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451. Problems regarding point mutations.... - (reply: 4)
452. Question regarding yeast strain with ura3-52 mutation - (reply: 1)
453. pkp 59 vector - (reply: 2)
454. Vector insert ratio in cloning - (reply: 3)
455. polymerase to use for cloning - (reply: 4)
456. Oligo insert cloning - (reply: 3)
457. Poor expression in my cloned vector (pMSCV-PIG) - (reply: 1)
458. ligating blunt ends of a linearized plasmid, is kinase necessary? - (reply: 1)
459. How to select competent E. coli cells? - (reply: 7)
460. 10,000 bp size band showing up in gel of plasmid extraction - (reply: 1)
461. cDNA amplification problem - (reply: 4)
462. Ligation: two basic questions - (reply: 1)
463. Allelic Exchange in Pseudomonas aeruginosa - (reply: 1)
464. Unexpected base after deletion mutagenesis - (reply: 3)
465. Confirmation of ligation - (reply: 7)
466. 5'end of pcr product was degraded after ligation and transformation - (reply: 5)
467. Ligation of large ammount of insert (no transformation) - (reply: 7)
468. PCR and restriction enzyme digestion - (reply: 3)
469. Probelm with double digestion - (reply: 1)
470. Cloning not working, all steps checked, clueless - (reply: 1)
471. Problems with designing an experiment in identifying mutations of particular gen - (reply: 5)
472. recombinant protein form - (reply: 2)
473. restricted PCR plasmid runs slower - (reply: 2)
474. Cloning problem, sticky - blunt ends ligation fail . - (reply: 6)
475. High frequency of vector religation - (reply: 10)
476. Cloned but not expressing! - (reply: 8)
477. Is it possible to induce signalling pathways activity in Hela cells using multip - (reply: 1)
478. DNA ligation and trasformation - (reply: 6)
479. Stuck from last august! need help urgently - cloning and pcr ! - (reply: 7)
480. Storing NovaBlue Transformation Reactions in SOC - (reply: 1)
452. Question regarding yeast strain with ura3-52 mutation - (reply: 1)
453. pkp 59 vector - (reply: 2)
454. Vector insert ratio in cloning - (reply: 3)
455. polymerase to use for cloning - (reply: 4)
456. Oligo insert cloning - (reply: 3)
457. Poor expression in my cloned vector (pMSCV-PIG) - (reply: 1)
458. ligating blunt ends of a linearized plasmid, is kinase necessary? - (reply: 1)
459. How to select competent E. coli cells? - (reply: 7)
460. 10,000 bp size band showing up in gel of plasmid extraction - (reply: 1)
461. cDNA amplification problem - (reply: 4)
462. Ligation: two basic questions - (reply: 1)
463. Allelic Exchange in Pseudomonas aeruginosa - (reply: 1)
464. Unexpected base after deletion mutagenesis - (reply: 3)
465. Confirmation of ligation - (reply: 7)
466. 5'end of pcr product was degraded after ligation and transformation - (reply: 5)
467. Ligation of large ammount of insert (no transformation) - (reply: 7)
468. PCR and restriction enzyme digestion - (reply: 3)
469. Probelm with double digestion - (reply: 1)
470. Cloning not working, all steps checked, clueless - (reply: 1)
471. Problems with designing an experiment in identifying mutations of particular gen - (reply: 5)
472. recombinant protein form - (reply: 2)
473. restricted PCR plasmid runs slower - (reply: 2)
474. Cloning problem, sticky - blunt ends ligation fail . - (reply: 6)
475. High frequency of vector religation - (reply: 10)
476. Cloned but not expressing! - (reply: 8)
477. Is it possible to induce signalling pathways activity in Hela cells using multip - (reply: 1)
478. DNA ligation and trasformation - (reply: 6)
479. Stuck from last august! need help urgently - cloning and pcr ! - (reply: 7)
480. Storing NovaBlue Transformation Reactions in SOC - (reply: 1)