How to purify biotin labled DNA from mix - (Jun/26/2008 )
Hi everybody, Now I have PCR product mix from one primer labled with biotin, one primer labled nothing. So how can I purify biotin labled DNA from mix? Thanks a lot!
-IPS-
You can order Quick Spin Columns for biotinylated DNA purification; Sephadex G-50 (Roche Applied Bioscience).
-larryking-
QUOTE (larryking @ Jun 26 2008, 02:08 PM)
You can order Quick Spin Columns for biotinylated DNA purification; Sephadex G-50 (Roche Applied Bioscience).
Thanks for your explain. But I found a direction in the instruction "Exclusion limit: <72 base pairs" , the DNA I want to exclude is 1000bp, Can I use it ?
-IPS-