Control for primer extension - (Nov/04/2004 )
Hi
Any one plz tell me what control should i take for primer extension of RNA.
-Praveen Bawankar-
For negative control, use no RNA or RNA from cells that don't express your gene.
For positive control, maybe include some gene for which transcription start site is known.
-paulina-
I have looked for a positive control, but I can´t find a gene which transcription start point is known.
-Mone13-