GST and His tagged proteins - Does the GST give problems in His tag purification? (Mar/21/2007 )
My proteins are GST and His tagged - can I just make a His tag purification on these?
Thanks!
-lenenoerby-
One protein with both fusion protein? or combination of both protein with GST and protein with HisTagged?
-timjim-
QUOTE (timjim @ Mar 22 2007, 03:56 AM)
One protein with both fusion protein? or combination of both protein with GST and protein with HisTagged?
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I have one protein with both GST AND His
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-lenenoerby-
QUOTE (lenenoerby @ Mar 21 2007, 07:25 AM)
My proteins are GST and His tagged - can I just make a His tag purification on these?
Thanks!
Thanks!
of course you can.
-mdfenko-
I have just purified a protein using the pet42a vector from Novagen. This contains both GST and His is easily purified using Ni-NTA beads.
-Aptazoom-
one should be able to purify using nickel columns.
-scolix-