Selecting bacteria with Puromycin - (Dec/23/2015 )

I want to use a plasmid with puromycin to select both bacteria and mammalian cells.

I learnt puromycin is not very effective for selecting bacteria. 

However, they say, under particular applications it can be used for selecting bacteria.

They mentioned the concentration as 125 ug/ml.

They say precise pH adjustment is required.

But they did not say the value of the pH.

Has anybody used puromycin for selecting bacteria? 

At what pH is the the drug effective against bacteria (e coli)?

Your response(s) will be very much appreciated

Sincerely,

Kwesi

I never use puromycin for bacteria, if you want to select bacteria obtain some plasmid, maybe you could check whether the plasmid has other selection marker. Generally, shuttle plasmid has two selectable marker at least, one for bacteria and one for cell.