competentce cell, cloning - (Aug/15/2011 )

how do i make more competence cell from my E.coli BL21(DE3) competent cell? by growong up and glycerol stock? for plasmid transformation and protein expression proposal...

As usual, grow it, and use Calcium chloride method or others as shown:

http://openwetware.org/wiki/TOP10_chemically_competent_cells
http://openwetware.org/wiki/Preparing_chemically_competent_cells_%28Inoue%29

http://openwetware.org/wiki/Transforming_chemically_competent_cells
http://openwetware.org/wiki/Transforming_chemically_competent_cells_%28Inoue%29

Hope this helps.

Thanks Adrian...