ITP substitutes... what? - can I add ITP to my degenerated primers to lower the degeneracy? (Jan/13/2009 )
Hi guys, I need help...
I designed degenerated primers via VectorNTI.
The degeneracy is to high though...
The question is - can I add ITP to my pcr primers? To which nucleotides is inosine complementary?
thx in advance
kliper
-kpt.kliper-
you can use ditp (to add dI to your primer). dI mimics all so can be used as N.
it is a little pricey so you may want to use it strategically.
-mdfenko-
QUOTE (mdfenko @ Jan 13 2009, 03:55 PM)
you can use ditp (to add dI to your primer). dI mimics all so can be used as N.
it is a little pricey so you may want to use it strategically.
it is a little pricey so you may want to use it strategically.
thx!
-kpt.kliper-