how to store LB-agar-antibiotic- X-gal-IPTG plate - (Feb/13/2012 )
Hi, I want to make some how to store LB-agar-chloramphenicol-X-gal-IPTG plate, but I don't know how should I store them and how long it can last.
Would you please give me some suggestions?
Thanks a lot!
-joy123-
Store at 4 deg C sealed (in a bag is fine) to prevent water loss. The X-gal will degrade relatively fast, I think about 2-3 weeks will be the maximum.
-bob1-
bob1 on Mon Feb 13 22:03:07 2012 said:
Store at 4 deg C sealed (in a bag is fine) to prevent water loss. The X-gal will degrade relatively fast, I think about 2-3 weeks will be the maximum.
Thanks very much for your advice!
-joy123-
You may find S-Gal (from Sigma) much easier to use for differentiating bla+ strains. It's a straight substitute for X-Gal, and I think it works a lot better.
-phage434-