - (reply: 4)
1410. Subcloning into pMIR-REPORT-luc - (reply: 7)