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1351. PCR and then ligation - (reply: 7)
1352. cloning problems in C. elegans expression plasmid - Trying to use Fire vector pPD49.83 (reply: 1)
1353. 3 way ligation help needed - (reply: 10)
1354. Cloning and Expression Method Survey - Get some ideas from you all (reply: 1)
1355. Cloning woes - Running out of ideas! - (reply: 7)
1356. What fields of research other than microbiology work with bacteria and - (reply: 1)
1357. How to inoculate bacteria? - (reply: 10)
1358. What retrovirus vetor can have 7.5kb insert - (reply: 1)
1359. need help in my Fse1 restriction ezyme - (reply: 4)
1360. Insert problems with TA cloning - (reply: 1)
1361. Surface expression by tansfection of CHO - (reply: 3)
1362. please help!!! with transformation - (reply: 10)
1363. sequencing primer: T7 or T7promoter? - (reply: 4)
1364. Unstable clone? - (reply: 1)
1365. Restriction enzymes - (reply: 3)
1366. Problems facing in cloning with pBI121 vector - (reply: 1)
1367. Failed Transformation of Tuner (DE3) cells - (reply: 1)
1368. Is my insert present??? - (reply: 17)
1369. Need help for restriction enzymes - (reply: 5)
1370. Heat inactivation/Gel purification or both? - Normally I purify from gel. Should I inactivate the enzymes? (reply: 2)
1371. Mini prep - (reply: 4)
1372. adding koazak sequence to primer - (reply: 1)
1373. DNA ligation - (reply: 2)
1374. adding C terminal tag to reverse primer - (reply: 1)
1375. glycerol stocks - (reply: 10)
1376. doubts in sequence submission to NCBI - (reply: 2)
1377. Concentration of DNA for Transformation - (reply: 1)
1378. Restriction digest - (reply: 7)
1379. Help! Double restriction digestion failed.... - (reply: 6)
1380. Kozak sequences.. - (reply: 1)
1352. cloning problems in C. elegans expression plasmid - Trying to use Fire vector pPD49.83 (reply: 1)
1353. 3 way ligation help needed - (reply: 10)
1354. Cloning and Expression Method Survey - Get some ideas from you all (reply: 1)
1355. Cloning woes - Running out of ideas! - (reply: 7)
1356. What fields of research other than microbiology work with bacteria and - (reply: 1)
1357. How to inoculate bacteria? - (reply: 10)
1358. What retrovirus vetor can have 7.5kb insert - (reply: 1)
1359. need help in my Fse1 restriction ezyme - (reply: 4)
1360. Insert problems with TA cloning - (reply: 1)
1361. Surface expression by tansfection of CHO - (reply: 3)
1362. please help!!! with transformation - (reply: 10)
1363. sequencing primer: T7 or T7promoter? - (reply: 4)
1364. Unstable clone? - (reply: 1)
1365. Restriction enzymes - (reply: 3)
1366. Problems facing in cloning with pBI121 vector - (reply: 1)
1367. Failed Transformation of Tuner (DE3) cells - (reply: 1)
1368. Is my insert present??? - (reply: 17)
1369. Need help for restriction enzymes - (reply: 5)
1370. Heat inactivation/Gel purification or both? - Normally I purify from gel. Should I inactivate the enzymes? (reply: 2)
1371. Mini prep - (reply: 4)
1372. adding koazak sequence to primer - (reply: 1)
1373. DNA ligation - (reply: 2)
1374. adding C terminal tag to reverse primer - (reply: 1)
1375. glycerol stocks - (reply: 10)
1376. doubts in sequence submission to NCBI - (reply: 2)
1377. Concentration of DNA for Transformation - (reply: 1)
1378. Restriction digest - (reply: 7)
1379. Help! Double restriction digestion failed.... - (reply: 6)
1380. Kozak sequences.. - (reply: 1)