cytokines primers - (Dec/02/2006 )
Hi,
I need to quantify some citokynes mrna by real time pcr with sybr green (the genes are IL-1b, IL-6, IL-8, TNF and Interferons alpha and beta).
Since it's plenty of primers out there, the choice is not easy.
Someone has any (positive or negative) experience to tell me with any published primer for these targets?
Thanks for any suggestion.
-doglo-
QUOTE (doglo @ Dec 2 2006, 05:17 PM)
Hi,
I need to quantify some citokynes mrna by real time pcr with sybr green (the genes are IL-1b, IL-6, IL-8, TNF and Interferons alpha and beta).
Since it's plenty of primers out there, the choice is not easy.
Someone has any (positive or negative) experience to tell me with any published primer for these targets?
Thanks for any suggestion.
I need to quantify some citokynes mrna by real time pcr with sybr green (the genes are IL-1b, IL-6, IL-8, TNF and Interferons alpha and beta).
Since it's plenty of primers out there, the choice is not easy.
Someone has any (positive or negative) experience to tell me with any published primer for these targets?
Thanks for any suggestion.
There are set of primers from in vitrogen and Takara, you can search and find out.
-Mikevnjp-