Primer design program for Methylight - (Nov/11/2008 )

Hi,

how do you design your primers and probes for quantitative methylation detection (Methylight)?
Anyone that can recommend a nice program?

Thanks!

-molbio-

Design primers with MethylPrimer (google it) and make a probe that fits the primers and the converted sequence in primer3 or the IDT SciTools website.

QUOTE (molbio @ Nov 11 2008, 09:56 AM)

Hi,

how do you design your primers and probes for quantitative methylation detection (Methylight)?
Anyone that can recommend a nice program?

Thanks!

-stippled-

Hi

May I draw your attention to Beacon Designer?

Beacon Designer designs TaqMan® probes and methylation specific PCR primers for quantitative methylation detection (Methylight).

For details, please visit: http://www.premierbiosoft.com/molecular_be...methylight.html

Regards,
Fatema Sidhpurwala
Bioinformatics Scientist
PREMIER Biosoft International
3786 Corina Way
Palo Alto, CA 94303
www.premierbiosoft.com

QUOTE (molbio @ Nov 11 2008, 07:56 AM)

Hi,

how do you design your primers and probes for quantitative methylation detection (Methylight)?
Anyone that can recommend a nice program?

Thanks!

-fatema-

Anyone tried Beacon designer for Methylight applications?

-molbio-