How large need homology arms to be? - (Feb/07/2017 )
I would like to insert a 2808 sequence bihind a gene by CRISPR/Cas9 homology repair. Are 800 bp-homology arms large enough or should they be larger?
Thank you
LP
-Luca-
800 bp is usually enough for these sorts of things. In theory you should be able to get away with much shorter - as little as 100 bp possibly.
-bob1-