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1111. Vectors with special MCS - (reply: 5)
1112. Cloning <100 bp fragments into a vector - (reply: 7)
1113. Help! my plasmid size changed after ligation - (reply: 5)
1114. What is the BGH gene for in a expression vector? - What is the BGH gene for in a expression vector? Thanks a lot! (reply: 4)
1115. Restriction digestion of gDNA for phage library - (reply: 5)
1116. how i can get insert back? - (reply: 1)
1117. DNA precipitation- Why EtOH? - Why we use EtOH and NaOAc together? (reply: 1)
1118. New to cloning -- strangely "mature" colonies after ON growth. Any idea - (reply: 1)
1119. Chromosome Specific Enzymes - Mammalian enzymes that cut only one chromosome in genome (reply: 1)
1120. mammalian expression vector - (reply: 2)
1121. Electroporation doesn't like my plasmid??? - (reply: 4)
1122. low yeild fo RNA after invitro transcription - (reply: 4)
1123. No expresion of transgene - Retroviral vector (reply: 1)
1124. plasmids - plasmids for stable cell line construction (reply: 2)
1125. empty vectors more than the insert - (reply: 6)
1126. Restriction enzyme digestion problem - (reply: 2)
1127. low copy plasmid - how to improve the yield? (reply: 1)
1128. How to anneal more than 2 oligos???? - Protocol wanted that allows the annealing of several oligos (reply: 2)
1129. Need help with 7-way ligation. - Help make 7-way reliable cloning strategy! (reply: 1)
1130. Ligation/Transformation Troubles - Lentiviral vector (reply: 9)
1131. Is it possible to ligate blunt and sticky ends at the same time? - newbie mistake... Please help!! (reply: 11)
1132. Cloning Help!! - It is getting very annoying... (reply: 13)
1133. restriction digestion - (reply: 7)
1134. sequence errors created during cloning - (reply: 6)
1135. mutagenesis - mutate a vector by deleting its promoter site (reply: 1)
1136. the pyrithiamine - (reply: 5)
1137. Please advise on my transgene strategy :) - Thanks! (reply: 4)
1138. Retroviral Vector Construction - Simultaneous gene expression and shRNA knockdown? (reply: 8)
1139. What is the role of DMSO in yeast transformation - (reply: 1)
1140. ligation problem - (reply: 3)
1112. Cloning <100 bp fragments into a vector - (reply: 7)
1113. Help! my plasmid size changed after ligation - (reply: 5)
1114. What is the BGH gene for in a expression vector? - What is the BGH gene for in a expression vector? Thanks a lot! (reply: 4)
1115. Restriction digestion of gDNA for phage library - (reply: 5)
1116. how i can get insert back? - (reply: 1)
1117. DNA precipitation- Why EtOH? - Why we use EtOH and NaOAc together? (reply: 1)
1118. New to cloning -- strangely "mature" colonies after ON growth. Any idea - (reply: 1)
1119. Chromosome Specific Enzymes - Mammalian enzymes that cut only one chromosome in genome (reply: 1)
1120. mammalian expression vector - (reply: 2)
1121. Electroporation doesn't like my plasmid??? - (reply: 4)
1122. low yeild fo RNA after invitro transcription - (reply: 4)
1123. No expresion of transgene - Retroviral vector (reply: 1)
1124. plasmids - plasmids for stable cell line construction (reply: 2)
1125. empty vectors more than the insert - (reply: 6)
1126. Restriction enzyme digestion problem - (reply: 2)
1127. low copy plasmid - how to improve the yield? (reply: 1)
1128. How to anneal more than 2 oligos???? - Protocol wanted that allows the annealing of several oligos (reply: 2)
1129. Need help with 7-way ligation. - Help make 7-way reliable cloning strategy! (reply: 1)
1130. Ligation/Transformation Troubles - Lentiviral vector (reply: 9)
1131. Is it possible to ligate blunt and sticky ends at the same time? - newbie mistake... Please help!! (reply: 11)
1132. Cloning Help!! - It is getting very annoying... (reply: 13)
1133. restriction digestion - (reply: 7)
1134. sequence errors created during cloning - (reply: 6)
1135. mutagenesis - mutate a vector by deleting its promoter site (reply: 1)
1136. the pyrithiamine - (reply: 5)
1137. Please advise on my transgene strategy :) - Thanks! (reply: 4)
1138. Retroviral Vector Construction - Simultaneous gene expression and shRNA knockdown? (reply: 8)
1139. What is the role of DMSO in yeast transformation - (reply: 1)
1140. ligation problem - (reply: 3)