problem in protein purification phenol by phenol extraction - (Oct/24/2006 )

I tried phenol extraction method for protein purification... but i am not getting a aqueous layer as supernatant as expected... but get a white fluffy pellet... so.. and a viscous green supernatant (Cyanobacterial culture)...

i couldn't guess were my proteins would be...

need some help in this regards...

cheerios...

pradeep

-PradeepKumarLogeswaran-

QUOTE (PradeepKumarLogeswaran @ Oct 24 2006, 09:01 PM)

I guess the supernatant is the water phase; the lower phase of phenol appears to contain some precipitates, I guess of protein; I would think of an alternative protocol, there seems to be some serious problems in your current protocol

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-The Bearer-