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Is it possible to blunt only either the 5' or 3' end? - (Apr/24/2011 )

Is it possible to blunt only either the 5' or 3' end of a DNA fragment or do all blunting enzymes blunt indescriminately?

-seanspotatobusiness-

seanspotatobusiness on Mon Apr 25 00:45:42 2011 said:


Is it possible to blunt only either the 5' or 3' end of a DNA fragment or do all blunting enzymes blunt indescriminately?


I contacted NEB and they said exoT with cut 3' overhangs and RecJf will cut 5' overhangs.

In any case, neither are necessary if I cut with one enzyme and blunt that end before denaturing and cutting with the enzyme whose sticky ends I want to keep!

-seanspotatobusiness-