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Mini prep of frozen bacteria cells - (Jun/26/2011 )

I did not spin down bacteria pellets before keeping into -21 C freezer for 2 days. Can I still do mini prep?

Thank you.

-Wisoot-

Wisoot on Mon Jun 27 02:14:44 2011 said:


I did not spin down bacteria pellets before keeping into -21 C freezer for 2 days. Can I still do mini prep?

Thank you.

Hola, yes plasmid are inside, and only you have a bit of bacteria broken, defrost the pellet and dissolve in the resuspension buffer, after with the lisys buffer you have a part of the work made. Buena suerte

-protolder-

Thanks a lot Veteran.

-Wisoot-