Recombination protocol needed - (Jan/25/2012 )

Hi all,

I want to clone 2,2kb fragments into 15 kb vector by bacteria recombination, because I have tried several time by ''classic'' clonage (restriction enzyme and ligation) and it doesn't work.

Thanks,

http://web.ncifcrf.gov/research/brb/protocol/Protocol1_DY380.pdf

this should be a good starter!

Regards,
p

Thank you so much for your help, pDNA.

dan