Protocol Online logo
Top : New Forum Archives (2009-): : Molecular Cloning

Can you engineer a kinetochore in a BAC so that it segregates evenly - (Sep/01/2013 )

I'm trying to make a BAC that can separate just like any other chromosome in eukaryotic mitosis/meoisis. 

-Ahrenhase-

Hmmm, you do ask the hard questions.  You could try a YAC rather than a BAC?  But I have no idea really.

-bob1-

bob1 on Tue Sep 3 10:30:53 2013 said:

Hmmm, you do ask the hard questions.  You could try a YAC rather than a BAC?  But I have no idea really.

And you do give the good answers.  Thanks, YACs would make more sense.

-Ahrenhase-

Phew, lucky guess.

-bob1-