ChIP primer for promoter region of GAPDH - for taqman analysis (Sep/05/2006 )
Hi everyone!
I perform chromatin immunoprecipitation experiments and want to analyse the efficiancy of my specific enrichment by taqman analysis.
Does anyone of you can tell me the sequence of primers/probe that work well in taqman analysis for the promoter region of human house-keeping genes?
I searched the internet but had no luck so far
Greetings & many thanks in advance!
-positive thinking-
These are primers from Activemotif chip kit for positive control
GAPDH For: 5´-TACTAGCGGTTTTACGGGCG-3´
GAPDH Rev: 5´-TCGAACAGGAGGAGCAGAGAGCGA-3´
Product size: 166 bp
-pcrman-
Thanks a lot!
I will use them to design a taqman probe!
Greetings
_______________________________________________________
QUOTE (pcrman @ Sep 5 2006, 10:07 AM)
These are primers from Activemotif chip kit for positive control
GAPDH For: 5´-TACTAGCGGTTTTACGGGCG-3´
GAPDH Rev: 5´-TCGAACAGGAGGAGCAGAGAGCGA-3´
Product size: 166 bp
GAPDH For: 5´-TACTAGCGGTTTTACGGGCG-3´
GAPDH Rev: 5´-TCGAACAGGAGGAGCAGAGAGCGA-3´
Product size: 166 bp
-positive thinking-