Mini prep of frozen bacteria cells - (Jun/26/2011 )
I did not spin down bacteria pellets before keeping into -21 C freezer for 2 days. Can I still do mini prep?
Thank you.
-Wisoot-
Wisoot on Mon Jun 27 02:14:44 2011 said:
I did not spin down bacteria pellets before keeping into -21 C freezer for 2 days. Can I still do mini prep?
Thank you.
Hola, yes plasmid are inside, and only you have a bit of bacteria broken, defrost the pellet and dissolve in the resuspension buffer, after with the lisys buffer you have a part of the work made. Buena suerte
-protolder-
Thanks a lot Veteran.
-Wisoot-