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Primer Related Discussions
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ITP substitutes... what? - can I add ITP to my degenerated primers to lower the degeneracy? (reply: 2)
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need help...how to compare mouse vs human sequences for primer design - (reply: 2)
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PCR problem (great different of Tm in primers) - (reply: 3)
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Create an artificial restriction site for a endonuclease and 53bp long primer? - (reply: 2)
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Tm of PCR primers: one 68, the other 58. Impossible to succeed? - (reply: 3)
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Primer design for PCR following ChIP - (reply: 3)
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How to name primers? - Any official nomenclature systems to name primers? (reply: 4)
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Primer dimers!? HELP - (reply: 8)
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MSP primer does not work - (reply: 5)
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How primers can be measured? - (reply: 6)
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RE sites on the primer. - How can I check for restriction sites on a primer sequence? (reply: 2)
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What are normalisation primers for the rt-PCR? - Im going to order the mirVana rt-PCR kit for miRNAs (reply: 1)
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RNA contamination in primers - (reply: 2)
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how 2 design rev.transPCR primer? - need functioning protocol.... (reply: 1)
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methylnick.please help me with MSP primer designing! - (reply: 3)
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concentration of primers - (reply: 1)
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About DAPK1 MSP primer design. - About MSP primer design. (reply: 4)
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Primer design from mRNA or DNA - Confused about what sequence to use for design of GSP and pPCR primers (reply: 2)
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deisgning primers with restriction site - (reply: 7)
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Questions about primer design for BSP in plant - shouldn't we take the mC in CHG CHH context into consideration? (reply: 3)
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cDNA primer help - (reply: 1)
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cDNA and primer Design Help - (reply: 6)
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Bisulfite sequencing reproducibility - overlapping PCR amp primers give varying results (reply: 2)
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Primer design program for Methylight - (reply: 3)
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primer design software - primes for full lenth gene (reply: 3)
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Please help me design MSP primer! - (reply: 4)
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What grade of primers for real-time TaqMan PCR assays? - (reply: 1)
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Tm of the primers - company sends primers with lower Tm than expected (reply: 3)
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RT-PCR primer design - RT-PCR primer design...need ur help (reply: 3)
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RT-PCR primer design - RT-PCR primer design...need ur help (reply: 1)
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primer sequence - reverse primer sequence (reply: 3)
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cDNA and additional bands when run on gel - I get a band o fthe same Mw regardless of the primers I use (reply: 2)
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How to synthesize your own oligo-dT primer - (reply: 4)
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Designing Primers - (reply: 1)
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mrna detection with sequence specific primers - (reply: 2)
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Standard sequencing primers - how to find which sequencing primers bind to my DNA? (reply: 2)
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internal control primers for plants - (reply: 14)
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qRT-PCR primer design - (reply: 1)
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Where do you get your PCR primers from? - (reply: 3)
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PCR primers + large mRNA amplification - (reply: 5)
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PCR Primers for introducing tag - (reply: 5)
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help design primer - (reply: 6)
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BLAST Help , Designing primers... - (reply: 5)
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Different efficiency in different samples but the same primer - (reply: 1)
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Primer design and degeneracy - Are synthesized sets identical? (reply: 2)
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primers amplify additional band (!) - (reply: 1)
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resuspended primers in water - is this a problem?! (reply: 5)
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Designing the primers for qRT PCR - Query - (reply: 14)
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primers sequence - (reply: 2)
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primer designing - (reply: 1)
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How to design PCR primer to amplify a very long sequence(about 9.7kb) - I want to amplify cDNA genome of HCV. It's about 9.7kb long (reply: 2)
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Primer Design for nucleotide sequence - (reply: 1)
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Primer dimer problem in MSP - (reply: 2)
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Attaching DNA bases - Creating primers and plasmids? (reply: 3)
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Primer doubts - (reply: 2)
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Why the addition of restriction sites to primers? - (reply: 3)
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Primer desing for transcript variant - (reply: 2)
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Primer analysis sites - Any suggestions? (reply: 3)
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RAPD and viruses - RAPD using very very short primers (reply: 4)
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please help me to be sure from design primer have it - (reply: 3)
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resuspend Primers in TE or in water? - (reply: 7)
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manual primer desgining - (reply: 2)
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BLAST search of primer sequence - (reply: 3)
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newbie question on Primer design for MSP - (reply: 2)
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Oligo dT primer - oligo dT primer with non-T priming 3´ or with 5´ (reply: 3)
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Confirming published methylation primers - (reply: 1)
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Help designing primers; also help with viewing GenBank - (reply: 3)
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Primer design - How to - am a novice ? (reply: 6)
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Primer optimization (with probes) - Urges... (reply: 2)
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SNP - How to design the SNP primer (reply: 1)
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Choosing Primer: random or Olig-dt ? - (reply: 2)
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how to synthesize the pcr primers - (reply: 3)
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designing degenerate primer - (reply: 2)
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incorporation of restriction site in Primers - Help to design primer to clone gene in frame with fluorescent tag (reply: 2)
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how to design pcr primers - (reply: 7)
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RT-PCR primer design and in silico PCR - (reply: 4)
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Primer extension and sequencing from total RNA - (reply: 1)
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primer design - over expression (reply: 1)
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PCR bias with BSP primers - (reply: 1)
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Primer design and tolerances - (reply: 3)
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primer design - (reply: 2)
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help with degenerate primer design - (reply: 1)
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PCR: Big Primers = Big Problems (for me) - I'm really not sure what's going on in those poor little tubes... (reply: 2)
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mutations in primer sequence - (reply: 2)
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Nested primers - (reply: 5)
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Primer design - Primer design for PCR with TE sites (reply: 2)
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Reverse transcriptation for qPCR with some specific primers. Should I use primer - (reply: 2)
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primer conditions for expression work - (reply: 5)
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Question about primer design targeting GC-rich region - (reply: 2)
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promoter pcr using primers with restriction sites giving trouble - (reply: 7)
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how to optimize primer and cDNA concentration for real-time - (reply: 2)
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primer Question pVax1 RE / Kozak - (reply: 1)
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Help -designing primers for real time RT-PCR - (reply: 1)
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Primer design for SNP detection - (reply: 2)
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Bad primer efficiency during multiplex qPCR - (reply: 1)
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max primer length - 85 nt too long? - (reply: 5)
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different transcription from 5' UTR than 3' UTR primers - (reply: 8)
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recommended primers for caspase 8 & 9 - human (reply: 4)
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UBC primer PCR - (reply: 1)
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Optimising bisulphite PCR primers - (reply: 4)
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Primer design help - secondary structures - Please help me troubleshoot? (reply: 12)
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Determine specific primers and transgene copy number by PCR - (reply: 1)
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optimization of PCR conditions - to know that primers are specific and detect copy number (reply: 1)
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Any Universal Primer for 3' of cDNAs of all mRNAs? - (reply: 2)
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better primer for cDNA synthesis in real time pcr - (reply: 1)
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can you judge a 22bp primer based on the 3' 10bp? - (reply: 6)
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Getting primer dimers in RT-PCR on and off? - (reply: 2)
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conc of cDNA to be used - PCR to test primers (reply: 5)
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Primer for all HPV - (reply: 4)
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please help me check BSP primers. - (reply: 6)
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Lengthy primers - (reply: 11)
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primer design - how far apart Tms could be? (reply: 4)
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design primers - (reply: 3)
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a problem about the Tm of the BSP primer - (reply: 2)
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Primers distinguishing splicing alternatives - (reply: 1)
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primer design from mRNA - (reply: 12)
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who can give me a hand to check the BSP primer? - (reply: 5)
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Please help me design BSP primer. - (reply: 14)
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Primer design: how do I add a restriction site to my primer? - (reply: 2)
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Storing primers...together? - (reply: 3)
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Genomic DNA fragment labeling by random priming - (reply: 1)
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different length for primers amplifying - (reply: 2)
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primer dilution - primer dilution from picomoles to micromolar (reply: 2)
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Primer concentration is quantitative Real-time PCR - (reply: 1)
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primer dimer annealing temperature - (reply: 2)
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design primers with custom end - To clone my gene into the MCS as fusions to the GFP tag (reply: 5)
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TetR primer - (reply: 1)
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some primer dilution calculations - (reply: 7)
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primer tm with mismatch? - (reply: 6)
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TOPO primer choice for sequencing - (reply: 3)
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Can do reverse transcription by gene-specific primers? - (reply: 3)
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Anchored oligo dT and Thermostable reverse transcriptase - temperature compatibility between RT and oligo dT priming (reply: 8)
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primer design with RE sites - (reply: 4)
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primer desighning with RE sites - (reply: 5)
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Primer design (long transcripts) - (reply: 2)
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Primers for Real time PCR - Really need some advice (reply: 1)
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Stem-loop RT primers: how to anneal? - (reply: 1)
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second strand cDNA synth without primer? - (reply: 2)
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do you put two pairs primers in one tube when you do site mutations? - (reply: 2)
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General question about restriction sequences in PCR primers - (reply: 3)
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GAPDH primer sequence - cloning of huGAPDH gene (reply: 2)
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chip assay primers - chip assay primers (reply: 2)
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how to add bases to the primer restriction sites - (reply: 2)
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Questions about Stratagene QickChange Multi-mutagenesis - about how many primers and template DNA size (reply: 5)
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primer design - (reply: 3)
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How to ressuspend primers for Sybr? - (reply: 3)
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simple primer calculation - (reply: 5)
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picking primers - (reply: 4)
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16s primers and PCR - (reply: 1)
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primer/probe mix for standard generation? - (reply: 2)
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Primers from RT-PCR database - RT-PCRdatabase primers giving primer dimers (reply: 1)
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restriction enzyme site with primers - (reply: 4)
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Help. Trying to sequence my plasmids, but the primers refuse to prime! - (reply: 1)
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weird problem with Primers! - problem with the gene sequence i used to design my primers (reply: 4)
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Forward n reversre primer Tm difference is 10 degree - (reply: 1)
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Primer mismatch bother me! - when enzyme sites added at 5' ends, mismatch increases. (reply: 1)
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primer design (downloadable) programs - not online tools (reply: 4)
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figure out the size of amplicon using known primer - (reply: 3)
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Primer concentration... - (reply: 5)
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microgram per microlitre primer to molarity conversion.. - calculation plss (reply: 1)
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Long PCR primers for epitope tagging - where to order? (reply: 3)
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***MSP Primer Troubleshooting Specificity*** - (reply: 6)
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No PCR product with verified primers on templates - Where is the bug???? (reply: 2)
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How good are my PCR primers? can I go with it?! - (reply: 2)
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QPCR primer Pairs - (reply: 1)
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primer design for unknown cDNA sequence - (reply: 1)
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touchdown qPCR - using degenerative primers (reply: 2)
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primer Tm for PCR mutagenesis - (reply: 1)
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Quikchange mutagenesis primer and PCR design - (reply: 3)
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Primer design - Issues with GAPDH primer blast search (reply: 2)
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primers dont match FASTA gene sequence... 'm confused - (reply: 11)
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sequence retrieval for primer design - (reply: 2)
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Primer conc - (reply: 6)
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Does anyone know how to design LUX primers - (reply: 2)
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Cox-2 primer for PCR - method how to search good cDNA in Pubmed (reply: 1)
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PCR primers for CRISPR - primer3 isn't helpful in this case (reply: 1)
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primer design for subclone - (reply: 2)
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Mutagenesis and primer purity? - (reply: 4)
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PCR primer design, real time versus old school - (reply: 2)
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I have a problem with the M13 tailed primer method - M13 tailed primers-SSR (plants) (reply: 1)
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cDNA synthesis using random hexamer vs. oligo dt primers - (reply: 2)
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PCR primers with overhangs - (reply: 2)
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Primer designing - (reply: 1)
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Designation of PCR primers in ChIP experiment - (reply: 3)
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What is your favorite tool for primer design ? - (reply: 2)
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Primer express? - Do you use it? (reply: 2)
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Bad primer design ? How can I know ? - How could I know if a primer would recognize a particular sequence ? (reply: 2)
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What is the proper upper limit of false priming in real time? - (reply: 3)
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Enzyme sites on Primer - (reply: 3)
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gene-specific Primer for qRT-PCR - (reply: 2)
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Designing RT-RCR Primers according to other species' homology - Using conserved regions as a guide when mRNA isn't known in your a (reply: 1)
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primer design for PCR cloning - (reply: 1)
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need help to evaluate primer sets for SYBR green qPCR - (reply: 2)
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PCR primers artifact - (reply: 2)
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qPCR primer efficiancy - (reply: 2)
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Primer Express by ABI - (reply: 2)
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primer design for site-directed mutagenesis - (reply: 3)
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what affects primer specificity? - (reply: 2)
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Assembly PCR - Design of PCR program for primers w/low Tm? (reply: 2)
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Primers PCR - storage (reply: 5)
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MSP: M and U primers products - (reply: 1)
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Human Epigenome Project primers? - (reply: 2)
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Creating short dsDNA from primers for cloning - (reply: 2)
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Degenerate Primers - Help with Degenerate Primers (reply: 1)
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PCR: Can you get amplification if only one primer anneals? - Same size pcr product using 2 different primer sets (reply: 8)
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ready to use primers for BSP - (reply: 4)
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Are there any special rules for primer design? - (reply: 5)
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what's the difference between sequencing primer and cloning one. - confused (reply: 5)
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sequencing issue - Internal primers (reply: 9)
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GAPDH primer sequences for mRNA level in HeLa cells - (reply: 2)
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RT PCR Primer dimer tissue dependent SYBR - (reply: 1)
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FGF20 PRIMER - (reply: 2)
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qPCR for microRNAs - minimal primer length? (reply: 6)
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primer design for Syber Green - (reply: 1)
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primer sets to do pyrosequencing - (reply: 3)
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primer walking design tool - (reply: 1)
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optimizing PCR for low Tm primers - (reply: 1)
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primer design for MSP and BSP - primers must be in promoter region? (reply: 1)
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primers TM - (reply: 8)
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Degenerate Primers and dI - (reply: 5)
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primer dissolved in wrong buffer (1M Tris HCl) - what to do now?? (reply: 7)
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designing a primer - (reply: 5)
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preference for region of primer design for qRT-PCR - (reply: 1)
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using primers programms - (reply: 9)
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qPCR housekeepers - primers or probes? - (reply: 1)
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PCR Trouble Shooting - new primer, wrong dilution of primers, wrong band, and many more (reply: 15)
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freaky primers - (reply: 5)
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long range PCR primer design - (reply: 1)
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How do you design primers of PCR-STR used in Forensic Medicine - Urgent!!!! (reply: 2)
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what is CDS primer used as a control for QPCR in CHIP? - (reply: 2)
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Primer tag info - (reply: 1)
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How to get rid of primer dimers - (reply: 2)
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problems with PCR primers - (reply: 15)
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one nucleotide in the primer is wrong - (reply: 2)
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Primer Dilution. ...water or buffer? - (reply: 15)
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real time PCR using two sets of primers with different melt curves - (reply: 4)
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Howto: Maximum possible yield from a PCR? - What to optimize: Mgcl/primers/Polymerase/enhancers? (reply: 4)
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Introduce restriction sites with PCR primers - (reply: 2)
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primer flaw I'm missing? - (reply: 7)
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MSP Primer design -rules to choose primers and reasons - (reply: 2)
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How long we can keep Diluted Primer? - (reply: 5)
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Primer calculation - (reply: 3)
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PCR primer resuspend method? - (reply: 9)
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Testing newly designed primers prior to real time - (reply: 2)
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About Degeneracy of a degenerate primer~~ - (reply: 2)
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information regarding PCR using very long primers - (reply: 4)
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MSP primer design - understanding the basics - (reply: 2)
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Bisulfite: PCR condition or primer problem? - (reply: 13)
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Primer concentration and standard curve slope in "Relative Standard Curve M - (reply: 1)
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storage of primers - (reply: 3)
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Curious - different primer synthesis? - (reply: 4)
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too many hits on Blast for primers / microsatellite - (reply: 4)
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Sequencing PCR products with biotin-labelled primer - (reply: 1)
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sequencing new "unknown" plasmid - any good SV40 primers out there? (reply: 1)
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how to get DNA sequences starting from the primer - (reply: 4)
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Primer orientation, forward and reverse - (reply: 4)
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primers for bisulfite-modified DNA: general questions - (reply: 6)
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Negative control primer for ChIP - (reply: 3)
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primers for promoter amplification - (reply: 1)
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RT-PCR primer designing - (reply: 6)
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PCR with overlapping primers - (reply: 1)
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Free program for primer designing? - Need to check promoter sequence for possible primer binding sites (reply: 3)
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Can any one suggest a company who can design a primer pair - dnaK PCR work (reply: 1)
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My primers don't work with Chip - (reply: 1)
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LNA Primers design - (reply: 1)
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qPCR using random primers - (reply: 4)
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designing primers for cloning - designing primers for cloning (reply: 1)
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Lambda DNA nanowires, Restriction sites and thiolated primers - (reply: 1)
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1. ABI Power SYBR® Green primer efficiency? - (reply: 1)
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Amplifying a 1700bp fragment using a mismatched primer pair - (reply: 2)
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Identification by 16S rDNA - using short primer (reply: 2)
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Degenerate Primers - (reply: 12)
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restriction enzymes primers - (reply: 5)
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Plasmid DNA Sequencing with Universal Primers - (reply: 3)
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Primer Efficiency (how to change the slope) - (reply: 5)
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Optimisation of dCAPS markers (degenerate primers) - problems with detection of heterozygotes (reply: 1)
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Ever heard not of Prime Dimers but Primer Multimers? - (reply: 1)
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Possible mutations in primers? - (reply: 4)
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How many of us request for PAGE purification of primers more than 30 mers in len - PAGE purified primers (reply: 5)
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Need to confirm PCR primers are bad - (reply: 5)
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cloning primers, TM, and specificity - (reply: 4)
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Help! Primer Dimers… - (reply: 3)
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Allele Specific PCR Primer Design - (reply: 1)
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Very low PCR primer concentrations? - (reply: 4)
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Question about BSP primer design? - What is the complementary strand for forward primer? (reply: 3)
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Primer sets for Bisulfite-modified and unmodified DNA - (reply: 6)
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Designing forward primer incorporating Nde1 cut site - Tips on cloning with Nde1? (reply: 2)
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Primer Design for Gene Inserted Counterclockwise into Plasmid - Please help...very basic question for a mol. biologist (reply: 1)
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PCR Primer again - (reply: 1)
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False priming in MSP - (reply: 4)
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Polymerase Choice Troubles - my primer melting temp is so low (~30 C) (reply: 4)
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Can primers go bad and give a smear in PCR? - (reply: 12)
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primer Tm - (reply: 10)
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QuickChange primers self-made - (reply: 4)
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oligos as synthetic "miRNA" for quantification? - would an oligo / primer work in AB RT kit? (reply: 2)
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PCR Conditions for primers with GC Clamp and without GC Clamp - (reply: 3)
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Non specific amplification - How to check if the primers give non specific amplification or not (reply: 4)
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digoxygenin-labelled primer - (reply: 3)
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How to import GeBank files into Primer Express 2.0 - (reply: 1)
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freezing of primers - (reply: 2)
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Primers with RE sites - (reply: 2)
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Primer dilution and calculation - (reply: 4)
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what can i do with this pcr? - amplification of cDNA , with primers tm 50 and 67 (reply: 7)
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Designing primer for Banana mild mosaic virus - (reply: 3)
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attaching t7 promoter sequence to template primer - (reply: 1)
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primer for real-time and reverse transcriptase PCR - (reply: 1)
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PRIMER 3 PROGRAMME - (reply: 8)
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Bisulfte PCR and sequencing PCR and Primer Notes - Tips and hints on PCR for bisulfite converted DNA and bisulfite primer (reply: 33)
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primers stopped working - (reply: 3)
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humanized antibody - how to design primers for CDRs - humanized antibody (reply: 1)
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Primers going bad - what's the best storage/usage conditions? (reply: 4)
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5' Primer modification - Tm-effect ?? (reply: 1)
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Designing PCR Primers - (reply: 3)
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sequencing - which primer should I trust? (reply: 4)
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running a gel with primers - (reply: 2)
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Help- degenerate primer design - (reply: 4)
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Will this primer work? - (reply: 8)
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Use of W primers to detect efficiency of bisulfite reactions? - (reply: 5)
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What are the most important parameters in PCR primer design? - Tm difference, GC clamp, GC%, primer-primer complementarity, self-complementarit (reply: 9)
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Is a Kazark sequence needed in a upper primer? - (reply: 17)
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Primers and enzymes not compatible - How can I fix this? (reply: 2)
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cytokines primers - (reply: 1)
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Single primer PCR - (reply: 3)
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Inverse PCR primers design - (reply: 4)
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Enzyme Site in reverse Primer - (reply: 3)
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general pcr/primer question - (reply: 1)
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for the PCR primer what is better the hairpin or the self annealing? - (reply: 9)
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primers in PCR - primer removed or not (reply: 5)
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Is this kind of primer OK? - (reply: 2)
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different primer efficiency using cDNA and plasmid/PCR product - (reply: 4)
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RNA in cDNA - Can I use the same PCR primers for the production of cDNA? (reply: 3)
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Primers for BS treated DNA - (reply: 5)
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Primer Design software - (reply: 8)
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concentration of primers - (reply: 5)
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Primer design - (reply: 4)
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forward & reverse primers - (reply: 3)
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Fusion protein questions - Botched deletion primer (reply: 2)
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Primers & probes of Applied Biosystems for real time - (reply: 2)
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Primer walking - (reply: 2)
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Methyl Primer Express from ABI - (reply: 2)
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Primer Optimiztion - can this be done in water (reply: 8)
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design primer - (reply: 5)
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increasing sensitivity and reducing primer dimmer - (reply: 11)
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can Primers for RT-PCR be used Real-Time PCR directly? - (reply: 2)
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Can DNA polimerase amplify primers itself? - need help please!! (reply: 7)
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primer design questions for confused - (reply: 2)
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Silly Question about RT-PCR/Primer design - (reply: 2)
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primer selection for RT prior to real time - an insiders hint!!! (reply: 1)
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Which primer concentration for RT with very low RNA amounts? - (reply: 1)
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primer concentration - (reply: 7)
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restriction site in primers urgent - (reply: 1)
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RT-PCR primer design: Over introns and exons? - (reply: 2)
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primer design problems - (reply: 4)
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SYBR VS TAqman- What is the difference other than PRimers? - TAqman works and SYBR doesnt (reply: 1)
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Which primers are better for colony PCR? - (reply: 4)
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pcr yield and primer concentration - (reply: 5)
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RT-PCR - not qPCR! urgent please - how to choose primers (reply: 3)
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Primer design with restriction sites - (reply: 3)
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Why there is use of only a single primer in case RAPD amplification - (reply: 1)
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differences between oligo and random primers - (reply: 2)
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RT-PCR primers - (reply: 3)
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Any primer design programs...? - (reply: 8)
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Problem SNP that genotypes differently using different primers? - (reply: 2)
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Cutting out of a T vector - Cutting sites in primers vs sites in vector (reply: 3)
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PCR problems, huge artifacts, no product - involves 16S primers (reply: 8)
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primer : shifting peak - (reply: 4)
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Primer design for BSP - (reply: 12)
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Designing primers and hybridization probes - (reply: 1)
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Random Primers vs Oligo(dT) primers? - (reply: 3)
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ChIP primer for promoter region of GAPDH - for taqman analysis (reply: 2)
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5' phosphorylated primers - (reply: 1)
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Methylation specific primer designing - (reply: 1)
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primer design with intentional mismatch - (reply: 3)
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Primer Extension Assay versus RACE-PCR - Protocol (reply: 1)
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please tell me more about primers with restriction site! - (reply: 4)
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RT-PCR primer design - (reply: 1)
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Primers work for regulary PCR but not real-time PCR - why? (reply: 8)
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how to check primers - (reply: 4)
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How to design primer for unknown DNA sequence? - (reply: 9)
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TM for primers with restriction secuence tail. - Suggestion (reply: 3)
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can primers age? - (reply: 1)
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How to determine PCR Annealing temperature for primers with different Tm - (reply: 7)
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poor random priming for Northern Blot probes - (reply: 1)
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PCR with 75 mer forward primer - (reply: 4)
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half life of primers - (reply: 2)
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about the primers in MSP - (reply: 5)
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What annealing temperature is better for my primers? - (reply: 7)
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primer dimer in pcr product - cloning for a gene. analyzing pcr product shows primer dimer (reply: 8)
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primer concentration (calculation problem) - (reply: 5)
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PCR, labelled vs unlabelled primers - (reply: 4)
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How to add restriction enzyme sites to the primer - (reply: 6)
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Using pentadecamer primers for reverse transcription - Better than hexamers, oligo d(T)? (reply: 7)
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primer:cross-dimer - How to remove the cross dimer? - (reply: 3)
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Bands appear poor/not at all and do not appear consistantly & primer bands a - (reply: 2)
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can I detect colony for the insert with the cloning primers? - or clamp sequence will interfere? (reply: 4)
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The weirdest primer ever - (reply: 7)
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oligo(dT) plus random primers in RT - (reply: 4)
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no RT controls - can I run on separate plate and for just one primer set? (reply: 1)
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primer calculation - concentration (reply: 2)
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What happens to PRIMERS at 72 C ? - (reply: 5)
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0,2uM is 100ng of a 18-mer primer - (reply: 8)
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Melting Curve giving flutating Ct values - Advice on primer design? (reply: 9)
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checking colonies by vector primers.... - (reply: 7)
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Direct BSP using tag-modified primers - (reply: 2)
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adding restriction sites to degenerate primers - (reply: 9)
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Mutagenesis using 4 sets of primer - (reply: 6)
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cloning primer and tag questions - first try (reply: 2)
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PCR analysis of clones - Can a gene specific primer pair amplify a fragment in the wrong direction? (reply: 1)
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Amplification of gene (CHH,MIH,GIH) from prawns. - Amplification of gene using primers designed on the basis of cDNA sequences (reply: 1)
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pro-PCR with only one primer... - to check directionality. (reply: 4)
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Two design programs give very different primer Tm values - RT-PCR (reply: 5)
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cDNA synthesis primer - (reply: 3)
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Primer with tag: Which is the real concentration? - (reply: 3)
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antigen primers - (reply: 1)
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How to design PCR primers in ChIP assay - (reply: 2)
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Annealing vs melting Ts for real time PCR primers - (reply: 1)
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relative quantification, primer pair problem - (reply: 1)
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Calculating Tm for bisulfite primers? - (reply: 3)
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Designing species specific primers for E.coli - please help? (reply: 3)
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Using primers meant for DIG probe as PCR diagnostic - (reply: 1)
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Using primers meant for DIG probe as PCR diagnostic - (reply: 3)
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Need for Nested PCR - Primers Designed with MethPrimer - (reply: 6)
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Topo Zero Blunt Vector by Promega Amplification of insert with M13 - Amplification of insert with M13 primers (reply: 1)
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Primer for 5' RACE - (reply: 2)
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Stick T7 on custom primer for sequencing? - (reply: 2)
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ABI have released MethylPrimer Express! - A new primer design program for DNA methylation....For Free!!! (reply: 45)
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MSP primer design help - (reply: 5)
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PCR using long primers and two overlapping templates - PCR using long primers and two overlapping templates (reply: 2)
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Primers for nested-PCR - (reply: 1)
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PCR primers with high Tm - (reply: 6)
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how to choose best annealing temp. for primers? - (reply: 6)
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Primer Dimer problem- Different perspective - COuld there be a treatment effect (reply: 4)
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PCR using a long (100nucleotides) and a short (20 nucleotides) primer. Problem: - (reply: 7)
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making primers for similar protein sequences - making primers for similar protein sequences (reply: 4)
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BSP and MSP Primers - (reply: 2)
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Bisulfite sequencing primer - (reply: 4)
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MSP Primer Sets (Herman Papers) - (reply: 1)
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random primers for qPCR - (reply: 3)
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QPCR, where to start? - optimizing primer and template (reply: 5)
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difficulty in Primer walking - (reply: 2)
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PCR primers Tm higher than extension T - (reply: 8)
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Primer for sequencing - need help!!!!!!!!!! (reply: 6)
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Primer Design - (reply: 2)
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Primer design invert pcr - (reply: 5)
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cloning two fragments with deletion in between - PCR primer design (reply: 3)
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Vector construction - Can I use long PCR primers (60bp) and get good PCR poduct??? (reply: 4)
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designing primers for Real Time - (reply: 1)
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Stem loop RT-PCR for miRNA quantification - how can I design the stem loop primer? (reply: 2)
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Methods for primer reconstitution - (reply: 23)
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BSP Primer placement over KNOWN unmethylated CpG's - Can this be done? (reply: 3)
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no result with pcr. is it the primer?/ - no amplification in pcr even after starting from the beginning (reply: 6)
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dimers of dna (not primers) following digest or pcr? - (reply: 11)
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can we add the terminaton codon in the 3' primer? - (reply: 1)
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Question about Realtime PCR primer concentration.... - (reply: 5)
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designing primer for methylation - (reply: 21)
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plasmid amplification - exons and primers (reply: 1)
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Primer dimers?Contaminations? - Unexpected bands! (reply: 5)
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reverse primer design with restriction site - (reply: 6)
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Reverse Transcription with oligo dT or hexamer primers? - What do you prefer and why? (reply: 3)
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question in 5' RACE and primer extesion - (reply: 8)
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Primer concentration ? - (reply: 2)
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PCR product dimer (not primer dimer!) - PCR (reply: 2)
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Q-PCR questions: Primer design and melting curve - (reply: 13)
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from which strand to derive BSP primers? - (reply: 5)
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PCR - free primer designation program (reply: 2)
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Primer Annealing for PCR - (reply: 4)
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cDNA library screening with 5' specific primer - (reply: 1)
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Site-Directed Mutagenesis- Doubt - Overlapping primers (reply: 3)
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Both the PCR primers in a vial. - Did anybody mix the diluted primers in single tube for use? (reply: 7)
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How to design primer for sequencing unknown DNA - (reply: 5)
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PCR - forward, reverse primers - is the reverse primer the "reverse complement" of the sense strand (reply: 2)
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need helps on probe design--human ip10 primers and probe. - (reply: 2)
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how to determin the Annealing Temp of ristriction sites modified primer? - help me ! (reply: 26)
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Sequencing Primers - (reply: 4)
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5' RACE - gene specific primer - (reply: 1)
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RT PCR on ALM patients - help for designing primers for detect PML RAR (reply: 2)
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About PCR primers' concentration - (reply: 2)
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Primer design with incomplete sequence - (reply: 2)
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Annealing temperatures for long primers? - PCR reaction (reply: 6)
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PCR primers from amino acids? - (reply: 1)
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Primer dimers - (reply: 1)
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primers design for mutagenesis of two nearby sites - mutations (reply: 2)
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design spesific primer of avr, hrp, toxin and falgella gene - (reply: 3)
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Intensive primer dimers - (reply: 2)
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PCR using different primers to generate same DNA sequence - explanation please :) (reply: 2)
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PCR is positive with one set of primers, negative with another. - (reply: 4)
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Do you synthsize your own oligo(dT) primer? - (reply: 2)
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Trouble with primers of BSP - (reply: 3)
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degenerate primer design - (reply: 4)
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potassium acetate in primer solution - I made a mess but need to recover (reply: 6)
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16S rRNA primers for E. coli - (reply: 2)
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primer design - how to design primers (reply: 2)
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Multiplex Primers - Primer primer interaction (reply: 1)
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Why primers for bisulfite converted DNA amplify unconverted DNA? - (reply: 3)
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RT-PCR primer design at 3'-UTR or ORF? - (reply: 2)
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Reverse transcription of poly-A tailed miRNAs using anchored Oligo dT primers? - (reply: 6)
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Primer design for environmental samples - Align sequences first? (reply: 3)
-
southern hybridizaion with 5' end labelled primer - (reply: 1)
-
Programs for designing Primers - (reply: 6)
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Disadvantages of using oligo-dT primers - (reply: 1)
-
How to eliminate primer dimer in MSP - (reply: 20)
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do primers and dNTPs alter digestion efficiency? - (reply: 4)
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Sequencing primer dilution - (reply: 2)
-
PCR with degenerate primer - (reply: 6)
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1.3kb PCR only shows primer dimer - (reply: 2)
-
RT-PCR primers for E. coli LacZ gene - (reply: 3)
-
water instead of TE buffer to dissolved primers - (reply: 6)
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primer reconstitution - (reply: 2)
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Question about PCR primer with degenerate base at 3' end - (reply: 2)
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Primer for 4 genes sharing short consensus sequence - (reply: 2)
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what is the best pair of sequencing primer for puc 18 - (reply: 1)
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Looking for online primer design for SNaPshot - (reply: 1)
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pCI sequencing primer - (reply: 1)
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PCR primers: hairpin and dimers? - what can I do to avoid this? (reply: 4)
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Reconstitute primers - (reply: 4)
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multiplex PCR for three sets of primers - (reply: 1)
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inner primer for BSP PCR? - (reply: 6)
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How many mismatches in point mutation primers? - (reply: 3)
-
Software available for Multiplex PCR primer designing - (reply: 3)
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single primer PCR - use a single primer to amplify regions between inverted repeats (reply: 1)
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PRIMER design - primer dimers (reply: 1)
-
Mouse MSP primers - (reply: 11)
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PCR primer: 2 restriction sites? - (reply: 4)
-
Primer Dimer problems in real-time PCR - (reply: 14)
-
primer orientation - (reply: 1)
-
Degenerate primers for nested PCR? - (reply: 1)
-
so lost! stupid quenstion about primers.... - designing the primers (reply: 2)
-
Reliable ChIP primers for Sp1? - (reply: 2)
-
house-keeping primers for Cos7 cells - (reply: 4)
-
increased primer dimer with template - (reply: 2)
-
designing the sequencing primer - (reply: 2)
-
free software to examine if two primers will make dimers - (reply: 3)
-
primer efficiency in real time PCR - need for a method explanasion (reply: 1)
-
Testing primers - (reply: 3)
-
design of PRC primers of full ORF - (reply: 3)
-
Two round MSP with the same primer - (reply: 1)
-
primer dimers - (reply: 4)
-
Designing a Primer within an exon - Consesus required - (reply: 2)
-
Nested primer PCR - (reply: 5)
-
difference between primer extension and RACE - (reply: 6)
-
designing QRT-PCR Primers - (reply: 11)
-
Primer - (reply: 1)
-
Optimizing primers for SYBR green qPCR? - Optimizing primers for SYBR green qPCR? (reply: 7)
-
rat 18s rRNA primer - designing primers (reply: 1)
-
Which MSP & BGS primer design tool do you "swear" by? - (reply: 9)
-
Designing primers for multiplex - BLASTing lots of primers (reply: 3)
-
Why random primers for RT-qPCR - (reply: 1)
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Primer design question - Use of primer 3 (reply: 3)
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Primer extension - yeast tRNA as a negative control (reply: 3)
-
RT-PCR Primer design - which region? - (reply: 2)
-
primer design for MSP - How many CpG in the primer? (reply: 2)
-
No PCR products in MSP with MethPrimer designed primers - (reply: 2)
-
problems with mix of MSP's primers - (reply: 2)
-
design primer for PCR.. - (reply: 3)
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From MSP primer to gene - how to recalculate original DNA sequence? (reply: 4)
-
Design primer - (reply: 2)
-
MSP primers not working - (reply: 1)
-
extra sequence in primers - (reply: 4)
-
Primer Programm for Sequencing - (reply: 3)
-
calculating Tm of primers - (reply: 8)
-
Primer extension problem - there are too many bands!! (reply: 1)
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How to design random primers - (reply: 2)
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design pcr primers - (reply: 3)
-
PCR primers for Mycoplasma detection? - Publication gives many, which to choose? (reply: 1)
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Does it help to add antisense primer first for bisulfite PCR? - (reply: 2)
-
primer design problem - (reply: 1)
-
Bisulfite treated DNA specific primer set critique? - (reply: 18)
-
60 bp PCR and primer dimers - (reply: 6)
-
No product/primer dimers after BSP - (reply: 1)
-
Primer Express Software - Probe Design (reply: 8)
-
Primer design:Is it ok to omit extra bases after RE site? - (reply: 4)
-
Problems with methylation analysis!!! - Primers crossreaction etc. (reply: 3)
-
primers for SoE - (reply: 1)
-
designing flanking primers around msp primers - (reply: 1)
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Primer for BSP - (reply: 1)
-
BSP cycles and primers - Is it OK to use the same primer set but with more (reply: 6)
-
primer hairpins and self complementation - online primer checker.. (reply: 2)
-
primer does not bind - help (reply: 2)
-
reconstitute primer - (reply: 4)
-
SOS call: ChIP assay in Huh7 cells - How many cells? And primer position? (reply: 3)
-
Online primer design - (reply: 7)
-
RT-PCR inconsistent primer efficiencies - RT-PCR (reply: 3)
-
Unmethylated primer control - (reply: 4)
-
is it effective to use a primer of SD rat to Wistar rat in PCR - help (reply: 2)
-
Custom Primers - (reply: 2)
-
Primers for RT reaction - (reply: 6)
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Primer Tm - How low can you go? - (reply: 3)
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How to prepare primer solution - (reply: 7)
-
Primers design: how to check for mispriming? - (reply: 2)
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primer storage - (reply: 7)
-
Pls check my bisulphite sequencing primers - (reply: 12)
-
Help: Check my MSP primers - is my MSP primer correct??? (reply: 1)
-
Bad Primer - (reply: 2)
-
reducing primer dimer - (reply: 1)
-
Calculating correct melting temperature for primers - (reply: 2)
-
primer melting temperature - (reply: 2)
-
Bisulfite Sequencing Primer Design - (reply: 3)
-
end of primer - (reply: 2)
-
degenerate primer desgining - degenerate primer desgining (reply: 5)
-
PCR primer dimer? - (reply: 7)
-
checking primer - checking primer (reply: 5)
-
PCR primer Tm and concentration question - (reply: 3)
-
How to avoid secondary structure in primer - (reply: 2)
-
cloning primer orientation - orientation and complement of primers in a cloning (reply: 3)
-
Random hexamer vs oligo dT vs gene specific primer for RT - which do you use most? (reply: 5)
-
primer design - checking off-target site - (reply: 5)
-
What is the distance between a forward and reverse primer - (reply: 1)
-
Which RT primer is better for qPCR - (reply: 2)
-
PCR; Perhaps someone can explain? - PCR; Primer (reply: 3)
-
Why different amounts of forward and reverese primers in RT-QPCR - (reply: 2)
-
Degenerate primers - (reply: 1)
-
primer optimization - (reply: 1)
-
primers for Q-PCR - (reply: 2)
-
multiplex PCR - template and primer concentration (reply: 2)
-
Primers and templates - Primer and template concentrations (reply: 2)
-
Real Time qPCR - qPCR SYBR greenI primer design (reply: 5)
-
Primers for species identification - Know any good? (reply: 6)
-
The perfect number of CpGs in MSP primer - (reply: 8)
-
Primer Express 2.0 - crashes (reply: 1)
-
Primers - (reply: 1)
-
How to measure the concentration of primers? - (reply: 2)
-
Probes or primers that span introns - (reply: 6)
-
Tm for my PCR product (not Tm for the primers!) - (reply: 3)
-
what primers specific for B.stearothermophilus?Help pls. - (reply: 5)
-
help regarding primers reconstitution - (reply: 4)
-
problem with my PCR - primer dimer. - (reply: 1)
-
PCR - Long PCR with short primers (reply: 1)
-
how long a primer should I use? - (reply: 1)
-
identifying exon and intron for primer design - (reply: 2)
-
what is the "tailed primer and taq primer"? - (reply: 2)
-
Designing methylation primers on long CpG island - (reply: 14)
-
Difficutly go separate product from primer dimers - Methylation specific PCR (reply: 1)
-
real time PCR primer for methylation - (reply: 2)
-
designing gene-specific primer for cDNA synthesis - (reply: 5)
-
Primer design for unknown gene - (reply: 4)
-
How to design primers for CpG, CpNpG, CpHpH methylation - Bisulfite Sequencing PCR (reply: 2)
-
Designing primers from amino acid sequence - cloning problem (reply: 3)
-
software to find restriction site and TM for primer - (reply: 7)
-
sense/antisense specific pcr - design of sense/antisense specific pcr primers (reply: 1)
-
product size for real-time PCR primer - (reply: 3)
-
GC content of PCR primers - (reply: 3)
-
real time PCR primers - (reply: 2)
-
mC not in CpG? Primer selection - (reply: 1)
-
cpgware primer design, does it work - (reply: 4)
-
Who will give me a software to design primer for PCR? - (reply: 3)
-
PCR Primer Design with restriction sites - (reply: 1)
-
primers with different Tms - PCR amplification using primers with different Tms (reply: 9)
-
Sequence input for bisulfite PCR primer design - (reply: 1)
-
Primer design for MSP queries - (reply: 3)
-
Database software to catalogue / archive primers - Database software to catalogue / archive primers (reply: 1)
-
Real Time PCR Annealing vs. Melting - Relation of anneal temp rxn to melt temp primer (reply: 2)
-
primer design for bisulphite treated DNA - (reply: 1)
-
primer dimers - what exactly are primer dimers (reply: 2)
-
primer design: end-point vs. RT - (reply: 1)
-
Primers for unmethylated DNA work too well! - (reply: 2)
-
software to check the primer dimer - (reply: 3)
-
Control for primer extension - (reply: 2)
-
BSP and MSP primer check - (reply: 2)
-
Using a big primer in PCR - (reply: 4)
-
How to get rid of PCR primer dimer - (reply: 65)
-
designed primer more powerful? - (reply: 1)
-
RT-PCR primer design - exon-exon primer design (reply: 5)
-
Primer design - PCR for cloning (reply: 6)
-
availabre primers for MSP - (reply: 1)
-
3' end of forward primer mismatch - (reply: 5)
-
RACE - specific primers in first strand synthesis (reply: 4)
-
qPCR Primer Design and Genomic DNA Amplification - (reply: 1)
-
Primer Designer Online - Pipleline use of designer (reply: 1)
-
If the primer is dimer,it's ok? - (reply: 5)
-
methylation primers - problems with primers (reply: 1)
-
Multiplex PCR primer designing - (reply: 4)
-
real-time PCR - How to design primers (reply: 2)
-
BiPolar Clamps on PCR primers for TGGE - (reply: 1)
-
PCR primers - PCR primers for RT-PCR (reply: 1)
-
multiplex primers - (reply: 6)
-
Primers: advice? - (reply: 1)
-
primer3 - select primer in 3' end (reply: 3)
-
bisulfite pcr primers - (reply: 1)
-
The PCR Suite - a set of new primer design tools - (reply: 1)
-
RT-PCR primer design guide - How to check gene structure and design the primer? (reply: 15)
-
Primer dimers - (reply: 1)
-
Double strand cDNA sysnthesis - Using random primers (reply: 1)
-
degenerate primer - optimizing degenerate primers (reply: 1)
-
Need suggestions for degenerate primers - (reply: 2)
-
Primer extension - (reply: 1)
-
Primer design - Primer design for RT-PCR (reply: 1)
-
I am afraid I got wrong Primer from company - (reply: 2)
-
Primer contamination - (reply: 2)
-
degenerated primer for nested RT PCR - (reply: 5)
-
questions about degenerate primer PCR - (reply: 2)
-
design a primer - how to determind a specific primer in a complete gonome? (reply: 1)
-
How to find/check the highly conservative sequence - RT-PCR primers (reply: 2)
-
Scorpion Primers? - Synthese of Scorpion Primer (reply: 1)
-
primer purification - search method for primer purifying (reply: 3)
-
primer design tools - primer designing (reply: 28)
-
Software for MSP (methylation-specific PCR) primer design - (reply: 3)
-
primer design - Deinococcus (reply: 1)