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1261. Silencing of the first cistron - (reply: 1)
1262. To clean or not to clean.....? - PCR product clean up prior to restriction enzyme digestion (reply: 3)
1263. Huge difference in Tm of my For and Rev primers = no PCR product?!? - (reply: 11)
1264. Stable Mammalian Expression with Retrovirus - (reply: 2)
1265. Neon Transfection System - (reply: 2)
1266. help - (reply: 5)
1267. why they use isopropanal for precipitate DNA in midiprep - (reply: 5)
1268. Molecular Cloning - Cloning of PAPD PCR products (reply: 1)
1269. problems with qiagen gel extraction kit - (reply: 7)
1270. restriction enzyme flanking sequence preferance - (reply: 2)
1271. pCDH Lentivector has 500 bp insert - (reply: 5)
1272. 5' RACE for very GC-rich gene - (reply: 2)
1273. Phosphorylated primers - (reply: 5)
1274. Correct orientation of the insert - (reply: 7)
1275. screening of positive clones - colony PCR /Plasmid isolation and Restriction digestion (reply: 5)
1276. PIR 1 Competent Cells - Poor Transformation Efficiency (reply: 1)
1277. Cloning advice needed - (reply: 4)
1278. pcr amolification with long pcr enzyme mix for cloning - (reply: 1)
1279. Colony Screeing without using PCR - (reply: 8)
1280. blue white screening - (reply: 3)
1281. phosphorylate dprimers for pfx do not work - (reply: 3)
1282. help - (reply: 1)
1283. problem with yeast transformation - (reply: 2)
1284. ta cloning of pfu amplified fragment - (reply: 9)
1285. how to express the gene in opposite transcriptional direction - (reply: 3)
1286. Water for electrocompetent cells - (reply: 2)
1287. AAARGHHH, cloning driving me insane, HELP PLEASE! - UPDATE on same 5' and 3' ends (reply: 2)
1288. Bacterial growth in agar plate with ampicillin - (reply: 4)
1289. problem in ligation and cloning - (reply: 1)
1290. Electrophoresis gel boxes and geldoc system - (reply: 2)
1262. To clean or not to clean.....? - PCR product clean up prior to restriction enzyme digestion (reply: 3)
1263. Huge difference in Tm of my For and Rev primers = no PCR product?!? - (reply: 11)
1264. Stable Mammalian Expression with Retrovirus - (reply: 2)
1265. Neon Transfection System - (reply: 2)
1266. help - (reply: 5)
1267. why they use isopropanal for precipitate DNA in midiprep - (reply: 5)
1268. Molecular Cloning - Cloning of PAPD PCR products (reply: 1)
1269. problems with qiagen gel extraction kit - (reply: 7)
1270. restriction enzyme flanking sequence preferance - (reply: 2)
1271. pCDH Lentivector has 500 bp insert - (reply: 5)
1272. 5' RACE for very GC-rich gene - (reply: 2)
1273. Phosphorylated primers - (reply: 5)
1274. Correct orientation of the insert - (reply: 7)
1275. screening of positive clones - colony PCR /Plasmid isolation and Restriction digestion (reply: 5)
1276. PIR 1 Competent Cells - Poor Transformation Efficiency (reply: 1)
1277. Cloning advice needed - (reply: 4)
1278. pcr amolification with long pcr enzyme mix for cloning - (reply: 1)
1279. Colony Screeing without using PCR - (reply: 8)
1280. blue white screening - (reply: 3)
1281. phosphorylate dprimers for pfx do not work - (reply: 3)
1282. help - (reply: 1)
1283. problem with yeast transformation - (reply: 2)
1284. ta cloning of pfu amplified fragment - (reply: 9)
1285. how to express the gene in opposite transcriptional direction - (reply: 3)
1286. Water for electrocompetent cells - (reply: 2)
1287. AAARGHHH, cloning driving me insane, HELP PLEASE! - UPDATE on same 5' and 3' ends (reply: 2)
1288. Bacterial growth in agar plate with ampicillin - (reply: 4)
1289. problem in ligation and cloning - (reply: 1)
1290. Electrophoresis gel boxes and geldoc system - (reply: 2)