confusion about 5'UTR - confusion about 5'UTR (Apr/07/2009 )

hi
My confusion is about subgenomic replicon of hcv. In almost all papers to make subgenomic replicon ,they add two cistron , one of hcv 5’utr and 2nd after neomycin gene EMCV.
I cnt understand still now after reading many literature why they do this,why there is need of TWO cistron. (as I know 5’utr contain IRES, helps ribosome to translate.)


And 2ndly if some one dnt add 5’utr and EMCV. Before any of the structural or nonstructural gene. Does it be called subgenomic. For example I am want to make E1,E2 stable cell line,, but these sequences doesn’t contain 5’utr and EMCV.E1E2 will be transcribe under promoter of expression vector.can we call it stable subgenomic replicon.

Plz solve my confussion. I’ll be GREAT THANKFULL.if any one.

Hi Chicken,

Easter is coming.
Some people are on holiday or are busy finishing the experiments before the holiday.

After Easter Holiday, there will be more people to answer the posts.