RT-PCR - not qPCR! urgent please - how to choose primers (Oct/06/2006 )
My question:
When I pick the primers for the RT-PCR, should they match the unspliced mRNA or the CDS (the spliced variant that will be translated and starts with the ATG)???
-Jou-
It depends upon if the cDNA was synthetized from mature mRNAs or total mRNAs. If you used poly-A primers for the cDNA, the cds match should work. Good luck!
-aleruiz-
primerbank may be useful too.
-fred_33-
thank you for the advice!
-Jou-