Protocol Online logo
Top : Forum Archives: : Protein and Proteomics

GST and His tagged proteins - Does the GST give problems in His tag purification? (Mar/21/2007 )

My proteins are GST and His tagged - can I just make a His tag purification on these?
Thanks!

-lenenoerby-

One protein with both fusion protein? or combination of both protein with GST and protein with HisTagged?

happy.gif

-timjim-

QUOTE (timjim @ Mar 22 2007, 03:56 AM)
One protein with both fusion protein? or combination of both protein with GST and protein with HisTagged?

happy.gif



I have one protein with both GST AND His

smile.gif

-lenenoerby-

QUOTE (lenenoerby @ Mar 21 2007, 07:25 AM)
My proteins are GST and His tagged - can I just make a His tag purification on these?
Thanks!

of course you can.

-mdfenko-

I have just purified a protein using the pet42a vector from Novagen. This contains both GST and His is easily purified using Ni-NTA beads.

smile.gif

-Aptazoom-

one should be able to purify using nickel columns.

-scolix-