Competent cells question: JM101 vs DH5alpha - (Apr/03/2012 )
I've been having difficulty tracking down E. coli JM101 in my lab's freezer inventory. I'm trying to replicate a published experiment that utilizes JM101 as a host for intermediate plasmids (pBluescript + cloned genes + resistance marker), and I'm wondering if I can just use DH5alpha as host to replicate these intermediate plasmids. Are there any issues with doing this?
-Steve Hwang-
I'm guessing it would be easier with DH5a. JM101 has an intact E. coli restriction system, which will reduce transformation efficiency for some constructs. It also is recA+, which may make some constructs less stable. Is this a paper from 1980?
-phage434-
It's a paper from 2003. But thanks for the info!
-Steve Hwang-