primer dimer annealing temperature - (Mar/10/2008 )
Hi
I am trying to determine roughly the annealing temp of two complimentary oligos (ie. to form a primer-dimer). I don't have qPCR available to me- are there any programs that will enable me to estimate this?
Thanks
-Andy78-
IDTdna.com has "OligoAnalyzer" that should do the job: http://www.idtdna.com/analyzer/Applications/OligoAnalyzer/
QUOTE (Andy78 @ Mar 10 2008, 08:57 AM)
Hi
I am trying to determine roughly the annealing temp of two complimentary oligos (ie. to form a primer-dimer). I don't have qPCR available to me- are there any programs that will enable me to estimate this?
Thanks
I am trying to determine roughly the annealing temp of two complimentary oligos (ie. to form a primer-dimer). I don't have qPCR available to me- are there any programs that will enable me to estimate this?
Thanks
-Captain_DNA-
QUOTE (Captain_DNA @ Mar 11 2008, 10:01 PM)
IDTdna.com has "OligoAnalyzer" that should do the job: http://www.idtdna.com/analyzer/Applications/OligoAnalyzer/
QUOTE (Andy78 @ Mar 10 2008, 08:57 AM)
Hi
I am trying to determine roughly the annealing temp of two complimentary oligos (ie. to form a primer-dimer). I don't have qPCR available to me- are there any programs that will enable me to estimate this?
Thanks
I am trying to determine roughly the annealing temp of two complimentary oligos (ie. to form a primer-dimer). I don't have qPCR available to me- are there any programs that will enable me to estimate this?
Thanks
Thanks!
-Andy78-