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Primer Related Discussions
  1. ITP substitutes... what? - can I add ITP to my degenerated primers to lower the degeneracy? (reply: 2)
  2. need help...how to compare mouse vs human sequences for primer design - (reply: 2)
  3. PCR problem (great different of Tm in primers) - (reply: 3)
  4. Create an artificial restriction site for a endonuclease and 53bp long primer? - (reply: 2)
  5. Tm of PCR primers: one 68, the other 58. Impossible to succeed? - (reply: 3)
  6. Primer design for PCR following ChIP - (reply: 3)
  7. How to name primers? - Any official nomenclature systems to name primers? (reply: 4)
  8. Primer dimers!? HELP - (reply: 8)
  9. MSP primer does not work - (reply: 5)
  10. How primers can be measured? - (reply: 6)
  11. RE sites on the primer. - How can I check for restriction sites on a primer sequence? (reply: 2)
  12. What are normalisation primers for the rt-PCR? - Im going to order the mirVana rt-PCR kit for miRNAs (reply: 1)
  13. RNA contamination in primers - (reply: 2)
  14. how 2 design rev.transPCR primer? - need functioning protocol.... (reply: 1)
  15. methylnick.please help me with MSP primer designing! - (reply: 3)
  16. concentration of primers - (reply: 1)
  17. About DAPK1 MSP primer design. - About MSP primer design. (reply: 4)
  18. Primer design from mRNA or DNA - Confused about what sequence to use for design of GSP and pPCR primers (reply: 2)
  19. deisgning primers with restriction site - (reply: 7)
  20. Questions about primer design for BSP in plant - shouldn't we take the mC in CHG CHH context into consideration? (reply: 3)
  21. cDNA primer help - (reply: 1)
  22. cDNA and primer Design Help - (reply: 6)
  23. Bisulfite sequencing reproducibility - overlapping PCR amp primers give varying results (reply: 2)
  24. Primer design program for Methylight - (reply: 3)
  25. primer design software - primes for full lenth gene (reply: 3)
  26. Please help me design MSP primer! - (reply: 4)
  27. What grade of primers for real-time TaqMan PCR assays? - (reply: 1)
  28. Tm of the primers - company sends primers with lower Tm than expected (reply: 3)
  29. RT-PCR primer design - RT-PCR primer design...need ur help (reply: 3)
  30. RT-PCR primer design - RT-PCR primer design...need ur help (reply: 1)
  31. primer sequence - reverse primer sequence (reply: 3)
  32. cDNA and additional bands when run on gel - I get a band o fthe same Mw regardless of the primers I use (reply: 2)
  33. How to synthesize your own oligo-dT primer - (reply: 4)
  34. Designing Primers - (reply: 1)
  35. mrna detection with sequence specific primers - (reply: 2)
  36. Standard sequencing primers - how to find which sequencing primers bind to my DNA? (reply: 2)
  37. internal control primers for plants - (reply: 14)
  38. qRT-PCR primer design - (reply: 1)
  39. Where do you get your PCR primers from? - (reply: 3)
  40. PCR primers + large mRNA amplification - (reply: 5)
  41. PCR Primers for introducing tag - (reply: 5)
  42. help design primer - (reply: 6)
  43. BLAST Help , Designing primers... - (reply: 5)
  44. Different efficiency in different samples but the same primer - (reply: 1)
  45. Primer design and degeneracy - Are synthesized sets identical? (reply: 2)
  46. primers amplify additional band (!) - (reply: 1)
  47. resuspended primers in water - is this a problem?! (reply: 5)
  48. Designing the primers for qRT PCR - Query - (reply: 14)
  49. primers sequence - (reply: 2)
  50. primer designing - (reply: 1)
  51. How to design PCR primer to amplify a very long sequence(about 9.7kb) - I want to amplify cDNA genome of HCV. It's about 9.7kb long (reply: 2)
  52. Primer Design for nucleotide sequence - (reply: 1)
  53. Primer dimer problem in MSP - (reply: 2)
  54. Attaching DNA bases - Creating primers and plasmids? (reply: 3)
  55. Primer doubts - (reply: 2)
  56. Why the addition of restriction sites to primers? - (reply: 3)
  57. Primer desing for transcript variant - (reply: 2)
  58. Primer analysis sites - Any suggestions? (reply: 3)
  59. RAPD and viruses - RAPD using very very short primers (reply: 4)
  60. please help me to be sure from design primer have it - (reply: 3)
  61. resuspend Primers in TE or in water? - (reply: 7)
  62. manual primer desgining - (reply: 2)
  63. BLAST search of primer sequence - (reply: 3)
  64. newbie question on Primer design for MSP - (reply: 2)
  65. Oligo dT primer - oligo dT primer with non-T priming 3´ or with 5´ (reply: 3)
  66. Confirming published methylation primers - (reply: 1)
  67. Help designing primers; also help with viewing GenBank - (reply: 3)
  68. Primer design - How to - am a novice ? (reply: 6)
  69. Primer optimization (with probes) - Urges... (reply: 2)
  70. SNP - How to design the SNP primer (reply: 1)
  71. Choosing Primer: random or Olig-dt ? - (reply: 2)
  72. how to synthesize the pcr primers - (reply: 3)
  73. designing degenerate primer - (reply: 2)
  74. incorporation of restriction site in Primers - Help to design primer to clone gene in frame with fluorescent tag (reply: 2)
  75. how to design pcr primers - (reply: 7)
  76. RT-PCR primer design and in silico PCR - (reply: 4)
  77. Primer extension and sequencing from total RNA - (reply: 1)
  78. primer design - over expression (reply: 1)
  79. PCR bias with BSP primers - (reply: 1)
  80. Primer design and tolerances - (reply: 3)
  81. primer design - (reply: 2)
  82. help with degenerate primer design - (reply: 1)
  83. PCR: Big Primers = Big Problems (for me) - I'm really not sure what's going on in those poor little tubes... (reply: 2)
  84. mutations in primer sequence - (reply: 2)
  85. Nested primers - (reply: 5)
  86. Primer design - Primer design for PCR with TE sites (reply: 2)
  87. Reverse transcriptation for qPCR with some specific primers. Should I use primer - (reply: 2)
  88. primer conditions for expression work - (reply: 5)
  89. Question about primer design targeting GC-rich region - (reply: 2)
  90. promoter pcr using primers with restriction sites giving trouble - (reply: 7)
  91. how to optimize primer and cDNA concentration for real-time - (reply: 2)
  92. primer Question pVax1 RE / Kozak - (reply: 1)
  93. Help -designing primers for real time RT-PCR - (reply: 1)
  94. Primer design for SNP detection - (reply: 2)
  95. Bad primer efficiency during multiplex qPCR - (reply: 1)
  96. max primer length - 85 nt too long? - (reply: 5)
  97. different transcription from 5' UTR than 3' UTR primers - (reply: 8)
  98. recommended primers for caspase 8 & 9 - human (reply: 4)
  99. UBC primer PCR - (reply: 1)
  100. Optimising bisulphite PCR primers - (reply: 4)
  101. Primer design help - secondary structures - Please help me troubleshoot? (reply: 12)
  102. Determine specific primers and transgene copy number by PCR - (reply: 1)
  103. optimization of PCR conditions - to know that primers are specific and detect copy number (reply: 1)
  104. Any Universal Primer for 3' of cDNAs of all mRNAs? - (reply: 2)
  105. better primer for cDNA synthesis in real time pcr - (reply: 1)
  106. can you judge a 22bp primer based on the 3' 10bp? - (reply: 6)
  107. Getting primer dimers in RT-PCR on and off? - (reply: 2)
  108. conc of cDNA to be used - PCR to test primers (reply: 5)
  109. Primer for all HPV - (reply: 4)
  110. please help me check BSP primers. - (reply: 6)
  111. Lengthy primers - (reply: 11)
  112. primer design - how far apart Tms could be? (reply: 4)
  113. design primers - (reply: 3)
  114. a problem about the Tm of the BSP primer - (reply: 2)
  115. Primers distinguishing splicing alternatives - (reply: 1)
  116. primer design from mRNA - (reply: 12)
  117. who can give me a hand to check the BSP primer? - (reply: 5)
  118. Please help me design BSP primer. - (reply: 14)
  119. Primer design: how do I add a restriction site to my primer? - (reply: 2)
  120. Storing primers...together? - (reply: 3)
  121. Genomic DNA fragment labeling by random priming - (reply: 1)
  122. different length for primers amplifying - (reply: 2)
  123. primer dilution - primer dilution from picomoles to micromolar (reply: 2)
  124. Primer concentration is quantitative Real-time PCR - (reply: 1)
  125. primer dimer annealing temperature - (reply: 2)
  126. design primers with custom end - To clone my gene into the MCS as fusions to the GFP tag (reply: 5)
  127. TetR primer - (reply: 1)
  128. some primer dilution calculations - (reply: 7)
  129. primer tm with mismatch? - (reply: 6)
  130. TOPO primer choice for sequencing - (reply: 3)
  131. Can do reverse transcription by gene-specific primers? - (reply: 3)
  132. Anchored oligo dT and Thermostable reverse transcriptase - temperature compatibility between RT and oligo dT priming (reply: 8)
  133. primer design with RE sites - (reply: 4)
  134. primer desighning with RE sites - (reply: 5)
  135. Primer design (long transcripts) - (reply: 2)
  136. Primers for Real time PCR - Really need some advice (reply: 1)
  137. Stem-loop RT primers: how to anneal? - (reply: 1)
  138. second strand cDNA synth without primer? - (reply: 2)
  139. do you put two pairs primers in one tube when you do site mutations? - (reply: 2)
  140. General question about restriction sequences in PCR primers - (reply: 3)
  141. GAPDH primer sequence - cloning of huGAPDH gene (reply: 2)
  142. chip assay primers - chip assay primers (reply: 2)
  143. how to add bases to the primer restriction sites - (reply: 2)
  144. Questions about Stratagene QickChange Multi-mutagenesis - about how many primers and template DNA size (reply: 5)
  145. primer design - (reply: 3)
  146. How to ressuspend primers for Sybr? - (reply: 3)
  147. simple primer calculation - (reply: 5)
  148. picking primers - (reply: 4)
  149. 16s primers and PCR - (reply: 1)
  150. primer/probe mix for standard generation? - (reply: 2)
  151. Primers from RT-PCR database - RT-PCRdatabase primers giving primer dimers (reply: 1)
  152. restriction enzyme site with primers - (reply: 4)
  153. Help. Trying to sequence my plasmids, but the primers refuse to prime! - (reply: 1)
  154. weird problem with Primers! - problem with the gene sequence i used to design my primers (reply: 4)
  155. Forward n reversre primer Tm difference is 10 degree - (reply: 1)
  156. Primer mismatch bother me! - when enzyme sites added at 5' ends, mismatch increases. (reply: 1)
  157. primer design (downloadable) programs - not online tools (reply: 4)
  158. figure out the size of amplicon using known primer - (reply: 3)
  159. Primer concentration... - (reply: 5)
  160. microgram per microlitre primer to molarity conversion.. - calculation plss (reply: 1)
  161. Long PCR primers for epitope tagging - where to order? (reply: 3)
  162. ***MSP Primer Troubleshooting Specificity*** - (reply: 6)
  163. No PCR product with verified primers on templates - Where is the bug???? (reply: 2)
  164. How good are my PCR primers? can I go with it?! - (reply: 2)
  165. QPCR primer Pairs - (reply: 1)
  166. primer design for unknown cDNA sequence - (reply: 1)
  167. touchdown qPCR - using degenerative primers (reply: 2)
  168. primer Tm for PCR mutagenesis - (reply: 1)
  169. Quikchange mutagenesis primer and PCR design - (reply: 3)
  170. Primer design - Issues with GAPDH primer blast search (reply: 2)
  171. primers dont match FASTA gene sequence... 'm confused - (reply: 11)
  172. sequence retrieval for primer design - (reply: 2)
  173. Primer conc - (reply: 6)
  174. Does anyone know how to design LUX primers - (reply: 2)
  175. Cox-2 primer for PCR - method how to search good cDNA in Pubmed (reply: 1)
  176. PCR primers for CRISPR - primer3 isn't helpful in this case (reply: 1)
  177. primer design for subclone - (reply: 2)
  178. Mutagenesis and primer purity? - (reply: 4)
  179. PCR primer design, real time versus old school - (reply: 2)
  180. I have a problem with the M13 tailed primer method - M13 tailed primers-SSR (plants) (reply: 1)
  181. cDNA synthesis using random hexamer vs. oligo dt primers - (reply: 2)
  182. PCR primers with overhangs - (reply: 2)
  183. Primer designing - (reply: 1)
  184. Designation of PCR primers in ChIP experiment - (reply: 3)
  185. What is your favorite tool for primer design ? - (reply: 2)
  186. Primer express? - Do you use it? (reply: 2)
  187. Bad primer design ? How can I know ? - How could I know if a primer would recognize a particular sequence ? (reply: 2)
  188. What is the proper upper limit of false priming in real time? - (reply: 3)
  189. Enzyme sites on Primer - (reply: 3)
  190. gene-specific Primer for qRT-PCR - (reply: 2)
  191. Designing RT-RCR Primers according to other species' homology - Using conserved regions as a guide when mRNA isn't known in your a (reply: 1)
  192. primer design for PCR cloning - (reply: 1)
  193. need help to evaluate primer sets for SYBR green qPCR - (reply: 2)
  194. PCR primers artifact - (reply: 2)
  195. qPCR primer efficiancy - (reply: 2)
  196. Primer Express by ABI - (reply: 2)
  197. primer design for site-directed mutagenesis - (reply: 3)
  198. what affects primer specificity? - (reply: 2)
  199. Assembly PCR - Design of PCR program for primers w/low Tm? (reply: 2)
  200. Primers PCR - storage (reply: 5)
  201. MSP: M and U primers products - (reply: 1)
  202. Human Epigenome Project primers? - (reply: 2)
  203. Creating short dsDNA from primers for cloning - (reply: 2)
  204. Degenerate Primers - Help with Degenerate Primers (reply: 1)
  205. PCR: Can you get amplification if only one primer anneals? - Same size pcr product using 2 different primer sets (reply: 8)
  206. ready to use primers for BSP - (reply: 4)
  207. Are there any special rules for primer design? - (reply: 5)
  208. what's the difference between sequencing primer and cloning one. - confused (reply: 5)
  209. sequencing issue - Internal primers (reply: 9)
  210. GAPDH primer sequences for mRNA level in HeLa cells - (reply: 2)
  211. RT PCR Primer dimer tissue dependent SYBR - (reply: 1)
  212. FGF20 PRIMER - (reply: 2)
  213. qPCR for microRNAs - minimal primer length? (reply: 6)
  214. primer design for Syber Green - (reply: 1)
  215. primer sets to do pyrosequencing - (reply: 3)
  216. primer walking design tool - (reply: 1)
  217. optimizing PCR for low Tm primers - (reply: 1)
  218. primer design for MSP and BSP - primers must be in promoter region? (reply: 1)
  219. primers TM - (reply: 8)
  220. Degenerate Primers and dI - (reply: 5)
  221. primer dissolved in wrong buffer (1M Tris HCl) - what to do now?? (reply: 7)
  222. designing a primer - (reply: 5)
  223. preference for region of primer design for qRT-PCR - (reply: 1)
  224. using primers programms - (reply: 9)
  225. qPCR housekeepers - primers or probes? - (reply: 1)
  226. PCR Trouble Shooting - new primer, wrong dilution of primers, wrong band, and many more (reply: 15)
  227. freaky primers - (reply: 5)
  228. long range PCR primer design - (reply: 1)
  229. How do you design primers of PCR-STR used in Forensic Medicine - Urgent!!!! (reply: 2)
  230. what is CDS primer used as a control for QPCR in CHIP? - (reply: 2)
  231. Primer tag info - (reply: 1)
  232. How to get rid of primer dimers - (reply: 2)
  233. problems with PCR primers - (reply: 15)
  234. one nucleotide in the primer is wrong - (reply: 2)
  235. Primer Dilution. ...water or buffer? - (reply: 15)
  236. real time PCR using two sets of primers with different melt curves - (reply: 4)
  237. Howto: Maximum possible yield from a PCR? - What to optimize: Mgcl/primers/Polymerase/enhancers? (reply: 4)
  238. Introduce restriction sites with PCR primers - (reply: 2)
  239. primer flaw I'm missing? - (reply: 7)
  240. MSP Primer design -rules to choose primers and reasons - (reply: 2)
  241. How long we can keep Diluted Primer? - (reply: 5)
  242. Primer calculation - (reply: 3)
  243. PCR primer resuspend method? - (reply: 9)
  244. Testing newly designed primers prior to real time - (reply: 2)
  245. About Degeneracy of a degenerate primer~~ - (reply: 2)
  246. information regarding PCR using very long primers - (reply: 4)
  247. MSP primer design - understanding the basics - (reply: 2)
  248. Bisulfite: PCR condition or primer problem? - (reply: 13)
  249. Primer concentration and standard curve slope in "Relative Standard Curve M - (reply: 1)
  250. storage of primers - (reply: 3)
  251. Curious - different primer synthesis? - (reply: 4)
  252. too many hits on Blast for primers / microsatellite - (reply: 4)
  253. Sequencing PCR products with biotin-labelled primer - (reply: 1)
  254. sequencing new "unknown" plasmid - any good SV40 primers out there? (reply: 1)
  255. how to get DNA sequences starting from the primer - (reply: 4)
  256. Primer orientation, forward and reverse - (reply: 4)
  257. primers for bisulfite-modified DNA: general questions - (reply: 6)
  258. Negative control primer for ChIP - (reply: 3)
  259. primers for promoter amplification - (reply: 1)
  260. RT-PCR primer designing - (reply: 6)
  261. PCR with overlapping primers - (reply: 1)
  262. Free program for primer designing? - Need to check promoter sequence for possible primer binding sites (reply: 3)
  263. Can any one suggest a company who can design a primer pair - dnaK PCR work (reply: 1)
  264. My primers don't work with Chip - (reply: 1)
  265. LNA Primers design - (reply: 1)
  266. qPCR using random primers - (reply: 4)
  267. designing primers for cloning - designing primers for cloning (reply: 1)
  268. Lambda DNA nanowires, Restriction sites and thiolated primers - (reply: 1)
  269. 1. ABI Power SYBR® Green primer efficiency? - (reply: 1)
  270. Amplifying a 1700bp fragment using a mismatched primer pair - (reply: 2)
  271. Identification by 16S rDNA - using short primer (reply: 2)
  272. Degenerate Primers - (reply: 12)
  273. restriction enzymes primers - (reply: 5)
  274. Plasmid DNA Sequencing with Universal Primers - (reply: 3)
  275. Primer Efficiency (how to change the slope) - (reply: 5)
  276. Optimisation of dCAPS markers (degenerate primers) - problems with detection of heterozygotes (reply: 1)
  277. Ever heard not of Prime Dimers but Primer Multimers? - (reply: 1)
  278. Possible mutations in primers? - (reply: 4)
  279. How many of us request for PAGE purification of primers more than 30 mers in len - PAGE purified primers (reply: 5)
  280. Need to confirm PCR primers are bad - (reply: 5)
  281. cloning primers, TM, and specificity - (reply: 4)
  282. Help! Primer Dimers… - (reply: 3)
  283. Allele Specific PCR Primer Design - (reply: 1)
  284. Very low PCR primer concentrations? - (reply: 4)
  285. Question about BSP primer design? - What is the complementary strand for forward primer? (reply: 3)
  286. Primer sets for Bisulfite-modified and unmodified DNA - (reply: 6)
  287. Designing forward primer incorporating Nde1 cut site - Tips on cloning with Nde1? (reply: 2)
  288. Primer Design for Gene Inserted Counterclockwise into Plasmid - Please help...very basic question for a mol. biologist (reply: 1)
  289. PCR Primer again - (reply: 1)
  290. False priming in MSP - (reply: 4)
  291. Polymerase Choice Troubles - my primer melting temp is so low (~30 C) (reply: 4)
  292. Can primers go bad and give a smear in PCR? - (reply: 12)
  293. primer Tm - (reply: 10)
  294. QuickChange primers self-made - (reply: 4)
  295. oligos as synthetic "miRNA" for quantification? - would an oligo / primer work in AB RT kit? (reply: 2)
  296. PCR Conditions for primers with GC Clamp and without GC Clamp - (reply: 3)
  297. Non specific amplification - How to check if the primers give non specific amplification or not (reply: 4)
  298. digoxygenin-labelled primer - (reply: 3)
  299. How to import GeBank files into Primer Express 2.0 - (reply: 1)
  300. freezing of primers - (reply: 2)
  301. Primers with RE sites - (reply: 2)
  302. Primer dilution and calculation - (reply: 4)
  303. what can i do with this pcr? - amplification of cDNA , with primers tm 50 and 67 (reply: 7)
  304. Designing primer for Banana mild mosaic virus - (reply: 3)
  305. attaching t7 promoter sequence to template primer - (reply: 1)
  306. primer for real-time and reverse transcriptase PCR - (reply: 1)
  307. PRIMER 3 PROGRAMME - (reply: 8)
  308. Bisulfte PCR and sequencing PCR and Primer Notes - Tips and hints on PCR for bisulfite converted DNA and bisulfite primer (reply: 33)
  309. primers stopped working - (reply: 3)
  310. humanized antibody - how to design primers for CDRs - humanized antibody (reply: 1)
  311. Primers going bad - what's the best storage/usage conditions? (reply: 4)
  312. 5' Primer modification - Tm-effect ?? (reply: 1)
  313. Designing PCR Primers - (reply: 3)
  314. sequencing - which primer should I trust? (reply: 4)
  315. running a gel with primers - (reply: 2)
  316. Help- degenerate primer design - (reply: 4)
  317. Will this primer work? - (reply: 8)
  318. Use of W primers to detect efficiency of bisulfite reactions? - (reply: 5)
  319. What are the most important parameters in PCR primer design? - Tm difference, GC clamp, GC%, primer-primer complementarity, self-complementarit (reply: 9)
  320. Is a Kazark sequence needed in a upper primer? - (reply: 17)
  321. Primers and enzymes not compatible - How can I fix this? (reply: 2)
  322. cytokines primers - (reply: 1)
  323. Single primer PCR - (reply: 3)
  324. Inverse PCR primers design - (reply: 4)
  325. Enzyme Site in reverse Primer - (reply: 3)
  326. general pcr/primer question - (reply: 1)
  327. for the PCR primer what is better the hairpin or the self annealing? - (reply: 9)
  328. primers in PCR - primer removed or not (reply: 5)
  329. Is this kind of primer OK? - (reply: 2)
  330. different primer efficiency using cDNA and plasmid/PCR product - (reply: 4)
  331. RNA in cDNA - Can I use the same PCR primers for the production of cDNA? (reply: 3)
  332. Primers for BS treated DNA - (reply: 5)
  333. Primer Design software - (reply: 8)
  334. concentration of primers - (reply: 5)
  335. Primer design - (reply: 4)
  336. forward & reverse primers - (reply: 3)
  337. Fusion protein questions - Botched deletion primer (reply: 2)
  338. Primers & probes of Applied Biosystems for real time - (reply: 2)
  339. Primer walking - (reply: 2)
  340. Methyl Primer Express from ABI - (reply: 2)
  341. Primer Optimiztion - can this be done in water (reply: 8)
  342. design primer - (reply: 5)
  343. increasing sensitivity and reducing primer dimmer - (reply: 11)
  344. can Primers for RT-PCR be used Real-Time PCR directly? - (reply: 2)
  345. Can DNA polimerase amplify primers itself? - need help please!! (reply: 7)
  346. primer design questions for confused - (reply: 2)
  347. Silly Question about RT-PCR/Primer design - (reply: 2)
  348. primer selection for RT prior to real time - an insiders hint!!! (reply: 1)
  349. Which primer concentration for RT with very low RNA amounts? - (reply: 1)
  350. primer concentration - (reply: 7)
  351. restriction site in primers urgent - (reply: 1)
  352. RT-PCR primer design: Over introns and exons? - (reply: 2)
  353. primer design problems - (reply: 4)
  354. SYBR VS TAqman- What is the difference other than PRimers? - TAqman works and SYBR doesnt (reply: 1)
  355. Which primers are better for colony PCR? - (reply: 4)
  356. pcr yield and primer concentration - (reply: 5)
  357. RT-PCR - not qPCR! urgent please - how to choose primers (reply: 3)
  358. Primer design with restriction sites - (reply: 3)
  359. Why there is use of only a single primer in case RAPD amplification - (reply: 1)
  360. differences between oligo and random primers - (reply: 2)
  361. RT-PCR primers - (reply: 3)
  362. Any primer design programs...? - (reply: 8)
  363. Problem SNP that genotypes differently using different primers? - (reply: 2)
  364. Cutting out of a T vector - Cutting sites in primers vs sites in vector (reply: 3)
  365. PCR problems, huge artifacts, no product - involves 16S primers (reply: 8)
  366. primer : shifting peak - (reply: 4)
  367. Primer design for BSP - (reply: 12)
  368. Designing primers and hybridization probes - (reply: 1)
  369. Random Primers vs Oligo(dT) primers? - (reply: 3)
  370. ChIP primer for promoter region of GAPDH - for taqman analysis (reply: 2)
  371. 5' phosphorylated primers - (reply: 1)
  372. Methylation specific primer designing - (reply: 1)
  373. primer design with intentional mismatch - (reply: 3)
  374. Primer Extension Assay versus RACE-PCR - Protocol (reply: 1)
  375. please tell me more about primers with restriction site! - (reply: 4)
  376. RT-PCR primer design - (reply: 1)
  377. Primers work for regulary PCR but not real-time PCR - why? (reply: 8)
  378. how to check primers - (reply: 4)
  379. How to design primer for unknown DNA sequence? - (reply: 9)
  380. TM for primers with restriction secuence tail. - Suggestion (reply: 3)
  381. can primers age? - (reply: 1)
  382. How to determine PCR Annealing temperature for primers with different Tm - (reply: 7)
  383. poor random priming for Northern Blot probes - (reply: 1)
  384. PCR with 75 mer forward primer - (reply: 4)
  385. half life of primers - (reply: 2)
  386. about the primers in MSP - (reply: 5)
  387. What annealing temperature is better for my primers? - (reply: 7)
  388. primer dimer in pcr product - cloning for a gene. analyzing pcr product shows primer dimer (reply: 8)
  389. primer concentration (calculation problem) - (reply: 5)
  390. PCR, labelled vs unlabelled primers - (reply: 4)
  391. How to add restriction enzyme sites to the primer - (reply: 6)
  392. Using pentadecamer primers for reverse transcription - Better than hexamers, oligo d(T)? (reply: 7)
  393. primer:cross-dimer - How to remove the cross dimer? - (reply: 3)
  394. Bands appear poor/not at all and do not appear consistantly & primer bands a - (reply: 2)
  395. can I detect colony for the insert with the cloning primers? - or clamp sequence will interfere? (reply: 4)
  396. The weirdest primer ever - (reply: 7)
  397. oligo(dT) plus random primers in RT - (reply: 4)
  398. no RT controls - can I run on separate plate and for just one primer set? (reply: 1)
  399. primer calculation - concentration (reply: 2)
  400. What happens to PRIMERS at 72 C ? - (reply: 5)
  401. 0,2uM is 100ng of a 18-mer primer - (reply: 8)
  402. Melting Curve giving flutating Ct values - Advice on primer design? (reply: 9)
  403. checking colonies by vector primers.... - (reply: 7)
  404. Direct BSP using tag-modified primers - (reply: 2)
  405. adding restriction sites to degenerate primers - (reply: 9)
  406. Mutagenesis using 4 sets of primer - (reply: 6)
  407. cloning primer and tag questions - first try (reply: 2)
  408. PCR analysis of clones - Can a gene specific primer pair amplify a fragment in the wrong direction? (reply: 1)
  409. Amplification of gene (CHH,MIH,GIH) from prawns. - Amplification of gene using primers designed on the basis of cDNA sequences (reply: 1)
  410. pro-PCR with only one primer... - to check directionality. (reply: 4)
  411. Two design programs give very different primer Tm values - RT-PCR (reply: 5)
  412. cDNA synthesis primer - (reply: 3)
  413. Primer with tag: Which is the real concentration? - (reply: 3)
  414. antigen primers - (reply: 1)
  415. How to design PCR primers in ChIP assay - (reply: 2)
  416. Annealing vs melting Ts for real time PCR primers - (reply: 1)
  417. relative quantification, primer pair problem - (reply: 1)
  418. Calculating Tm for bisulfite primers? - (reply: 3)
  419. Designing species specific primers for E.coli - please help? (reply: 3)
  420. Using primers meant for DIG probe as PCR diagnostic - (reply: 1)
  421. Using primers meant for DIG probe as PCR diagnostic - (reply: 3)
  422. Need for Nested PCR - Primers Designed with MethPrimer - (reply: 6)
  423. Topo Zero Blunt Vector by Promega Amplification of insert with M13 - Amplification of insert with M13 primers (reply: 1)
  424. Primer for 5' RACE - (reply: 2)
  425. Stick T7 on custom primer for sequencing? - (reply: 2)
  426. ABI have released MethylPrimer Express! - A new primer design program for DNA methylation....For Free!!! (reply: 45)
  427. MSP primer design help - (reply: 5)
  428. PCR using long primers and two overlapping templates - PCR using long primers and two overlapping templates (reply: 2)
  429. Primers for nested-PCR - (reply: 1)
  430. PCR primers with high Tm - (reply: 6)
  431. how to choose best annealing temp. for primers? - (reply: 6)
  432. Primer Dimer problem- Different perspective - COuld there be a treatment effect (reply: 4)
  433. PCR using a long (100nucleotides) and a short (20 nucleotides) primer. Problem: - (reply: 7)
  434. making primers for similar protein sequences - making primers for similar protein sequences (reply: 4)
  435. BSP and MSP Primers - (reply: 2)
  436. Bisulfite sequencing primer - (reply: 4)
  437. MSP Primer Sets (Herman Papers) - (reply: 1)
  438. random primers for qPCR - (reply: 3)
  439. QPCR, where to start? - optimizing primer and template (reply: 5)
  440. difficulty in Primer walking - (reply: 2)
  441. PCR primers Tm higher than extension T - (reply: 8)
  442. Primer for sequencing - need help!!!!!!!!!! (reply: 6)
  443. Primer Design - (reply: 2)
  444. Primer design invert pcr - (reply: 5)
  445. cloning two fragments with deletion in between - PCR primer design (reply: 3)
  446. Vector construction - Can I use long PCR primers (60bp) and get good PCR poduct??? (reply: 4)
  447. designing primers for Real Time - (reply: 1)
  448. Stem loop RT-PCR for miRNA quantification - how can I design the stem loop primer? (reply: 2)
  449. Methods for primer reconstitution - (reply: 23)
  450. BSP Primer placement over KNOWN unmethylated CpG's - Can this be done? (reply: 3)
  451. no result with pcr. is it the primer?/ - no amplification in pcr even after starting from the beginning (reply: 6)
  452. dimers of dna (not primers) following digest or pcr? - (reply: 11)
  453. can we add the terminaton codon in the 3' primer? - (reply: 1)
  454. Question about Realtime PCR primer concentration.... - (reply: 5)
  455. designing primer for methylation - (reply: 21)
  456. plasmid amplification - exons and primers (reply: 1)
  457. Primer dimers?Contaminations? - Unexpected bands! (reply: 5)
  458. reverse primer design with restriction site - (reply: 6)
  459. Reverse Transcription with oligo dT or hexamer primers? - What do you prefer and why? (reply: 3)
  460. question in 5' RACE and primer extesion - (reply: 8)
  461. Primer concentration ? - (reply: 2)
  462. PCR product dimer (not primer dimer!) - PCR (reply: 2)
  463. Q-PCR questions: Primer design and melting curve - (reply: 13)
  464. from which strand to derive BSP primers? - (reply: 5)
  465. PCR - free primer designation program (reply: 2)
  466. Primer Annealing for PCR - (reply: 4)
  467. cDNA library screening with 5' specific primer - (reply: 1)
  468. Site-Directed Mutagenesis- Doubt - Overlapping primers (reply: 3)
  469. Both the PCR primers in a vial. - Did anybody mix the diluted primers in single tube for use? (reply: 7)
  470. How to design primer for sequencing unknown DNA - (reply: 5)
  471. PCR - forward, reverse primers - is the reverse primer the "reverse complement" of the sense strand (reply: 2)
  472. need helps on probe design--human ip10 primers and probe. - (reply: 2)
  473. how to determin the Annealing Temp of ristriction sites modified primer? - help me ! (reply: 26)
  474. Sequencing Primers - (reply: 4)
  475. 5' RACE - gene specific primer - (reply: 1)
  476. RT PCR on ALM patients - help for designing primers for detect PML RAR (reply: 2)
  477. About PCR primers' concentration - (reply: 2)
  478. Primer design with incomplete sequence - (reply: 2)
  479. Annealing temperatures for long primers? - PCR reaction (reply: 6)
  480. PCR primers from amino acids? - (reply: 1)
  481. Primer dimers - (reply: 1)
  482. primers design for mutagenesis of two nearby sites - mutations (reply: 2)
  483. design spesific primer of avr, hrp, toxin and falgella gene - (reply: 3)
  484. Intensive primer dimers - (reply: 2)
  485. PCR using different primers to generate same DNA sequence - explanation please :) (reply: 2)
  486. PCR is positive with one set of primers, negative with another. - (reply: 4)
  487. Do you synthsize your own oligo(dT) primer? - (reply: 2)
  488. Trouble with primers of BSP - (reply: 3)
  489. degenerate primer design - (reply: 4)
  490. potassium acetate in primer solution - I made a mess but need to recover (reply: 6)
  491. 16S rRNA primers for E. coli - (reply: 2)
  492. primer design - how to design primers (reply: 2)
  493. Multiplex Primers - Primer primer interaction (reply: 1)
  494. Why primers for bisulfite converted DNA amplify unconverted DNA? - (reply: 3)
  495. RT-PCR primer design at 3'-UTR or ORF? - (reply: 2)
  496. Reverse transcription of poly-A tailed miRNAs using anchored Oligo dT primers?&# - (reply: 6)
  497. Primer design for environmental samples - Align sequences first? (reply: 3)
  498. southern hybridizaion with 5' end labelled primer - (reply: 1)
  499. Programs for designing Primers - (reply: 6)
  500. Disadvantages of using oligo-dT primers - (reply: 1)
  501. How to eliminate primer dimer in MSP - (reply: 20)
  502. do primers and dNTPs alter digestion efficiency? - (reply: 4)
  503. Sequencing primer dilution - (reply: 2)
  504. PCR with degenerate primer - (reply: 6)
  505. 1.3kb PCR only shows primer dimer - (reply: 2)
  506. RT-PCR primers for E. coli LacZ gene - (reply: 3)
  507. water instead of TE buffer to dissolved primers - (reply: 6)
  508. primer reconstitution - (reply: 2)
  509. Question about PCR primer with degenerate base at 3' end - (reply: 2)
  510. Primer for 4 genes sharing short consensus sequence - (reply: 2)
  511. what is the best pair of sequencing primer for puc 18 - (reply: 1)
  512. Looking for online primer design for SNaPshot - (reply: 1)
  513. pCI sequencing primer - (reply: 1)
  514. PCR primers: hairpin and dimers? - what can I do to avoid this? (reply: 4)
  515. Reconstitute primers - (reply: 4)
  516. multiplex PCR for three sets of primers - (reply: 1)
  517. inner primer for BSP PCR? - (reply: 6)
  518. How many mismatches in point mutation primers? - (reply: 3)
  519. Software available for Multiplex PCR primer designing - (reply: 3)
  520. single primer PCR - use a single primer to amplify regions between inverted repeats (reply: 1)
  521. PRIMER design - primer dimers (reply: 1)
  522. Mouse MSP primers - (reply: 11)
  523. PCR primer: 2 restriction sites? - (reply: 4)
  524. Primer Dimer problems in real-time PCR - (reply: 14)
  525. primer orientation - (reply: 1)
  526. Degenerate primers for nested PCR? - (reply: 1)
  527. so lost! stupid quenstion about primers.... - designing the primers (reply: 2)
  528. Reliable ChIP primers for Sp1? - (reply: 2)
  529. house-keeping primers for Cos7 cells - (reply: 4)
  530. increased primer dimer with template - (reply: 2)
  531. designing the sequencing primer - (reply: 2)
  532. free software to examine if two primers will make dimers - (reply: 3)
  533. primer efficiency in real time PCR - need for a method explanasion (reply: 1)
  534. Testing primers - (reply: 3)
  535. design of PRC primers of full ORF - (reply: 3)
  536. Two round MSP with the same primer - (reply: 1)
  537. primer dimers - (reply: 4)
  538. Designing a Primer within an exon - Consesus required - (reply: 2)
  539. Nested primer PCR - (reply: 5)
  540. difference between primer extension and RACE - (reply: 6)
  541. designing QRT-PCR Primers - (reply: 11)
  542. Primer - (reply: 1)
  543. Optimizing primers for SYBR green qPCR? - Optimizing primers for SYBR green qPCR? (reply: 7)
  544. rat 18s rRNA primer - designing primers (reply: 1)
  545. Which MSP & BGS primer design tool do you "swear" by? - (reply: 9)
  546. Designing primers for multiplex - BLASTing lots of primers (reply: 3)
  547. Why random primers for RT-qPCR - (reply: 1)
  548. Primer design question - Use of primer 3 (reply: 3)
  549. Primer extension - yeast tRNA as a negative control (reply: 3)
  550. RT-PCR Primer design - which region? - (reply: 2)
  551. primer design for MSP - How many CpG in the primer? (reply: 2)
  552. No PCR products in MSP with MethPrimer designed primers - (reply: 2)
  553. problems with mix of MSP's primers - (reply: 2)
  554. design primer for PCR.. - (reply: 3)
  555. From MSP primer to gene - how to recalculate original DNA sequence? (reply: 4)
  556. Design primer - (reply: 2)
  557. MSP primers not working - (reply: 1)
  558. extra sequence in primers - (reply: 4)
  559. Primer Programm for Sequencing - (reply: 3)
  560. calculating Tm of primers - (reply: 8)
  561. Primer extension problem - there are too many bands!! (reply: 1)
  562. How to design random primers - (reply: 2)
  563. design pcr primers - (reply: 3)
  564. PCR primers for Mycoplasma detection? - Publication gives many, which to choose? (reply: 1)
  565. Does it help to add antisense primer first for bisulfite PCR? - (reply: 2)
  566. primer design problem - (reply: 1)
  567. Bisulfite treated DNA specific primer set critique? - (reply: 18)
  568. 60 bp PCR and primer dimers - (reply: 6)
  569. No product/primer dimers after BSP - (reply: 1)
  570. Primer Express Software - Probe Design (reply: 8)
  571. Primer design:Is it ok to omit extra bases after RE site? - (reply: 4)
  572. Problems with methylation analysis!!! - Primers crossreaction etc. (reply: 3)
  573. primers for SoE - (reply: 1)
  574. designing flanking primers around msp primers - (reply: 1)
  575. Primer for BSP - (reply: 1)
  576. BSP cycles and primers - Is it OK to use the same primer set but with more (reply: 6)
  577. primer hairpins and self complementation - online primer checker.. (reply: 2)
  578. primer does not bind - help (reply: 2)
  579. reconstitute primer - (reply: 4)
  580. SOS call: ChIP assay in Huh7 cells - How many cells? And primer position? (reply: 3)
  581. Online primer design - (reply: 7)
  582. RT-PCR inconsistent primer efficiencies - RT-PCR (reply: 3)
  583. Unmethylated primer control - (reply: 4)
  584. is it effective to use a primer of SD rat to Wistar rat in PCR - help (reply: 2)
  585. Custom Primers - (reply: 2)
  586. Primers for RT reaction - (reply: 6)
  587. Primer Tm - How low can you go? - (reply: 3)
  588. How to prepare primer solution - (reply: 7)
  589. Primers design: how to check for mispriming? - (reply: 2)
  590. primer storage - (reply: 7)
  591. Pls check my bisulphite sequencing primers - (reply: 12)
  592. Help: Check my MSP primers - is my MSP primer correct??? (reply: 1)
  593. Bad Primer - (reply: 2)
  594. reducing primer dimer - (reply: 1)
  595. Calculating correct melting temperature for primers - (reply: 2)
  596. primer melting temperature - (reply: 2)
  597. Bisulfite Sequencing Primer Design - (reply: 3)
  598. end of primer - (reply: 2)
  599. degenerate primer desgining - degenerate primer desgining (reply: 5)
  600. PCR primer dimer? - (reply: 7)
  601. checking primer - checking primer (reply: 5)
  602. PCR primer Tm and concentration question - (reply: 3)
  603. How to avoid secondary structure in primer - (reply: 2)
  604. cloning primer orientation - orientation and complement of primers in a cloning (reply: 3)
  605. Random hexamer vs oligo dT vs gene specific primer for RT - which do you use most? (reply: 5)
  606. primer design - checking off-target site - (reply: 5)
  607. What is the distance between a forward and reverse primer - (reply: 1)
  608. Which RT primer is better for qPCR - (reply: 2)
  609. PCR; Perhaps someone can explain? - PCR; Primer (reply: 3)
  610. Why different amounts of forward and reverese primers in RT-QPCR - (reply: 2)
  611. Degenerate primers - (reply: 1)
  612. primer optimization - (reply: 1)
  613. primers for Q-PCR - (reply: 2)
  614. multiplex PCR - template and primer concentration (reply: 2)
  615. Primers and templates - Primer and template concentrations (reply: 2)
  616. Real Time qPCR - qPCR SYBR greenI primer design (reply: 5)
  617. Primers for species identification - Know any good? (reply: 6)
  618. The perfect number of CpGs in MSP primer - (reply: 8)
  619. Primer Express 2.0 - crashes (reply: 1)
  620. Primers - (reply: 1)
  621. How to measure the concentration of primers? - (reply: 2)
  622. Probes or primers that span introns - (reply: 6)
  623. Tm for my PCR product (not Tm for the primers!) - (reply: 3)
  624. what primers specific for B.stearothermophilus?Help pls. - (reply: 5)
  625. help regarding primers reconstitution - (reply: 4)
  626. problem with my PCR - primer dimer. - (reply: 1)
  627. PCR - Long PCR with short primers (reply: 1)
  628. how long a primer should I use? - (reply: 1)
  629. identifying exon and intron for primer design - (reply: 2)
  630. what is the "tailed primer and taq primer"? - (reply: 2)
  631. Designing methylation primers on long CpG island - (reply: 14)
  632. Difficutly go separate product from primer dimers - Methylation specific PCR (reply: 1)
  633. real time PCR primer for methylation - (reply: 2)
  634. designing gene-specific primer for cDNA synthesis - (reply: 5)
  635. Primer design for unknown gene - (reply: 4)
  636. How to design primers for CpG, CpNpG, CpHpH methylation - Bisulfite Sequencing PCR (reply: 2)
  637. Designing primers from amino acid sequence - cloning problem (reply: 3)
  638. software to find restriction site and TM for primer - (reply: 7)
  639. sense/antisense specific pcr - design of sense/antisense specific pcr primers (reply: 1)
  640. product size for real-time PCR primer - (reply: 3)
  641. GC content of PCR primers - (reply: 3)
  642. real time PCR primers - (reply: 2)
  643. mC not in CpG? Primer selection - (reply: 1)
  644. cpgware primer design, does it work - (reply: 4)
  645. Who will give me a software to design primer for PCR? - (reply: 3)
  646. PCR Primer Design with restriction sites - (reply: 1)
  647. primers with different Tms - PCR amplification using primers with different Tms (reply: 9)
  648. Sequence input for bisulfite PCR primer design - (reply: 1)
  649. Primer design for MSP queries - (reply: 3)
  650. Database software to catalogue / archive primers - Database software to catalogue / archive primers (reply: 1)
  651. Real Time PCR Annealing vs. Melting - Relation of anneal temp rxn to melt temp primer (reply: 2)
  652. primer design for bisulphite treated DNA - (reply: 1)
  653. primer dimers - what exactly are primer dimers (reply: 2)
  654. primer design: end-point vs. RT - (reply: 1)
  655. Primers for unmethylated DNA work too well! - (reply: 2)
  656. software to check the primer dimer - (reply: 3)
  657. Control for primer extension - (reply: 2)
  658. BSP and MSP primer check - (reply: 2)
  659. Using a big primer in PCR - (reply: 4)
  660. How to get rid of PCR primer dimer - (reply: 65)
  661. designed primer more powerful? - (reply: 1)
  662. RT-PCR primer design - exon-exon primer design (reply: 5)
  663. Primer design - PCR for cloning (reply: 6)
  664. availabre primers for MSP - (reply: 1)
  665. 3' end of forward primer mismatch - (reply: 5)
  666. RACE - specific primers in first strand synthesis (reply: 4)
  667. qPCR Primer Design and Genomic DNA Amplification - (reply: 1)
  668. Primer Designer Online - Pipleline use of designer (reply: 1)
  669. If the primer is dimer,it's ok? - (reply: 5)
  670. methylation primers - problems with primers (reply: 1)
  671. Multiplex PCR primer designing - (reply: 4)
  672. real-time PCR - How to design primers (reply: 2)
  673. BiPolar Clamps on PCR primers for TGGE - (reply: 1)
  674. PCR primers - PCR primers for RT-PCR (reply: 1)
  675. multiplex primers - (reply: 6)
  676. Primers: advice? - (reply: 1)
  677. primer3 - select primer in 3' end (reply: 3)
  678. bisulfite pcr primers - (reply: 1)
  679. The PCR Suite - a set of new primer design tools - (reply: 1)
  680. RT-PCR primer design guide - How to check gene structure and design the primer? (reply: 15)
  681. Primer dimers - (reply: 1)
  682. Double strand cDNA sysnthesis - Using random primers (reply: 1)
  683. degenerate primer - optimizing degenerate primers (reply: 1)
  684. Need suggestions for degenerate primers - (reply: 2)
  685. Primer extension - (reply: 1)
  686. Primer design - Primer design for RT-PCR (reply: 1)
  687. I am afraid I got wrong Primer from company - (reply: 2)
  688. Primer contamination - (reply: 2)
  689. degenerated primer for nested RT PCR - (reply: 5)
  690. questions about degenerate primer PCR - (reply: 2)
  691. design a primer - how to determind a specific primer in a complete gonome? (reply: 1)
  692. How to find/check the highly conservative sequence - RT-PCR primers (reply: 2)
  693. Scorpion Primers? - Synthese of Scorpion Primer (reply: 1)
  694. primer purification - search method for primer purifying (reply: 3)
  695. primer design tools - primer designing (reply: 28)
  696. Software for MSP (methylation-specific PCR) primer design - (reply: 3)
  697. primer design - Deinococcus (reply: 1)