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Molecular-Cloning
91.
How to clone a 2A peptide between two ORFs? -
(reply: 2)
92.
preparing a vector for transfection -
(reply: 2)
93.
Ligation of two PCR products -
(reply: 1)
94.
TA Cloning -
(reply: 11)
95.
Troubleshootting lentiviral transduction. -
(reply: 3)
96.
Trouble with PCR using ligation mix as template? -
(reply: 3)
97.
Suitable ratio of vector and insert in cloning -
(reply: 5)
98.
The right lentivirus plasmid for overexpression in human cells -
(reply: 7)
99.
When designing a Mammalian Expression Vector, Should you include the complete CD -
(reply: 3)
100.
plasmid repositories -
(reply: 2)
101.
How to insert PreScission protease into a vector -
(reply: 1)
102.
Why the insert become shorter after inserting into plasmid? -
(reply: 2)
103.
Bacteria grow on antibiotic selective plate but plasmid extracted showed no dna -
(reply: 6)
104.
Thermo scientific T4 DNA ligase product help -
(reply: 3)
105.
Cloning a big insert in a small vector -
(reply: 2)
106.
Transformation E.Coli help -
(reply: 2)
107.
Exonucleases that won't harm circular dsDNA -
(reply: 1)
108.
How to produce transgenic mice? -
(reply: 10)
109.
No colonies on plate after liagtion -
(reply: 7)
110.
Question: Can I cut out rb glob PA Terminator -
(reply: 1)
111.
Low Yield Miniprep -
(reply: 5)
112.
question about Clonetech Retro-X Tet-on system -
(reply: 3)
113.
Clonetech Retro-X Tet-on system -
(reply: 1)
114.
quiaquick gel extraction troubleshooting -
(reply: 1)
115.
Insert direction in TA cloning -
(reply: 2)
116.
Transformation in Staphylococcus epidermidis -
(reply: 1)
117.
Plasmids recombining -
(reply: 3)
118.
troubleshooting for a simple SDM -
(reply: 2)
119.
Should I proceed with clone selection? -
(reply: 2)
120.
Need help in codon optimization... -
(reply: 1)
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