Gibson/SLIC question - (Mar/05/2014 )

I'm a bit confused regarding Gibson assembly or SLIC. Using an exonuclease should result in regression of both 5' ends, right? Why is in all schemes only one of them degraded ( https://www.neb.com/products/e5510-gibson-assembly-cloning-kit )? Isn't there a chance that the whole PCR product will be eventually degraded? Or are they using modified primers?

Thanks,

rsm

-Rsm-

Sure, that makes sense. What I mean is that both ends of the vector and the PCR product should be chewed away, such as

5'--------------------------------3' goes to 5'-----------------3' and not 5'-------------------------3' as they show in the picture.

3'--------------------------------5' 3'------------------5' 3'-------------5'

Why is the left arm of the DNA "protected" in the pic? ie why is only one DNA strand degraded?

-Rsm-