purification of catalase from liver? - (Oct/19/2006 )
can anyone help me to find a protocol for the purification of catalase from rabbit liver???????and i will be greateful........... 
-ya7an-
Since I didnt have the time and materials to purify a liver enzyme.. I used whole liver extracts in my assays with good results
QUOTE (ya7an @ Oct 19 2006, 09:00 PM)
can anyone help me to find a protocol for the purification of catalase from rabbit liver???????and i will be greateful........... -tertu-
[Since I didnt have the time and materials to purify a liver enzyme.. I used whole liver extracts in my assays with good results]
u are right, the assay gives a good results when u use the whole liver extracts, but the thing that i need is to get the enzyme in a purifyed form, so i need help to get there. 
-ya7an-
QUOTE (ya7an @ Oct 20 2006, 12:21 PM)
[Since I didnt have the time and materials to purify a liver enzyme.. I used whole liver extracts in my assays with good results]
u are right, the assay gives a good results when u use the whole liver extracts, but the thing that i need is to get the enzyme in a purifyed form, so i need help to get there.
u are right, the assay gives a good results when u use the whole liver extracts, but the thing that i need is to get the enzyme in a purifyed form, so i need help to get there.
I bet you can find it from one of the series "Methods in Enzymology".
-genehunter-1-
QUOTE (genehunter-1 @ Oct 22 2006, 01:15 AM)
QUOTE (ya7an @ Oct 20 2006, 12:21 PM)
[Since I didnt have the time and materials to purify a liver enzyme.. I used whole liver extracts in my assays with good results]
u are right, the assay gives a good results when u use the whole liver extracts, but the thing that i need is to get the enzyme in a purifyed form, so i need help to get there.
I bet you can find it from one of the series "Methods in Enzymology".
u had won the bet, but its my bad luck that the procedure in "Methods in Enzymology" needs larg amount of beef liver ( more than 100g) and i have rabbit liver of about 40g also - the method was for large scale- so i stell need help. 
-ya7an-
QUOTE (ya7an @ Oct 22 2006, 12:23 PM)
QUOTE (genehunter-1 @ Oct 22 2006, 01:15 AM)
QUOTE (ya7an @ Oct 20 2006, 12:21 PM)
[Since I didnt have the time and materials to purify a liver enzyme.. I used whole liver extracts in my assays with good results]
u are right, the assay gives a good results when u use the whole liver extracts, but the thing that i need is to get the enzyme in a purifyed form, so i need help to get there.
I bet you can find it from one of the series "Methods in Enzymology".
u had won the bet, but its my bad luck that the procedure in "Methods in Enzymology" needs larg amount of beef liver ( more than 100g) and i have rabbit liver of about 40g also - the method was for large scale- so i stell need help. check your PM
-genehunter-1-