|
931. Viral cDNA Library - (reply: 3)
932. metylated cloning vector - restriction digest of the insert (reply: 2)
933. Seeing two SDS-PAGE bands upon protein expression from pET-30b(+) - (reply: 1)
934. invitro mutagenesis using stragene kit - (reply: 4)
935. Secretion cloning vectors - (reply: 1)
936. SM10 lambda pir covers my plates - U$ 10.00 Amazon.com gift card for the most helpful feedback (reply: 4)
937. complementing a double knockout in E. coli - (reply: 1)
938. dephosphorilation of vector ends - could SAP alter sticky ends? (reply: 3)
939. Cloning two inserts at a time in a vector - Cloning two inserts at a time in a vector (reply: 2)
940. Overexpression of mouse constructs in human cell lines - some questions (reply: 6)
941. which G418 concentration and treatment period should be used? - (reply: 2)
942. HELP! cloning problem with pET22b using NdeI and XhoI sites - (reply: 4)
943. TOPO TA Cloning of WGA products? - Is this possible? (reply: 2)
944. Chemical competent E.coli always from single colony ? - (reply: 2)
945. midiprep plasmids not expressing - I achieve good yield but now the plasmids do not express (reply: 2)
946. Strange results of cloning - insert always reverse-oriented (reply: 2)
947. electroporation - (reply: 2)
948. transfection or infection - difference? (reply: 1)
949. outgrowth time andampicillin conc for low-copy numbe plasmid - (reply: 15)
950. No colonies after transformation! - (reply: 2)
951. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
952. Proportion of colonies containing DNA inserted at the cloning site - (reply: 1)
953. Help Miniprep kit SIGMA - (reply: 2)
954. Left polymerase above fridge for more than 1 week - still working? (reply: 3)
955. A question about pBR322 and pUC ori - (reply: 3)
956. Digestion of mini-prep by mistake - (reply: 3)
957. Cloning issue - (reply: 3)
958. Cohesive end filling after CIAP treatment - (reply: 2)
959. Looking for Pfu Taq for high GC-content template - (reply: 7)
960. questions about transformation - (reply: 4)
932. metylated cloning vector - restriction digest of the insert (reply: 2)
933. Seeing two SDS-PAGE bands upon protein expression from pET-30b(+) - (reply: 1)
934. invitro mutagenesis using stragene kit - (reply: 4)
935. Secretion cloning vectors - (reply: 1)
936. SM10 lambda pir covers my plates - U$ 10.00 Amazon.com gift card for the most helpful feedback (reply: 4)
937. complementing a double knockout in E. coli - (reply: 1)
938. dephosphorilation of vector ends - could SAP alter sticky ends? (reply: 3)
939. Cloning two inserts at a time in a vector - Cloning two inserts at a time in a vector (reply: 2)
940. Overexpression of mouse constructs in human cell lines - some questions (reply: 6)
941. which G418 concentration and treatment period should be used? - (reply: 2)
942. HELP! cloning problem with pET22b using NdeI and XhoI sites - (reply: 4)
943. TOPO TA Cloning of WGA products? - Is this possible? (reply: 2)
944. Chemical competent E.coli always from single colony ? - (reply: 2)
945. midiprep plasmids not expressing - I achieve good yield but now the plasmids do not express (reply: 2)
946. Strange results of cloning - insert always reverse-oriented (reply: 2)
947. electroporation - (reply: 2)
948. transfection or infection - difference? (reply: 1)
949. outgrowth time andampicillin conc for low-copy numbe plasmid - (reply: 15)
950. No colonies after transformation! - (reply: 2)
951. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
952. Proportion of colonies containing DNA inserted at the cloning site - (reply: 1)
953. Help Miniprep kit SIGMA - (reply: 2)
954. Left polymerase above fridge for more than 1 week - still working? (reply: 3)
955. A question about pBR322 and pUC ori - (reply: 3)
956. Digestion of mini-prep by mistake - (reply: 3)
957. Cloning issue - (reply: 3)
958. Cohesive end filling after CIAP treatment - (reply: 2)
959. Looking for Pfu Taq for high GC-content template - (reply: 7)
960. questions about transformation - (reply: 4)