GST protein purification - (Dec/14/2008 )
Hi,
I wander if you could answer my question. If i live the bacteria pellet which express the GST protein in -20 degree refrigerater over night, what will happen? Will the protein degrade after i thaw them to purify?
Thank you! Your replies are appreciate.
yiruz
-yiruz-
QUOTE (yiruz @ Dec 14 2008, 08:46 PM)
Hi,
I wander if you could answer my question. If i live the bacteria pellet which express the GST protein in -20 degree refrigerater over night, what will happen? Will the protein degrade after i thaw them to purify?
Thank you! Your replies are appreciate.
yiruz
I wander if you could answer my question. If i live the bacteria pellet which express the GST protein in -20 degree refrigerater over night, what will happen? Will the protein degrade after i thaw them to purify?
Thank you! Your replies are appreciate.
yiruz
In most cases your protein should be fine. I usually store the pellets at -80 though.
-smu2-