checking primer - checking primer (Jun/15/2005 )

Hey all
Is it possible to check if my primers are correct without performing PCR itself?

Thanks

---

Why do you mean by "correct" and what exactly do you want to check? There are so many things to check for a pair of primers such as whether they align to the right target, whether there is inter-primer and self complementation, Tm value, etc...

---

I guess you can find out several software to access the paramters of your primers, however, I think, we can not say anything regarding the successful of the PCR without a run.

---

You can check your PCR probes "in silico" here:

http://scitools.idtdna.com/scitools/Applic.../OligoAnalyzer/

---

I think U can test them for the TARGET sequence at UCSCs InsilicoPCR..whcih will tell you what sequence U will get with this primers

chenna

---

I have designed primers for PCR and I wanted to check that the primers would give a successful reaction. Is there a way of doing this without doing PCR in the lab?

---