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1651. CIP required for double digestion of plasmid? - (reply: 3)
1652. Help with cloning a plasmid that contains repeats! - (reply: 2)
1653. To what extent competent cells be thawed before transformation? - (reply: 4)
1654. Problem with the transformation - I could find the insert in plasmid when I did Gel electrophoresis (reply: 1)
1655. size of gene insert wrong? - (reply: 3)
1656. Viral Shuttle Plasmid Restriction digests - (reply: 1)
1657. yeast transformation - how to make a thermotolerant yeast (reply: 4)
1658. need help with klenow - (reply: 4)
1659. Subcloning into pBluescript - (reply: 6)
1660. How to control cloned cDNA expression level in cells? - (reply: 8)
1661. PCR-based Site-directed mutagenesis - clear bend on the gel but no colony on the plate (reply: 7)
1662. Equation for calculating insert:vector ratio in ligation reaction - (reply: 5)
1663. Help with 3-way ligation - and calculating molar ratios for 3-way ligations... (reply: 11)
1664. Gel purification of vector DNA - low yield - (reply: 26)
1665. ligation and transformation - need help with trouble shooting!!!! (reply: 7)
1666. Identifying cDNA insert - Basic help needed (reply: 2)
1667. gel purification or ligation problem - (reply: 3)
1668. Sequential restriction digestion of plasmid - (reply: 58)
1669. Site-directed mutagenesis - nucleotides insertion (reply: 1)
1670. Troublshooting failed transformation - pLEASE hELP (reply: 19)
1671. Transformation question - (reply: 9)
1672. Cloning trouble - (reply: 5)
1673. Problem cloning oligo into double digested vector - (reply: 9)
1674. Has anyone had experience with GM2163 (dam-) cells? - the enzyme BclI is blocked by dam (reply: 1)
1675. endonuclease contamination in mini-preps - (reply: 8)
1676. S-O-S Ligation Not Working - No Colonies After Ligation (reply: 5)
1677. cloning 1052bp fragment - no colonies (reply: 11)
1678. What is the biggest insert for a viral vector? - (reply: 15)
1679. Stored bacteria lost plasmids? - (reply: 12)
1680. After Double Digestion, there is one band on the very top. What is it? - (reply: 2)
1652. Help with cloning a plasmid that contains repeats! - (reply: 2)
1653. To what extent competent cells be thawed before transformation? - (reply: 4)
1654. Problem with the transformation - I could find the insert in plasmid when I did Gel electrophoresis (reply: 1)
1655. size of gene insert wrong? - (reply: 3)
1656. Viral Shuttle Plasmid Restriction digests - (reply: 1)
1657. yeast transformation - how to make a thermotolerant yeast (reply: 4)
1658. need help with klenow - (reply: 4)
1659. Subcloning into pBluescript - (reply: 6)
1660. How to control cloned cDNA expression level in cells? - (reply: 8)
1661. PCR-based Site-directed mutagenesis - clear bend on the gel but no colony on the plate (reply: 7)
1662. Equation for calculating insert:vector ratio in ligation reaction - (reply: 5)
1663. Help with 3-way ligation - and calculating molar ratios for 3-way ligations... (reply: 11)
1664. Gel purification of vector DNA - low yield - (reply: 26)
1665. ligation and transformation - need help with trouble shooting!!!! (reply: 7)
1666. Identifying cDNA insert - Basic help needed (reply: 2)
1667. gel purification or ligation problem - (reply: 3)
1668. Sequential restriction digestion of plasmid - (reply: 58)
1669. Site-directed mutagenesis - nucleotides insertion (reply: 1)
1670. Troublshooting failed transformation - pLEASE hELP (reply: 19)
1671. Transformation question - (reply: 9)
1672. Cloning trouble - (reply: 5)
1673. Problem cloning oligo into double digested vector - (reply: 9)
1674. Has anyone had experience with GM2163 (dam-) cells? - the enzyme BclI is blocked by dam (reply: 1)
1675. endonuclease contamination in mini-preps - (reply: 8)
1676. S-O-S Ligation Not Working - No Colonies After Ligation (reply: 5)
1677. cloning 1052bp fragment - no colonies (reply: 11)
1678. What is the biggest insert for a viral vector? - (reply: 15)
1679. Stored bacteria lost plasmids? - (reply: 12)
1680. After Double Digestion, there is one band on the very top. What is it? - (reply: 2)