|
1051. A suitable vector for the cloning of multiple genes for protein expression - (reply: 4)
1052. looking for binding site - software? (reply: 1)
1053. Insertion of loxp/avoiding SINE - (reply: 1)
1054. Question: Ligation Problem.... - (reply: 2)
1055. Plasmid-Stability? Fragment-Deletion? I'm in dire need to identify and solve - (reply: 20)
1056. Topo directional cloning - pENTR/D-TOPO (reply: 1)
1057. trouble in TOPO TA sequencing - (reply: 1)
1058. Large DNA extraction from an agarose gel - (reply: 8)
1059. Dna cloning using NdeI/BamHI using pET11 vector - (reply: 4)
1060. primer Tm is too high, how tu get pcr product - help! (reply: 21)
1061. Ligation problem, religation of backbone without any insert? - (reply: 3)
1062. problem with pLK01 plasmid ligation - (reply: 2)
1063. Mini prep streaking - basic question (reply: 3)
1064. In-fusion joining few parts together - possible? ideas? (reply: 3)
1065. TOPO cloning problem - (reply: 4)
1066. How to delete a domain in a gene? - cloned plasmid (reply: 12)
1067. Red/ET recombination - (reply: 2)
1068. multiple origins of replication - (reply: 2)
1069. Dye in PCR Buffer - Inhibiting downstream techniques? - ligation/digestion/cloning (reply: 7)
1070. Single Restriction Site cloning - Cloning (reply: 6)
1071. LR Reaction - Unwanted Homologous Recombinations - (reply: 1)
1072. 3-(N-morpholino)propanesulfonic acid: MOPS - (reply: 6)
1073. EcoRI cloning giving only inverted inserts!!?? - (reply: 3)
1074. BamHI and SacI double digest - 100% activity in NEBuffer 4 but why not recommended? (reply: 5)
1075. Cleaning RNase - How do i clean out the RNase from my genomic DNA (reply: 1)
1076. cloning very long fragments into vectors using T/A cloning method, - (reply: 2)
1077. Agarose gel electrophoresis question - (reply: 1)
1078. amount of plasmid for transformation and fusion protein expression - (reply: 1)
1079. My white colonies turned blue in frig! Why? - (reply: 6)
1080. Digestion problem XbaI, methylation sensitive site - (reply: 3)
1052. looking for binding site - software? (reply: 1)
1053. Insertion of loxp/avoiding SINE - (reply: 1)
1054. Question: Ligation Problem.... - (reply: 2)
1055. Plasmid-Stability? Fragment-Deletion? I'm in dire need to identify and solve - (reply: 20)
1056. Topo directional cloning - pENTR/D-TOPO (reply: 1)
1057. trouble in TOPO TA sequencing - (reply: 1)
1058. Large DNA extraction from an agarose gel - (reply: 8)
1059. Dna cloning using NdeI/BamHI using pET11 vector - (reply: 4)
1060. primer Tm is too high, how tu get pcr product - help! (reply: 21)
1061. Ligation problem, religation of backbone without any insert? - (reply: 3)
1062. problem with pLK01 plasmid ligation - (reply: 2)
1063. Mini prep streaking - basic question (reply: 3)
1064. In-fusion joining few parts together - possible? ideas? (reply: 3)
1065. TOPO cloning problem - (reply: 4)
1066. How to delete a domain in a gene? - cloned plasmid (reply: 12)
1067. Red/ET recombination - (reply: 2)
1068. multiple origins of replication - (reply: 2)
1069. Dye in PCR Buffer - Inhibiting downstream techniques? - ligation/digestion/cloning (reply: 7)
1070. Single Restriction Site cloning - Cloning (reply: 6)
1071. LR Reaction - Unwanted Homologous Recombinations - (reply: 1)
1072. 3-(N-morpholino)propanesulfonic acid: MOPS - (reply: 6)
1073. EcoRI cloning giving only inverted inserts!!?? - (reply: 3)
1074. BamHI and SacI double digest - 100% activity in NEBuffer 4 but why not recommended? (reply: 5)
1075. Cleaning RNase - How do i clean out the RNase from my genomic DNA (reply: 1)
1076. cloning very long fragments into vectors using T/A cloning method, - (reply: 2)
1077. Agarose gel electrophoresis question - (reply: 1)
1078. amount of plasmid for transformation and fusion protein expression - (reply: 1)
1079. My white colonies turned blue in frig! Why? - (reply: 6)
1080. Digestion problem XbaI, methylation sensitive site - (reply: 3)