how i can get insert back? - (Aug/14/2010 )

Hi everyone,

can any one tell me how i can get my gene of interest back after having insertion in T/A cloning vector (like pTZ57R/T) as I have no place any restriction sites in gene while doing amplification?

awaiting
af malik

Do a pcr using the primers that you had cloned your insert, and the cloned vector as your template. is this what you want?