Programs for designing Primers - (Jan/15/2006 )

Hi,

what kind of (free) programs do you use to design primers and what experience do you have with it?

Recently I started to look a little bit on the internet for primer designing programs. I found "FastPCR". It is a good program but maybe there are better programs that I don't know yet.
Maybe you can help me with this.

Thanks

Try if you can get Primer Express by ABI Prism, or check this link PRIMER3

I've only got good experiences with primer3. You can also use amplifiX to make "in silico" PCR to test your primers.

Some of the companies selling oligo's will have primer design programs on their website (Sigma, IDT).

LeserattePD

Hi,

what kind of (free) programs do you use to design primers and what experience do you have with it?

Recently I started to look a little bit on the internet for primer designing programs. I found "FastPCR". It is a good program but maybe there are better programs that I don't know yet.
Maybe you can help me with this.

Thanks

Hi,

what kind of (free) programs do you use to design primers and what experience do you have with it?

Recently I started to look a little bit on the internet for primer designing programs. I found "FastPCR". It is a good program but maybe there are better programs that I don't know yet.
Maybe you can help me with this.

Thanks

i use oilgonucleotide properties calculator for primer designing and it is quite user friendly.

I have used Primer Express and also Primer3; Invitrogen has a nice one on their website as well