Please anybody can explain the cloning related to plant vector construction - Plant vector construction (Apr/16/2009 )
In Cloning what is the use of polylinker usually they wil use pUC plasmids what is this ?
Can anybody explain
A polylink is a DNA sequence that contains many of the common restriction sites. Inserting it into a vector allows you to clone virtually any restriction cut piece of DNA into that vector.
thanking u very much
can u give one example I am new to this area
Examine the sequene of the cloning site of a common cloning vector such as pUC19. When these were first developed (1980s I think) there were papers published on them, which Pubmed could probably find, for example:
The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
Vieira J, Messing J
Gene. 1982 Oct;19(3):259-68.
PMID: 6295879
phage434 on Apr 17 2009, 09:36 AM said:
The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
Vieira J, Messing J
Gene. 1982 Oct;19(3):259-68.
PMID: 6295879
thanking u very much sir
what are all the basic things need to know before starting the cloning work?