protein purification - (Apr/21/2007 )

Hi can ayone tell me an alternative name for ammonia sulphate precipitation ?

and can anyone tell me two solubility techniques other then dialysis?

Thank you

-martyn02-

QUOTE (martyn02 @ Apr 21 2007, 05:52 PM)

Hi can ayone tell me an alternative name for ammonia sulphate precipitation ?

and can anyone tell me two solubility techniques other then dialysis?

Thank you

- salting out
- solvent extraction and ???

-ms-olli-

QUOTE (ms-olli @ Apr 21 2007, 05:23 PM)

- solvent extraction and ???

diafiltration (?)

-mdfenko-

QUOTE (martyn02 @ Apr 21 2007, 10:52 AM)

Hi can ayone tell me an alternative name for ammonia sulphate precipitation ?

and can anyone tell me two solubility techniques other then dialysis?

Thank you

solubility techniques other then dialysis?
- Centrifugation technique

-Mikevnjp-

Hi

I think, salting out???

-Ritachha-

QUOTE (martyn02 @ Apr 21 2007, 07:52 PM)

Hi can ayone tell me an alternative name for ammonia sulphate precipitation ?

and can anyone tell me two solubility techniques other then dialysis?

Thank you

Q1: non-denaturing precipitation/dehydration

Q2: (dialysis as a solubility technique???)

using appropriate solvents
sonification
warming up
permanent shaking

-The Bearer-