|
Purification Related Discussions
-
Purifying Phosphorylated protein - (reply: 2)
-
230nm peak with NanoDrop - after gel purification (reply: 2)
-
Purification of GST FUSION PROTEIN - (reply: 1)
-
Single colony purification by streaking - (reply: 4)
-
protein purification from media supernatant? - protein purification of ECM proteins (reply: 2)
-
GST protein purification - (reply: 1)
-
how to purify amplicon off DMSO - (reply: 3)
-
storage of purified proteins? - (reply: 1)
-
expanded band of the purified protein on silverstain gel (even in low expression - (reply: 6)
-
His-tag purified protein is yellow! - (reply: 7)
-
Phenol/chloroform vs Column purification for ChIP DNA - (reply: 8)
-
general protein purification from mouse tissue - (reply: 4)
-
Protein purification by two stage chromatography - MDH by anion exhange & gel filtration (reply: 1)
-
Adenoviral purification by CsCl - Only cell pellet or supernatant too??? (reply: 5)
-
protein purification from Pichia - Is there any good way to kill the protease activity? (reply: 2)
-
Purification of gst and His tagged protein - after purification there are still many contaminated bands (reply: 1)
-
Protein purification? - urgent (reply: 3)
-
superscript indirect cDNA labeling system - superscriptIII, cDNA purification, microarray, free amines (reply: 3)
-
His-TAG purification - (reply: 1)
-
How to reuse DNA purification columns? - (reply: 3)
-
How to purify unknown protein? - (reply: 2)
-
native purification of His-tagged proteins from E. coli? - (reply: 1)
-
GST tagged protein purification - (reply: 8)
-
protein purification - (reply: 4)
-
how to purify the primary preadipocyte culture? - many fibroblast contamination (reply: 4)
-
Purification of RF DNA from phage prep - Need to get rid of ssDNA contamination (reply: 2)
-
Caspase 3 assay - on purified proteins (reply: 4)
-
Troubleshooting protein purification cation IEX - Cation IEX (reply: 1)
-
Protein purification - (reply: 1)
-
qiagen dna purification columns - are they all the same? (reply: 2)
-
Protein purification issues - (reply: 4)
-
DNA purification after sonication - (reply: 12)
-
Taq polymerase - Taq purification (reply: 3)
-
QIAquick PCR purification kit for in vitro transcription? - (reply: 6)
-
Yield after column purification - (reply: 3)
-
purifying small DNA fragmets - (reply: 2)
-
purification of non his-tagged proteins - (reply: 4)
-
Protein purification - (reply: 5)
-
CHIP / Is it normal to see pellet after the DNA purification? - (reply: 3)
-
Purified cDNA Clonning - (reply: 3)
-
DNA Dialysis - DNA Purification (reply: 1)
-
polysaccharide purification - (reply: 13)
-
GST or His tag for protein purification? - Tag choise + native vs denaturing conditions (reply: 2)
-
Purification granulocyte factor - (reply: 2)
-
RNA purification, am I going too far?! - (reply: 3)
-
Which quantity of purified protein in WB? - (reply: 2)
-
Which quantity of purified protein in WB? - (reply: 1)
-
mRNA purification - (reply: 2)
-
Ni-TED vs Ni-NTA - His-Tag purification (reply: 4)
-
Western Blot with HPLC purified samples - non specific binding??? need help desperately (reply: 1)
-
How to purify biotin labled DNA from mix - (reply: 2)
-
Problem purifying GST-fusion expressed in Baculovirus - (reply: 1)
-
Need to sequence gel purified vectors exposed to UV? - (reply: 2)
-
protein purification from yeast - (reply: 2)
-
High Yielding Plasmid Purification (suggestions) - (reply: 1)
-
Purification Optimisation - (reply: 4)
-
protein expresses using pGEX4T-3 but not being purified? - trapped in GST (reply: 8)
-
Dna purification from gel - (reply: 20)
-
Cheap and best method for full length m-RNA purification - (reply: 1)
-
Protocol for IgG Purification from Ascites? - (reply: 1)
-
purification of single stranded cDNA? - (reply: 3)
-
sequential PCR and purification before TA cloning - (reply: 1)
-
purification failed - how to restart? - Protein diluted in many fractions (reply: 7)
-
Problems with purifying a secret His-protein expressed in plant - (reply: 3)
-
GST tag protein purification - (reply: 2)
-
Problem with His Tag purification - using Promega HisLink spin purification kit (reply: 1)
-
Purification of buffy coat - Can look through and provide advice? (reply: 3)
-
do i need to desalt my protein after purification - (reply: 1)
-
how to improve the gst protein purification? - (reply: 1)
-
Gel purification of large plasmids for PCR or sequencing, HELP! - (reply: 3)
-
purification、ligation and transformation problems - (reply: 11)
-
Purifying protein with no his tag - (reply: 5)
-
linearized vector purification from TAE agarose gel - (reply: 5)
-
Purified PCR product - (reply: 2)
-
MoBio Plasmid Purification and Gel Extraction Kits - Problems with MoBio Kits (reply: 3)
-
how to refold insoluble protein after purification - (reply: 10)
-
Will TOPO TA cloning work on "old" purified PCR product? - (reply: 4)
-
Collagen IV purification - (reply: 2)
-
Alpha-Amylase expression and purification - (reply: 3)
-
DNA gel purification without a spin column - (reply: 4)
-
Purification Rookie - advice appreciated - (reply: 2)
-
Peptide purification - (reply: 1)
-
Buffer Exchange & Protein Purification - (reply: 1)
-
storage buffer for purified protein - (reply: 3)
-
Purifying small fragments - (reply: 9)
-
Gel or column purify - (reply: 4)
-
300 bp more after PCR purification - please help - (reply: 3)
-
Big problem in GST Protein purification - (reply: 9)
-
preferred method for purifying antibodies - (reply: 2)
-
protein purification - (reply: 5)
-
Protein G purified IgG>>> extra band at approx 200 kd - (reply: 5)
-
IgG purification: Multimerization... - (reply: 4)
-
96 WELL PCR Purification Suggestions - (reply: 4)
-
Solutions for manual RNA purification - (reply: 2)
-
Affinity Purified Antibody - (reply: 1)
-
His tagged protein purification - (reply: 2)
-
purification of HRP antibody conjugate - (reply: 10)
-
storage of ammonium sulphate purified antibodies - (reply: 1)
-
Please help me! I am really tired of this stupid protein purification! - (reply: 8)
-
How to purify 700bp pcr product? - (reply: 5)
-
Superdex or Sephacryl? - protein purification by size exclusion chromatography (reply: 7)
-
Gel purification: SpinX vs commercial kits - Gel purification (reply: 3)
-
protein complex purification taptag - (reply: 2)
-
no aminoallyl-dye conjugation after purification - (reply: 3)
-
what's the difference between the purification of GST-fused protein and His- - (reply: 4)
-
the problem in gel purification - (reply: 4)
-
QIAEX II gel purification kit - (reply: 11)
-
PCR purification /DMSO / vanishing DNA - (reply: 2)
-
HELP estimation of protein in ammonium sulphate purified igG - (reply: 6)
-
mRNA purification, stratagene - principles of mRNA oligo(dT) magnetic particles (reply: 3)
-
Problem in purifying fusion protein with His Tag - Fusion protein found in Flow Through (reply: 6)
-
simultaneos DNA purification - (reply: 5)
-
DNA Purification - (reply: 2)
-
Remove albumin from purified protein sample? Help please - (reply: 5)
-
Size exclusion chromatography for 23 and 20.5KDa? - protein purification (reply: 3)
-
Couple peptide to beads? for antibody purification - (reply: 1)
-
can i reuse the dna purification coulmn again - (reply: 1)
-
Methods for large-scale protein purification - (reply: 5)
-
Endotoxin Removal - Protein purification (reply: 2)
-
affinity purification of antibodies - what is the best buffer to elute (reply: 2)
-
purification after digeation - (reply: 3)
-
pcr product purification - (reply: 7)
-
Small Scale Bacterial Expression - protein purification (reply: 4)
-
DNA yields with the Qiagen PCR gel purification kit - (reply: 8)
-
Purification of digested PCR product (a smear fr 200bp to 3kb) - (reply: 1)
-
how to purify ChIP DNA by using kit - (reply: 4)
-
Protocol for AAV purification? - (reply: 6)
-
Weird two bands of my purified vector DNA apeared on my gel ? - (reply: 5)
-
Question about purification of GST-taqqed protein - (reply: 2)
-
Purification of HisTag Recombinant Protein - Advices needed (reply: 2)
-
removing rbc cells for cell purification - (reply: 5)
-
Affinity purification of antibody - (reply: 2)
-
protein purification problem - (reply: 3)
-
Problems after gel purification of plasmids - Restrictases and ligase do not work on gel purified DNA (reply: 2)
-
Buffers for MONO S purification - (reply: 1)
-
Protein purification with HPLC - (reply: 6)
-
Protein in each elution step during His-Tag purification - (reply: 4)
-
GST-fusion protein purification from bacteria - Several questions on protein purification (reply: 3)
-
tag for purification... - wich one do you prefer ? (reply: 10)
-
Gel purification of plasmid after each of these steps? - (reply: 1)
-
FLAG tag purification. - (reply: 4)
-
Sodium Citrate in DNA purification - (reply: 4)
-
Ligation of purified PCR product to Topo plasmid (with T7 promoter) and Transfor - (reply: 2)
-
DNA purification technique - After a PCR (reply: 8)
-
Protein purification - (reply: 2)
-
RNA purification from pancreas - troubles with the pancreas (reply: 1)
-
HELP! contaminants do not separate from my protein during purification - (reply: 5)
-
double tag purification. - (reply: 1)
-
plasmid purification - (reply: 3)
-
Low concentration after gel purification - PCR product purification (reply: 6)
-
Purification of 13 kb plasmid from E. coli - (reply: 4)
-
gst purification, got extra band - (reply: 4)
-
PCR and Digest cleanup before ligation. - A shortcut by avoiding gel purification. (reply: 15)
-
Gel purification of DNA bands-any alternates? - (reply: 4)
-
Protein purification - (reply: 2)
-
unsuccessful gel purification - (reply: 8)
-
gel purification for PCR product - (reply: 5)
-
How can I purify the wanted protein - (reply: 10)
-
Quantifying gel purification products - (reply: 12)
-
HA-protein purification - (reply: 1)
-
GST fused protein purification - (reply: 4)
-
Question for purchasing purified protein - (reply: 3)
-
About the ammonium sulphate precipitation? - prootein purification (reply: 10)
-
Purifying His-Tagged Proteins from Insects cells on IMAC resin? - (reply: 1)
-
promlems in protein purification in native form - (reply: 2)
-
purification of proteins from sample - (reply: 12)
-
protein purification - (reply: 5)
-
dialysis tubes for chromatin purification - (reply: 5)
-
mutant protein expression & purification problem in E.coli - Protein with five residues mutated to Alanine's (reply: 6)
-
His tag protein binding to resin ... - can i purify off the resin? (reply: 5)
-
GST-protein purification: degradation product - (reply: 19)
-
How long can U store DNA Sample - DNA Purification (reply: 3)
-
How to improve 260/280 reading for DNA? - 260/280 for purified digested plasmid is very high. (reply: 2)
-
purification prblm - big blunder (reply: 4)
-
GenScript- Free PCR Reagents and DNA Purification Kits! - (reply: 1)
-
GST and His tagged proteins - Does the GST give problems in His tag purification? (reply: 5)
-
Ask for recommendation of GST fusion protein purification kit - (reply: 1)
-
serum purification? - (reply: 6)
-
How to purify protein secreted in medium - (reply: 2)
-
Protein purification - (reply: 3)
-
Loss of phosphorylation after gel purification? - (reply: 3)
-
How many of us request for PAGE purification of primers more than 30 mers in len - PAGE purified primers (reply: 5)
-
Protein purification - (reply: 3)
-
CsCl density for BAC purification - (reply: 1)
-
purify radioactive labeled oligonucleotides - problems with the yield (reply: 1)
-
DNA ligation - problem with purification (reply: 4)
-
dna purification - (reply: 1)
-
Purification of GST-fusion - GST-fusion protein: How to express and purify (reply: 7)
-
UV light, dangerous to purify PCR fragments? - (reply: 2)
-
how to purify plasmid and genomic dna - (reply: 3)
-
Antibody purification protocol --any suggestions? - (reply: 12)
-
Specific and Total Activity for Protein Purification - (reply: 2)
-
Purified protein has no activity - (reply: 8)
-
Protein purification after cleavage (thioredoxin) - (reply: 3)
-
protein complex purification, western shows tag protein was pulled out, but silv - (reply: 10)
-
Epithelium cell purification - (reply: 2)
-
Which water is better for chemical preparation ? - water grade for protein purification (reply: 2)
-
large protein degradation - during baculovirus purification (reply: 2)
-
Endothelial cells purification - (reply: 2)
-
on-collumn DNASe in RNA purification - (reply: 3)
-
How to purify a sticky protein - (reply: 1)
-
will purification processes lead to loss of RNA? - (reply: 1)
-
problems with purifying a 100 bp DNA fragment - (reply: 12)
-
ha-tag purification , lots of bands - (reply: 4)
-
useful tips for low melting temperature gel purification. - please share your experiences. (reply: 2)
-
Purification and cloing of large fragments - cloning (reply: 3)
-
ethanol precipitation before gel purification... - (reply: 5)
-
Purification after oligos phosphorylation? - (reply: 1)
-
purification of Flavivirus - (reply: 2)
-
visualizing purified protein - (reply: 9)
-
Polyclonal antibody purification? - how? (reply: 9)
-
Protein purification for rabbit injection - SDS-PAGE gel, excise band, inject into rabbit?? (reply: 5)
-
A protocol needed to purify cancer cells from tumor tissue - need help! (reply: 4)
-
antibody purification - how to purify from serum free medium (reply: 1)
-
Purification protein from inclusion bodies - (reply: 4)
-
in vitro translation - how is the protein purified? (reply: 12)
-
Starting a purification.. - UF? IEC? HIC? SEC? What wud i start with? (reply: 4)
-
Purification of protein from plasma - How to separate a 30kDa protein from serum albumin (reply: 2)
-
Adenovirus Purification: CsCl solution sould be fresh? - Double CsCl Gradient (reply: 1)
-
plasmid purification from mammalian cells? - (reply: 1)
-
plasmid purification from mammalian cells? - (reply: 4)
-
GST purification multiple band problem - GST purification problem, multiple band (reply: 3)
-
Purified Protein Aggregation/Multimerization Problem - (reply: 3)
-
Protein purification before mass-spec - need sample purification methods (reply: 4)
-
Techniques to isolate large size DNA - large size DNA purification from mixture with small size DNA (reply: 4)
-
what these components do in the buffer used in protein purification - (reply: 5)
-
problem in protein purification phenol by phenol extraction - (reply: 1)
-
his-tag thrombin cleavage in-column? - as a purification strategy (reply: 1)
-
purification of catalase from liver? - (reply: 5)
-
Gel purification of PCR products - (reply: 2)
-
How to purify the ChIP DNA - (reply: 4)
-
Purification kits - Free samples (reply: 8)
-
GFP labelled protein purification - (reply: 1)
-
Purify lambda dsDNA bound to dye from unbound dye - (reply: 1)
-
can we purify the protein from westernblot membrane? - (reply: 5)
-
phenol/CHCl3 vs purification from gel - (reply: 1)
-
Affinity purification and binding pH - (reply: 4)
-
dna purification..help - plz..help (reply: 2)
-
Protocol for Re-ethanol precipitation of already purified DNA - (reply: 3)
-
digest, blunt end, religate, digest.... - so many steps, maybe can skip purification... (reply: 2)
-
ssDNA purification - (reply: 2)
-
Purified protein (his-tagged) multiple bands on gel - all picked up by antibody - what could they be? (reply: 2)
-
PMSF and tissue culture - Can you apply PMSF purified proteins to cultured cells? (reply: 2)
-
[WTA] Phyto-pathogenic Protocol. - how to carry out isolation, extraction and purification of phytopathogen from pl (reply: 1)
-
~25kDa UPP (Unidentified Purified Protein) after HIS-purification - (reply: 1)
-
dna purification - (reply: 6)
-
thrombin cleavage and triton? - protein purification (reply: 2)
-
How do you purify membrane protein? - help (reply: 2)
-
Protein purification with sephadex? - (reply: 2)
-
gel purification of PCR product - (reply: 1)
-
PMSF in AChE purification :unsure: - (reply: 1)
-
trouble getting cysteine labeled with biotin-maleimide - cysteine scanning purified protein (reply: 1)
-
%recovery of 26mer-oligo-T purification by ethanol precipitation is very low - other oligonucleotides were about 80-95% (reply: 3)
-
Lost: protein. Last seen prior to purification. - not present in flow through, washes or elutions! (reply: 2)
-
GST-tagged protein purification. - inclusion body formation of my GST taggged protein. (reply: 3)
-
Question about the strategy of protein purification - (reply: 4)
-
Help with Ni-NTA purification of EPSP Synthase protein - EPSP not binding to Nickel beads (reply: 5)
-
RNA extraction and purification - (reply: 6)
-
His tag purification - (reply: 1)
-
How to get rid of contaminated nucleases from purified proteins - (reply: 2)
-
Asking help for purifying plasmids - (reply: 9)
-
Asking help for purifying plasmids - (reply: 3)
-
protein purification -- 2 or more columns - (reply: 9)
-
Protein Purification - (reply: 1)
-
Gel purified samples not sinking in gel well's - Not getting my samples to fall to bottom of well (reply: 8)
-
Should I purify digested vector before ligation? - (reply: 7)
-
contaminating bands in Ni affinity purification - (reply: 5)
-
Do I have to purify vector after RE digestion for cloning? - (reply: 17)
-
mouse IgE purification - (reply: 1)
-
Protein Purification - Problem in protein purification (reply: 6)
-
biotinylated proteins purification from free biotin - dialysis vs gel filtration or what else (reply: 10)
-
NI-NTA colunm purification - no bands after purification (reply: 2)
-
in phenol/chloroform purification of nucleic acid, do you need to control pH of - (reply: 5)
-
buffer for His-tag recombinant protein purification - (reply: 2)
-
GST fusion protein purification - (reply: 9)
-
Problems in DNA sequencing after gel purification - (reply: 9)
-
how to purify fusion proteins on a large scale? - (reply: 2)
-
nested PCR: purification? - (reply: 1)
-
how can i preserve the purified mAb for a long time? - (reply: 7)
-
purification failed....please tell me what to do.... - (reply: 6)
-
tRNA expression and purification - (reply: 1)
-
DNA purification for transfection - someone have a protocol??? (reply: 1)
-
His Tag protein purification - (reply: 3)
-
Protein purification problem - protein purification problem (reply: 1)
-
Purification through the Ni-NTa column, multiple band, help - (reply: 8)
-
Affinity purification question.....do not want to couple with NH2 groups - (reply: 2)
-
identification and purification of hormone - protocols + knowledges needed about hormone (reply: 1)
-
Cell line to be used for protein expression an purification - (reply: 8)
-
Reasons for enzyme purification? - (reply: 3)
-
His tag protein purification problem - Binding of undesired protein (reply: 6)
-
Tips for transformation - which volume of ligation product? purification before transformation? Quantity o (reply: 4)
-
Anion exchanger chromatography for virus purification - (reply: 1)
-
How to extract and purify plant proteins? - (reply: 1)
-
Plant protein extraction and purification - how to extract plant proteins and purify them? (reply: 1)
-
aggregation of purified recombinant protein on dialysis-what do to? - (reply: 1)
-
Genomic DNA purification protocol - (reply: 6)
-
Is it possible to harvest E.coli cells for purification of DNA from LB agar and - (reply: 5)
-
shRNA insert purification - (reply: 4)
-
problems whit protein solubility after purification - (reply: 5)
-
siRNA purification - (reply: 2)
-
Removal of etoh from purified samples - my gel purified samples contaminated with ethanol. how to get rid?? (reply: 6)
-
a problem of genomic DNA purification - (reply: 3)
-
quick question about insoluble his-tag purification... - (reply: 2)
-
protein complex purification, what would affect? - (reply: 3)
-
how to isolate and purify nucleoprotein? - (reply: 1)
-
two step purification (SPA) concern change to one step? - (reply: 5)
-
Help on PCR purification - (reply: 2)
-
Gst tag purification problem - (reply: 5)
-
Purified antibodies in Western. - How can I find out how much to use? (reply: 12)
-
Protein purification wash problem - (reply: 1)
-
protein storage following purification - (reply: 1)
-
adenovirus purification - (reply: 1)
-
protein purification - (reply: 5)
-
problem with bacteriocin protein purification - (reply: 2)
-
enzyme purification - (reply: 3)
-
at which step can I store the protein? - purification from inclusion body... (reply: 3)
-
GST protein purification from inclusion bodies... - will GST part still be intact? (reply: 2)
-
purification problem. - most of my protein is washed away.... (reply: 10)
-
Should I use LMT Agarose to purify genomic DNA? Help please! - (reply: 1)
-
Help me! No colonies! Problem with gel purification? - (reply: 5)
-
What is the role of these gradient in SPA tag purification? - (reply: 1)
-
Purification of His-tagget protein from inclusion bodies - (reply: 12)
-
peptide purification method - (reply: 3)
-
suggest protein purification methods? - (reply: 2)
-
Tandem affinity purification (TAP) mystery - from euphoria to bummed out (reply: 2)
-
Please recommend a fast way to get purified protein - (reply: 2)
-
Huge DNA loss after purification - (reply: 8)
-
Plasmid DNA purification - (reply: 3)
-
DNase I......how much to use in units? - protein purification issues (reply: 1)
-
protein purification - (reply: 1)
-
Membrane protein purification - (reply: 1)
-
Purify integrating PCR product before transforming yeast? - (reply: 1)
-
gel purification necessary for ligation? - (reply: 3)
-
Concentrating recombinent antagens - hisTaged antagens purified and neen to concentrated (reply: 1)
-
purification of DNA extracted from agarose gel - does ethidium bromide remains / interefrence with ligation (reply: 1)
-
purification columns for ssDNA - (reply: 1)
-
Protein purification from E Coli - (reply: 11)
-
synaptosome purification - (reply: 1)
-
opinion - denatured protein purification (reply: 1)
-
Gel shift assay for non purified protein preps - (reply: 1)
-
Phenol purification of genomic DNA degraded by RNA? - (reply: 4)
-
Size exclusion spin columns for oligo purification ... - (reply: 1)
-
ridiculous problem - protein purification (reply: 2)
-
Plasmid purification - (reply: 5)
-
Protein purification - (reply: 3)
-
phenol/chloroform of purified PCR product after RE digestion - what are we trying to purify? (reply: 4)
-
Imidazole in column elution - Removing Imadazole from purified protein without dialysis (reply: 1)
-
Easy protocol for purification of in vitro transcribed RNA oligonucleotides? - (reply: 2)
-
gel purification of the vector... - is it good enough for ligation? (reply: 7)
-
purification of prot interacting with a probe in EMSA - (reply: 8)
-
How to purify PCR product? - (reply: 3)
-
Type I collagen - extraction and purification - (reply: 1)
-
purification of digested plasmids - (reply: 4)
-
Porblem using streptavidin coated beads - Using streptavidin coated beads to purify proteins tagged with a biotinylated ol (reply: 2)
-
Spin column for purification of oligonucleotides - (reply: 2)
-
Role of buffer in antibody purification - (reply: 3)
-
protein loss during nickle clolumn purification - is there always a lot loss of protein during column purification? (reply: 2)
-
magic? - got more product after PCR purification... (reply: 3)
-
what is the role of sodium azide in binding buffer - antibody purification (reply: 15)
-
purification for GST pull down - (reply: 1)
-
problem purifying a his-tag protein - (reply: 2)
-
Contamination after purification - (reply: 1)
-
why get rid of imidazole in his tag protein purification - (reply: 3)
-
PCR product purification - (reply: 3)
-
to purify these PCR products or not? - preparation for restriction digest (reply: 5)
-
lysis is not translucent after urea treatment - need help with His tag fusion protein purification (reply: 2)
-
DNA purification from agarose gels - (reply: 2)
-
DNA Purification from gels - (reply: 4)
-
6 His tagged protein purification - (reply: 1)
-
Removing salt and immidazole from purified Protein - (reply: 2)
-
DNA purification for ChIP - way to get clean DNA without using phenol (reply: 4)
-
Ni-NTA column purification - Ni-NTA column purification (reply: 1)
-
His-tag protein purification - (reply: 1)
-
why glycerol used in protein purification - (reply: 1)
-
imidazole in protein purification - (reply: 2)
-
gel purification after restriction digestion - (reply: 3)
-
purification of long single stranded DNA - (reply: 4)
-
Ab purification from supernatant - dialysis of large volumes (2L) (reply: 3)
-
Problem in purification of a bacterial transcription factor - Help...please....I only have 5 months left (reply: 4)
-
Best way to purify PCR product for sequencing - (reply: 2)
-
Need help purifying DNA oligoes - (reply: 2)
-
anti-c-myc-agarose protein purification - (reply: 1)
-
antibody purification - (reply: 5)
-
Can we purify Miniprep DNA and use for transfection? - (reply: 3)
-
conservation of antibodies! - after purification! (reply: 5)
-
Aggregation of protein after purification? - (reply: 2)
-
WBC RNA preservation and purification (NEED HELP!) - (reply: 3)
-
purify Ab precipitate after neutralize to pH7 - (reply: 2)
-
purification by gel? - after a first round of PCR (reply: 2)
-
DNA purification - (reply: 1)
-
Appearence of non specific[non 6His tagged] proteins with Ni-NTA purification. - (reply: 16)
-
Purification of enzymatically active protein from PAGE - (reply: 9)
-
How to purify and clone a 88 bp fragment? - (reply: 4)
-
SOS: Gel purification of small size DNA - (reply: 6)
-
Purify genomic from plasmid DNA - (reply: 5)
-
purification of Anti-p24 antibody - purification of Anti-p24 antibody (reply: 1)
-
Question about gel purification of PCR product - (reply: 1)
-
protein purification - (reply: 1)
-
protein purification - (reply: 3)
-
Help with purification of antibody against a GST-fusion - Antibody purification (reply: 2)
-
Purifying a membrane protein - - the trouble with lipids? (reply: 3)
-
Genomic DNA purification from few Tissue Cultured cells - (reply: 2)
-
Concentration limit for centricon filters - protein purification (reply: 2)
-
High quality gDNA - need recommendation for purification method (reply: 1)
-
Purification of proteins from human tissue - SDS_PAGE (reply: 1)
-
Is it possible to gel purify the ligated product? - Avoid non cut vector or self-liagted vector contam (reply: 2)
-
nonspecific band after purification - (reply: 2)
-
Odd curve of Gel purified PCR product - (reply: 5)
-
gel purification - (reply: 1)
-
PCR purification basics? - (reply: 6)
-
Purification of short PCR product - (reply: 4)
-
Large scale purification of large size plasmid - (reply: 1)
-
Housekeeping Gene Selection / RNA purification troubles - Molecular Biology (reply: 2)
-
RNA purification from macrophages - (reply: 5)
-
Imidazole reduction causes precipitation - protein purification (reply: 2)
-
Oligo purification - (reply: 5)
-
Purification of small DNA molecules - (reply: 6)
-
Proteins and marker are not running down gel - Protein purification (reply: 7)
-
Cesium Chloride Density purification before IP - (reply: 1)
-
Does CHAPS interfere with GST purification?? - (reply: 1)
-
About pH and pI for his-tagged protein purification - (reply: 2)
-
Purification after digestion - do I need to do it? (reply: 1)
-
His-tag degraded after purification? - (reply: 3)
-
dialysis of purified protein - (reply: 5)
-
Alternative to phenol-chloroform purification of DNA - Genomic DNA purification (reply: 4)
-
Using qiagen kit for ChIP DNA purification - (reply: 4)
-
Trizol followed by column purification - (reply: 9)
-
can i autoclave buffers with imidazole for His-tag purification? - (reply: 2)
-
His-taged protein is in 8M Urea, how to get rid of urea? - Ni-NTA column purified protein in 8M urea buffer (reply: 3)
-
Purification of Genomic DNA - (reply: 2)
-
Insect cell sonication protocol? - Protein Purification (reply: 1)
-
Anybody happens to know how to purify RubisCO - (reply: 1)
-
his tag - his tag purification (reply: 2)
-
Unspecific bands after gel purification, why? - (reply: 7)
-
Help for purification - (reply: 3)
-
batch purification - (reply: 1)
-
Quick way of purifying DNA? - Preparative Gel is time consuming (reply: 3)
-
His-Tag purification using Qiagen Ni-NTA spin column - Extra band of unknown protein beside target protei (reply: 2)
-
Purification of small (100bp) PCR products - (reply: 7)
-
problems purifying plasmid DNA after restriction digestion - (reply: 3)
-
DNA purification kit for Microinjection - Anyone trying to purify fragements around 45 kb? (reply: 1)
-
Plant cell wall protein - remove the polysaccharide and purify the protein (reply: 1)
-
protein precipitation after IMAC - protein purification (reply: 1)
-
transfection-grade plasmid - plasmid extraction or purification kit for transfection (reply: 4)
-
Purification through the Ni-NTa column - (reply: 1)
-
Purification of Double stranded radiolabelled DNA - Purification problems (reply: 3)
-
Reuse of PCR purification column - (reply: 1)
-
separated two protein - protein purification (reply: 4)
-
Removal of endotoxin from proteins & DNA - Protein & DNA purification (reply: 15)
-
Promega Gel Purification Kit - Cant get rid of ethanol (reply: 3)
-
aggregation of purified proteins - (reply: 4)
-
A Question about purification in making clones - (reply: 2)
-
purify linearized plasmid for transfection - (reply: 1)
-
Taq polymerase - Produce and Purify (reply: 3)
-
His-tag protein purification - contamination with other... - ...His-rich proteins (reply: 15)
-
Anybody knows any resolutions to improve TAP purification? - (reply: 1)
-
Problem with his tag protein purification - (reply: 2)
-
DNA purification - (reply: 3)
-
protein purification - his-tag protein purification (reply: 2)
-
help for large-scale plasmid purification - (reply: 3)
-
digestion & purification problem - (reply: 6)
-
his tag purification - (reply: 1)
-
protein purification problem - DNA stuck to protein problem (reply: 1)
-
TAP Tagged Affinity Purification + Sticking to IgG-seph - (reply: 5)
-
clonning gel purification - (reply: 1)
-
purification of RNAP from E.coli - (reply: 2)
-
How can I purify these nonautoclavables? And do I need to? - (reply: 2)
-
problems with loss of purified protein due to precipitation - protein purification (reply: 4)
-
Plasmid DNA purification - DNA purification (reply: 7)
-
Purification of nuclear proteins - (reply: 2)
-
His tag protein purification problem - (reply: 66)
-
DNase treatment for RNA purification - (reply: 6)
-
Peptide Purification - (reply: 3)
-
PCR product purification - (reply: 58)
-
Antibodies - Problem in raising and purifying Ab (reply: 1)
-
Problems purifying DGGE bands - PCR/DGGE (reply: 8)
-
problem by using percoll to purify primary cell - (reply: 1)
-
His purification of a regular expressed protein - (reply: 1)
-
Virus DNA - Where can i find purified virus DNA (reply: 2)
-
Purification of a cell membrane protein - (reply: 1)
-
His-tag purification and his-antibody for western - (reply: 2)
-
protein precipitates after dialysis - Protein purification (reply: 4)
-
Murine MAb IgG purification - monoclonal antibody purification from ascites & supernatant (reply: 1)
-
IgM Purification - (reply: 1)
-
Ligation in PTYB12 vectors ofthe IMPACT protein purification system... - (reply: 1)
-
His-tag protein purification - (reply: 5)
-
his-tagged protein purification - (reply: 1)
-
Ni affinity purification - not as simple as you may think - Protein purification (reply: 5)
-
Need to purify total RNA sample(contaminated with DNA) - (reply: 4)
-
antigen purification - (reply: 1)
-
RNA probe purification - (reply: 4)
-
His tag - protein purification - (reply: 2)
-
purifying supercoiled DNA? - trying to separate supercoiled from nicked (reply: 1)
-
Fibronectin Purification - (reply: 1)
-
protein purification - (reply: 3)
-
how to purify small fragment of DNA? - (reply: 2)
-
phantom band in DNA purification - A new band appears after purifying DNA from agarose gels (reply: 1)
-
Problems with PCR Purification - When I try to purify my PCR product I lose all of my DNA? (reply: 4)
-
primer purification - search method for primer purifying (reply: 3)