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61. Migration of circular DNA vs circular/crosslinked peptide in gels - (reply: 2)
62. Question in gateway system, LR reaction - (reply: 5)
63. Problem with subcloning genescript synthesized genes - (reply: 2)
64. Flourescence visualization - (reply: 2)
65. Gel Purification: When is it safe to pause? - (reply: 2)
66. pUC and pSC101 vectors in the same cell - (reply: 3)
67. DNA amount for RE reaction - (reply: 3)
68. Whatman FTA cards - (reply: 1)
69. Trouble with TOPO-Cloning - (reply: 3)
70. Loss fluorescence when clone a fluorescent sensor into a differenet vector - (reply: 1)
71. Comparing DNA fragments using restriction map - (reply: 1)
72. Doing a double digest - (reply: 3)
73. Types of igation controls - (reply: 6)
74. Problem with double digest cloning - (reply: 3)
75. Weird results with bacterial transformation - (reply: 11)
76. Site Directed Mutagenesis - no product - (reply: 4)
77. Problems sequencing ligation product - (reply: 5)
78. Faster method to screeen for positive colonies. - (reply: 8)
79. 260/230 ratio, does it matter? - (reply: 4)
80. Double digestion problem with PUCsp vector - (reply: 1)
81. How much plasmid for transformation? - (reply: 8)
82. RE digestion and ligation troubleshooting. - (reply: 1)
83. replicating a mammalian expression vector in bacteria - (reply: 3)
84. Cloning of an unknown gene - (reply: 4)
85. cloning problems using in-fusion kit - (reply: 5)
86. Mutation in one chain of homodimer - (reply: 4)
87. Insert phosphorylation or digestion - which should be done first? - (reply: 4)
88. Alternative competent cells for QuikChange kit - (reply: 2)
89. Ligation Mistake - (reply: 4)
90. Cloning - Ligation problem - (reply: 3)
62. Question in gateway system, LR reaction - (reply: 5)
63. Problem with subcloning genescript synthesized genes - (reply: 2)
64. Flourescence visualization - (reply: 2)
65. Gel Purification: When is it safe to pause? - (reply: 2)
66. pUC and pSC101 vectors in the same cell - (reply: 3)
67. DNA amount for RE reaction - (reply: 3)
68. Whatman FTA cards - (reply: 1)
69. Trouble with TOPO-Cloning - (reply: 3)
70. Loss fluorescence when clone a fluorescent sensor into a differenet vector - (reply: 1)
71. Comparing DNA fragments using restriction map - (reply: 1)
72. Doing a double digest - (reply: 3)
73. Types of igation controls - (reply: 6)
74. Problem with double digest cloning - (reply: 3)
75. Weird results with bacterial transformation - (reply: 11)
76. Site Directed Mutagenesis - no product - (reply: 4)
77. Problems sequencing ligation product - (reply: 5)
78. Faster method to screeen for positive colonies. - (reply: 8)
79. 260/230 ratio, does it matter? - (reply: 4)
80. Double digestion problem with PUCsp vector - (reply: 1)
81. How much plasmid for transformation? - (reply: 8)
82. RE digestion and ligation troubleshooting. - (reply: 1)
83. replicating a mammalian expression vector in bacteria - (reply: 3)
84. Cloning of an unknown gene - (reply: 4)
85. cloning problems using in-fusion kit - (reply: 5)
86. Mutation in one chain of homodimer - (reply: 4)
87. Insert phosphorylation or digestion - which should be done first? - (reply: 4)
88. Alternative competent cells for QuikChange kit - (reply: 2)
89. Ligation Mistake - (reply: 4)
90. Cloning - Ligation problem - (reply: 3)