Cleaning loading dye for PCR sequencing - (Sep/18/2015 )
Hi, I sometimes use a polymerase with added loading green dye, has anyone been successful in cleaning it using ethanol precipitation or phenol:chloroform elution?
I tried ethanol precipitation and the resulting elutant was still pretty green.
Thanks
-Raygoza-
I think it is impossible to clean the green dye by that way, you'd better use new polymerase.
-Andrea Fortina-