Temperature sensitivity of Plasmids - (Jun/15/2014 )

Hi, i'm working concurrently in  two projects involving plasmids that are temperature sensitive, one of them is said to replicate at 28ºC and the other at 30º C , However, we have only two incubators at the lab and i couldn't take both of them and is making things difficult for me. Could I grow the bacteria of one plasmid in the other's temperature? is 2º a catastrophic difference for the plasmids? thanks a lot it will save me a lot of time or at least will help me explain other people why i would be using the two incubators.

Raygoza on Mon Jun 16 03:28:50 2014 said:

Hi, i'm working concurrently in  two projects involving plasmids that are temperature sensitive, one of them is said to replicate at 28ºC and the other at 30º C , However, we have only two incubators at the lab and i couldn't take both of them and is making things difficult for me. Could I grow the bacteria of one plasmid in the other's temperature? is 2º a catastrophic difference for the plasmids? thanks a lot it will save me a lot of time or at least will help me explain other people why i would be using the two incubators.

 

 

 

 

To be honest: I wonder how you are going to checkt that 2°C difference!

Many incubators shift between (lets say) 35 and 38°C when they are set at 37°C...

And thats not even talking about people opening them to take stuff out and so on...

28 or 30°C will be fine for both plasmids...

(I am guessing the plasmid has a heat sensitive origin of replication? In most cases: this is more something that has a certain boundary, like for example: "will not replicate anymore abote 40°C" or something like that, but being sensitive to a specific certain temperature for replication itself is a bit weird.. it seems to be more an ideal temperature that you mean) 

You are sure its the plasmid and not the bacterium that is heat sensitive?