sequencing new "unknown" plasmid - any good SV40 primers out there? (Apr/23/2007 )
Hi,
I have a plasmid that i've got no (real) map, no sequence data, and no RE data on.
But i do know it has an SV40 site. Does anyone have any good primers that are used in the SV40 area?
Ta
Vetticus
-vetticus3-
QUOTE (vetticus3 @ Apr 24 2007, 12:44 PM)
Hi,
I have a plasmid that i've got no (real) map, no sequence data, and no RE data on.
But i do know it has an SV40 site. Does anyone have any good primers that are used in the SV40 area?
Ta
Vetticus
I have a plasmid that i've got no (real) map, no sequence data, and no RE data on.
But i do know it has an SV40 site. Does anyone have any good primers that are used in the SV40 area?
Ta
Vetticus
Hi,
Don't know about primer, but here is what I pulled out as SV40 early promoter, found in a number of plasmid:
gatctgcgatctgcatctcaattagtcagcaaccatagtcccgcccctaactccgcccatcccgcccctaactccgccca
gttccgcccattctccgccccatcgctgactaattttttttatttatgcagaggccgaggccgcctcggcctctgagcta
ttccagaagtagtgaggaggcttttttggaggcctaggcttttgcaaaa
Hope it could help
-Almasy-