541. Problems in RNAi construction by using pUC19 - (reply: 3)
542. Unable to clone in E. coli cells - (reply: 3)
543. Why tranform using DH5 alpha -> SM10 λpir?? - (reply: 3)
544. plasmid rearrangement or sabotage - (reply: 2)
545. pLDR20 Vector - (reply: 1)
546. surprising behavior of transformants needs immediate advice! - (reply: 8)
547. cloning antisense promoter in pGL3Basic - (reply: 2)
548. PhiX174 DNA - (reply: 1)
549. Competent bacterial cells - (reply: 2)
550. Replacing puramycin in a pLKO1.puro - (reply: 1)
551. colony cracking for screening of positive clones - (reply: 2)
552. deletion of large fregments - (reply: 3)
553. Linearising plasmid before transfection? - (reply: 2)
554. Smear in plasmid sample - (reply: 9)
555. about a book - (reply: 1)
556. Cloning Problem! I need help - (reply: 3)
557. Help designing primers for use in In-fushion cloning - (reply: 4)
558. Repeat of desired mutation in cloning product - (reply: 1)
559. vector dephosphorylation - (reply: 5)
560. ura3 mutant yeast strain grows on SD-uracil- - (reply: 1)
561. DH10BAC - (reply: 3)
562. Accuracy of Nanodrop to measure plasmid concentration - (reply: 6)
563. protein is not expessing in HEK 293 cells - (reply: 2)
564. Restriction problems - (reply: 4)
565. cloning a gene in to a plasmid - (reply: 5)
566. no product on gel after restriction digestion - (reply: 3)
567. Gateway technology. - (reply: 4)
568. Primer concentration for cDNA synthesis using GSP - (reply: 1)
569. cloning from cDNA but got a much shorter PCR product - (reply: 4)
570. GeneArt vs Quikchange - (reply: 1)

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