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Expression Related Discussions
  1. No expression seen on PAGE - Shall I still go for costly protein-activity assay? (reply: 6)
  2. cloning into bacterial expression vector - problem in E.coli ttransformation (reply: 4)
  3. Expression of protein in non target strain but unfortunately no protein expressi - (reply: 1)
  4. how to confirm expression - (reply: 2)
  5. boosting expression in stable cell line? - (reply: 5)
  6. Compare the expression of two target genes - (reply: 3)
  7. no expression in pET 21 vector - (reply: 2)
  8. problem in protein expression after 8 months - (reply: 2)
  9. mysterious puzzle in result of confirmatin the protein expression after 3 years - (reply: 2)
  10. NO expression in pKK223-3 vector in DH10B Why ? - in DH10B NO expression in pKK223-3 vector in DH10B Why ? (reply: 2)
  11. Exogenous protein expression - Problems in protein expression mammalian cells (reply: 2)
  12. Mammalian expression problem - HEK cells expression (reply: 3)
  13. pls recommend a serum independent expression promoter for mammalian cells - (reply: 1)
  14. miRNA expression vector - (reply: 3)
  15. expanded band of the purified protein on silverstain gel (even in low expression - (reply: 6)
  16. no expression of my target protein - (reply: 3)
  17. expression level comparison - a database to find gene expressi (reply: 2)
  18. Gene Expression level of apoptotic-related genes by qReal Time RT PCR - (reply: 1)
  19. "Gene expression vs Transcription vs mRNA levels": Help with conceptua - (reply: 1)
  20. mRNA levels, transcription or Gene expression? - (reply: 2)
  21. Technique to estimate the level of protein expression from media - (reply: 2)
  22. unexpected 40kDa band -E.coli protein expression - (reply: 3)
  23. DeltaDelta Ct Relative or Comparative Quantitation - methods of relative quantitation for qPCR determining gene expression (reply: 3)
  24. statistics - gene expression quantification (reply: 1)
  25. GST-tagged protein expression problem-plz help - (reply: 3)
  26. Troubles with expression proteins - (reply: 7)
  27. can shRNA expression set share one polyA terminator with other gene - (reply: 3)
  28. A problem with transient expression a myc-tagged protein in 293T cells - (reply: 1)
  29. mRNA Expression - How to observe mRNA expression of gene. (reply: 10)
  30. cytosolic b-catenin expression - (reply: 2)
  31. how to optimize rt-pcr for gene expression analysis - (reply: 3)
  32. Normal PCR for looking expression - (reply: 1)
  33. how to improve protein expression - (reply: 2)
  34. gene expression analysis - (reply: 1)
  35. Comparing surface protein expression of different cell lines - (reply: 1)
  36. what cells to use for expression of pGEX3? - (reply: 1)
  37. Analysis protein expression - (reply: 1)
  38. Bacterial expression antibody recognition problem - antibodies won't recognize protein (reply: 2)
  39. Expression vector and Stop codon consideration - (reply: 2)
  40. How to construct miRNA expression vector - (reply: 1)
  41. Protein expression using tap water? - (reply: 2)
  42. Protein expression problems in HB101 - (reply: 1)
  43. E. coli MC4100 and T7 expression system - (reply: 3)
  44. protein expression problem - (reply: 1)
  45. how to select a calibrator for relative gene expression in real-time PCR - also about the inconsistant about the triplicate of real-time PCR (reply: 6)
  46. proteins expression - (reply: 1)
  47. Transient Transfection into HEK -> EL expression - (reply: 2)
  48. Baculovirus expression problem - Mixture of Bacmid DNA (reply: 2)
  49. Recombinat Insoluble Protein Expression in E.coli - Inclusion body foramtion (reply: 1)
  50. when to set up a RT-PCR for mRNA expression? - detect transcription of transgene (reply: 1)
  51. checking mRNA expression by reverse transcriptase PCR - (reply: 2)
  52. mRNA expression in T-DNA insertion mutant - Arabidopsis (reply: 1)
  53. Expression of EGFR in MDA-MB-435 cell line - (reply: 2)
  54. measuring expression of TNF-a in macrophages - (reply: 1)
  55. Can we measure gene expression with standard PCR? - (reply: 1)
  56. How do I make a graph displaying "# of Genes" vs. "Expression Lev - (reply: 4)
  57. protein expression in E.coli - no expression after induction (reply: 1)
  58. expression by hsp60 in E.coli - (reply: 3)
  59. expression by hsp60 in E.coli - expressions (reply: 1)
  60. How to induce expression in mammalian cells with the pcDNA/V5/GW vector ? - (reply: 3)
  61. pichia expression - (reply: 2)
  62. Lack of Expression in pQE30 clone - (reply: 8)
  63. TAT-GFP expression from pRSET vector - (reply: 1)
  64. Expression Vector sequencing problem - (reply: 6)
  65. Expression Vector sequencing problem - (reply: 1)
  66. Expression of GFP and DsRed tagged proteins - co-localization needed for FRET (reply: 9)
  67. Checking mRNA expression by RT-PCR - (reply: 2)
  68. primer design - over expression (reply: 1)
  69. Extracellular protein expression in E.coli - anyone knows a good commercial vector? (reply: 1)
  70. Using IRES in microRNA expression vector - (reply: 4)
  71. RNA in expression array - (reply: 1)
  72. more quesitons - hetologous expression systems (reply: 4)
  73. alphaTC1-9 expression after 1 month - (reply: 2)
  74. cannot find the expression protein - (reply: 5)
  75. How can I increase protein expression in S. cerevisiae? - (reply: 6)
  76. How to increase expression of protein in yeast? - Western from yeast and liquid beta-galactosidase assay (reply: 1)
  77. primer conditions for expression work - (reply: 5)
  78. Inducible Promoters/Repressors for Tissue-Specific Expression? - (reply: 3)
  79. basic questions regarding expression vector work - (reply: 2)
  80. Leaky Expression? - (reply: 5)
  81. Interpreting PCA Graph for Gene Expression - Principle Component Analysis in Gene Expression (reply: 1)
  82. unstable protein expression,pleeeeeese help - (reply: 6)
  83. Why a previously soluble protein is now going into inclusion bodies? - protein expression (reply: 4)
  84. Is it total ORF is required for complete gene expression - (reply: 3)
  85. Western blot is not working with stable cell line=please help - no expression of my protein detected (reply: 5)
  86. western blot problem - no expression on stable transfection. (reply: 9)
  87. how to calculate expression when control dont express - (reply: 3)
  88. What Ct value is accepted as expression? - qRT-PCR Ct values (reply: 3)
  89. which part of gene is needed for normal expression in vector - (reply: 2)
  90. how to check organ(tissue)-specific expression profile of a gene - (reply: 3)
  91. housekeeping gene for comparing gene expression between tumor and normal tissue - (reply: 1)
  92. help with luciefarse reporter assay,:( - p53 reporter vector versus expression plasmid (reply: 1)
  93. Protein expression changes after freezing+thawing - (reply: 1)
  94. Internal control for RT-PCR analysis - The expression of internal control (reply: 3)
  95. No protein expression in pKK223-3 vector in JM105 - (reply: 3)
  96. Surface expression - (reply: 2)
  97. GST expression problem - low fusion, high GST alone (reply: 3)
  98. expression problem - expression (reply: 1)
  99. ABCG2 expression and MCF7 - (reply: 1)
  100. Expression problem - (reply: 6)
  101. RNAi rescue by ectopic expression - (reply: 2)
  102. Confirmation of protein expression in pCDNA3 - How to know foreign gene expression in pCDNA3 (reply: 8)
  103. Protein expression in S.cerevisiae - (reply: 4)
  104. Expression vector for Pseudomonas - expression of a 10Kb gene in Pseudomonas aeruginosa (reply: 2)
  105. gene from 4-TOPO to expression vector - (reply: 1)
  106. help!real-time PCR to see gene's expression after treatment with cytokin - (reply: 4)
  107. Problem with expression of DsRed fusion protein. - (reply: 4)
  108. Alpha-Amylase expression and purification - (reply: 3)
  109. problem in expression of target protein - (reply: 11)
  110. concentrating low expression protein for western - will this work? (reply: 3)
  111. Protein expression in Gram(-) bacteria - Protein expression by using special S-D sequence (reply: 1)
  112. What is the best protein expression system - (reply: 1)
  113. expression of a two subunits receptor - (reply: 1)
  114. Replacing EGFP with RFP..but no expression! - (reply: 10)
  115. Questions about gene expression, codons, and Kozak sequence - (reply: 4)
  116. protein expression in NSO cells - (reply: 6)
  117. NO EXPRESSION IN LARGE SCALE - (reply: 2)
  118. how to determine the expression level of 2 different transgenes - (reply: 1)
  119. Protein expression in E.coli DH5alpha? - (reply: 1)
  120. should I cut out signal peptide for expression - (reply: 1)
  121. Protein expression help needed - Mammalian cells (reply: 11)
  122. inductions - inductions for expression of protiens (reply: 1)
  123. Probelm with expression of ~85 kDa GSt fusion protein. - (reply: 5)
  124. basics about expression vectors - (reply: 2)
  125. Suggest a name of a bidirectional expression vector - Anyone has idea of this vector? (reply: 1)
  126. How to know tissue specific gene expression by real time PCR - (reply: 3)
  127. Prtocol for large scale protein expression - (reply: 1)
  128. losing protein expression after stablizing cells( please help) - (reply: 4)
  129. comparing difference in gene expression - (reply: 2)
  130. cytokines anf growth factor expression in bone marrow mesenchymal stem cells - Stem cells (reply: 3)
  131. Protein Expression problem - N-End Rule?? (reply: 1)
  132. No expression from injected GFP&RFP mRNA - (reply: 16)
  133. no protein expression - (reply: 2)
  134. Protein Expression Problem - N-end rule?? (reply: 2)
  135. quantifying my gene expression in 5 different cell lines - (reply: 1)
  136. please recommend some common miRNA expression vectors - (reply: 2)
  137. GST fusion protein expression problem - (reply: 1)
  138. Expression problem in E.coli system using pETT22b+ from Novagen - low level of expression with unknown reason (reply: 4)
  139. Drosophila: Gene knockout using a transposon technology - Why do you get a gene expression? Other methods to knockout a gene? (reply: 7)
  140. siRNA knock down did not work - protein expression knock down by siRNA (reply: 4)
  141. comparison of mRNA expression - (reply: 1)
  142. Scramble siRNA shows increase in gene expression by Taqman RTPCR - (reply: 1)
  143. Gene expression - (reply: 1)
  144. protein expression level of stable transfectant will change? - (reply: 1)
  145. cloning in expression vector - (reply: 5)
  146. PPARs expression in cardiac cells - (reply: 4)
  147. How to chose vector and E.coli strain for protein expression - (reply: 2)
  148. stable transfection high expression - problems with low expression in stably transfected cells (reply: 1)
  149. Eukaryotic recombinant protein expression - (reply: 3)
  150. Loss of expression of EGFP-actin from MCF-7 transfected with pEGFP-actin - (reply: 3)
  151. Protein expression vs. protein activity? - (reply: 2)
  152. Protein expression - (reply: 1)
  153. Stable cell line losing expression faster than usual - (reply: 6)
  154. DsRed-MIP construct : no expression of DsRed - (reply: 7)
  155. Tissue expression database? - Looking for a web page.. (reply: 1)
  156. SOS for GST-fusion protein expression - (reply: 1)
  157. Expression in BL21-DE3 and Rosetta - (reply: 17)
  158. Transcription factors regulation - testing the effect of NFkB on expression (reply: 2)
  159. help finding eukaryote expression vector - (reply: 2)
  160. How do i optimize a shake flask culture conditions for maximum expression of rec - (reply: 1)
  161. Protein expression from bacteria which has two plasmids? - Incompatibility of plasmids (reply: 5)
  162. Which vector would you choose for transient expression in CHO cells - (reply: 2)
  163. how to compare 2 different gene expression level in the same sample? - can I use ratio(A/B)=2^(CtB-CtA)to calculate these two genes' expr (reply: 5)
  164. Gene Expression Omnibus - (reply: 2)
  165. miRNA expression vector - miRNA expression vector (reply: 4)
  166. How to association the methylation profiling with the expression? - statistic questions about the bisulfite sequencing (reply: 1)
  167. peptide expression vector - I need to find a vector for bacterial expression of peptides of varyin (reply: 4)
  168. IRES sequence. Different gene's expression. - (reply: 2)
  169. Bacterial housekeeping genes - What genes are commonly used as housekeeping controls in expression st (reply: 1)
  170. Protein Expression Problem - (reply: 5)
  171. How to measure protein expression levels of multiple forms of glycosylated prote - (reply: 2)
  172. Problems with transgene expression using pcDNA3 - (reply: 5)
  173. NMDA receptor expression, unable to resolve receptor subunits - (reply: 3)
  174. expression problem! - (reply: 6)
  175. pET IPTG expression - Getting protein expression (reply: 1)
  176. Protein expression with IPTG - (reply: 6)
  177. Sudden loss of protein expression in E coli - (reply: 3)
  178. does low mRNA expression mean low relevant protein expression - looking for answers or references (reply: 9)
  179. expression of short RNAs - (reply: 2)
  180. whole gene synthesis for expression - (reply: 3)
  181. Help with Producing lentivirus in 293FT cells - Using Invitrogen's BLOCK-iT Lentiviral ol II miR RNAi Expression S (reply: 1)
  182. confirming equal expression of transcripts in three samples...how to? - (reply: 3)
  183. mutant protein expression & purification problem in E.coli - Protein with five residues mutated to Alanine's (reply: 6)
  184. Protein expression. Question of V5 epitope and 6x His tag ! - (reply: 2)
  185. soluble protein, low expression - (reply: 6)
  186. low to no gene expression - (reply: 4)
  187. Protein expression Using NEB yest kit - (reply: 1)
  188. eukaryotic expression - (reply: 3)
  189. mRNA expression too low? - (reply: 3)
  190. mRNA expression low/no effect of the siRNA - (reply: 1)
  191. Determining low product cut off using Ct - How to decide Ct level at which expression is unreliable (reply: 1)
  192. Dicer knockdown to show miRNA mediated target mRNA expression? - (reply: 6)
  193. Transfection of expression vector for gel shift - how much vector should be transfected? (reply: 2)
  194. IPTG protein expression in BL21 (or orthers) - how to measure the expression (reply: 1)
  195. Cloning & Expression - Cloning & Expression (reply: 7)
  196. finiding the best promoter for induction od toxic protein - protein expression (reply: 1)
  197. YEAST EXPRESSION - (reply: 1)
  198. Gene body methylation and active gene expression - (reply: 6)
  199. cloning expression problem - (reply: 2)
  200. Good review about protein expression in E.coli - (reply: 1)
  201. Stability of a transgene and its expression - (reply: 3)
  202. Bacterial strains used in protein expression - (reply: 5)
  203. RNA expression calculations - (reply: 1)
  204. pgh19 Xenopus expression vector - Need sequence (reply: 3)
  205. expression with M15 - Any special tips?? (reply: 3)
  206. increased/decreased expression in western blot - (reply: 2)
  207. transgene expression - (reply: 3)
  208. protein expression from glycerol stock - (reply: 2)
  209. gene expression study - between treated and untreated cells (reply: 2)
  210. Expression of an N-terminally tagged golgi protein - (reply: 1)
  211. cDNA for real time PCR - for gene expression study (reply: 1)
  212. expression construct need the addition of STOP Codon? - (reply: 2)
  213. Troubleshooting protein expression - GST tag - (reply: 3)
  214. pcDNA3 expression vector is suitable for TNT in vitro? - (reply: 2)
  215. His-tag protein expression problem - (reply: 4)
  216. calculate relative expression using 3 biological replicates - (reply: 1)
  217. Bl21 plys expression system - protocol (reply: 7)
  218. Expression in codon plus of GST fusion protein - (reply: 3)
  219. GFP to expression - (reply: 3)
  220. 2 PCR product to be inserted into 1 expression vector - (reply: 2)
  221. expression problems in mammalian cells - (reply: 2)
  222. Expression of GFP fusion protein - Improving fluorescence visualization (reply: 2)
  223. Transfection and Expression: getting transcript but no protein expression - (reply: 3)
  224. Strange: mRNA expression goes up but protein goes down - (reply: 2)
  225. HELP! no expression in BL21 - (reply: 7)
  226. Stable vs transient - which gives the highest yield? - I'm setting up a expression system for a secreted protein, should I choose s (reply: 2)
  227. Expression in other mammalian cells with human H1 promoter - (reply: 1)
  228. Help: Tubulin expression is not the same between my cell clones? - (reply: 1)
  229. Transfection by electroporation of 293 suspension cells - electroporation of 293 cells for large scale transient expression (reply: 2)
  230. Real Time PCR and Gene expression - (reply: 1)
  231. CHO culture: what media and additives? - for stable Fc fusion protein expression (reply: 4)
  232. Need Some Math Help - stimulation of gene expression (reply: 3)
  233. miRNA quantification - easiest/cheapest method of measuring expression of a single miRNA in differnt ce (reply: 3)
  234. protein expression in BL21 cells - problems (reply: 7)
  235. expression vectors - expressing inserts in either orientation (reply: 2)
  236. Lentiviral expression of a secreted protein - Any suggestions where I am going wrong? (reply: 1)
  237. Protein expression - decreased expression (reply: 2)
  238. Options for protein expression? - (reply: 3)
  239. How to normalize the protein expression amongst different tissues - (reply: 7)
  240. Maltose Binding Fusion Protein MBP - which strain for expression? (reply: 1)
  241. Cloning and Expression vectors - Which is the best Expression vector to use? (reply: 3)
  242. Testing expression of OVA gene - (reply: 1)
  243. vector for expression of tag on cell surface - (reply: 7)
  244. baculovirus expression system - (reply: 3)
  245. FLAG-Tag Protein expression in MCF-7 - (reply: 8)
  246. protein expression in pET-30a vectors - (reply: 2)
  247. Relative expression of genes between bacterial species - (reply: 1)
  248. who can recommend me a good baculovirus expression system? - (reply: 3)
  249. need help in facs data analysis of surface expression - (reply: 3)
  250. Gene expression on same Chromosome region possible? - microarray (reply: 3)
  251. who can compare adenoviral expression system and baculoviral expression system - (reply: 2)
  252. far western - Would the expression of the protein in mammalian cells be enough for far wester (reply: 2)
  253. to check protein expression - (reply: 4)
  254. Question on adenovirus expression - (reply: 2)
  255. AT1 receptor expression and quantification (WB) - need advice (reply: 1)
  256. low expression efficiency using 293T to produce retrovirus - does Na pyruvate matter for 293T growth ? (reply: 6)
  257. Verify the protein spots found by 2D using Western blot - The expression level change are different (reply: 2)
  258. problem of protein expression - problem of protein expression (reply: 1)
  259. qPCR Analysis - Standard Error of Individual dCT vs Group average expression (reply: 4)
  260. Protein expression troubleshooting - problem in expression of my protein (reply: 3)
  261. Help for transient expression in HEK-293 - (reply: 5)
  262. Thrombin - pDEST20/GST-Baculovirus - Cloning and Protein expression (reply: 3)
  263. strange RT-PCR expression - (reply: 1)
  264. change in expression of House keeping gene - change in expression of House keeping gene (reply: 2)
  265. Cloning WITHOUT Topoisomerase! - Does any one know of an expression vector that does not depend on Topoisomerase (reply: 13)
  266. Glucose - expression repressor - (reply: 1)
  267. expression of npcRNA - (reply: 2)
  268. Expression of fusion protein in mammalian cells - (reply: 2)
  269. Cloning into expression vector - Why doesn't it work anymore? (reply: 7)
  270. GST + 6kD protein expression? Doesn't seem to be working! - (reply: 9)
  271. Expression of peptide containing alternating d- and l-amino acids - (reply: 13)
  272. peptide expression problem - (reply: 4)
  273. 3'UTR and Gene expression in expression construct - (reply: 2)
  274. how can I increase protein expression level - hurry (reply: 2)
  275. Is myc expressed in hippocampal neurons? - endogenous myc expression in hippocampal neurons? (reply: 4)
  276. gene expression assay - (reply: 1)
  277. co-transfection - co-transfection affect gene expression? (reply: 4)
  278. cDNA synthesis ASAP - gene expression (reply: 3)
  279. tRNA expression and purification - (reply: 1)
  280. Expression of GFP fusion: splicing out of tag ? - (reply: 1)
  281. stable or transient transfection method? - for protein expression (reply: 4)
  282. very low Expression in Bioreactor? - (reply: 3)
  283. Cell line to be used for protein expression an purification - (reply: 8)
  284. What is the best positive expression control for your experiments? - Which is better? (reply: 6)
  285. Protein expression problem - coexpression? (reply: 1)
  286. TLR expression on Vero cells - (reply: 2)
  287. Problems with protein expression - Protein expression, Antibody production (reply: 2)
  288. loss of expression in stable transfection - (reply: 4)
  289. expression in Rosetta pLysS - how to get a good expression level?? (reply: 1)
  290. buffer for expression analysis - (reply: 3)
  291. How to study the expression of one, specific gene? - (reply: 5)
  292. Measuring receptor expression by FACS - interference with trypsin exposure? (reply: 2)
  293. DNA methylation vs gene expression - (reply: 3)
  294. problems with expression of mutant protein - (reply: 1)
  295. What to choose as a calibrator? - When looking at basal gene expression (reply: 7)
  296. basal expression - Is it possible in DH5a or in Rosetta pLYS? - (reply: 3)
  297. Effect of scrambled siRNA on gene expression - (reply: 5)
  298. Expression temprature - (reply: 3)
  299. special band in BL21(DE3) - protein expression (reply: 8)
  300. Expression analysis - (reply: 1)
  301. pEt 28-a expression system - (reply: 2)
  302. No success with PCR cloning of a gene into expression vector - (reply: 5)
  303. expression of a gene - help me (reply: 3)
  304. protein expression - (reply: 3)
  305. About protein expression - (reply: 1)
  306. Problem in protein expression induction - (reply: 7)
  307. How to increase protein expression level in cell lines - (reply: 2)
  308. Expression vector - (reply: 1)
  309. RNAi confusion - More expression! (reply: 7)
  310. FACS analysis of receptor surface expression using fluorescent antibodies - (reply: 3)
  311. Use the Pichia secreted expression system - White ppt formed in the supernatant after stored at -80 C fridge (reply: 4)
  312. Measure OD600 when perform the protein expression - (reply: 6)
  313. Linear or unlinear plasmid before transfection for expression? - (reply: 2)
  314. Effect of radiation in hypoxic and acidic cells - how to initiate the experiment about protein expression in hypoxic and acidic ca (reply: 2)
  315. ask some question about gene expression data - (reply: 1)
  316. To make good plasmid for expression transformation - (reply: 1)
  317. how to increase protein expression in E coli? - (reply: 6)
  318. Basal level Expression - (reply: 1)
  319. IPTG expression w/ GST-fusion gene - (reply: 5)
  320. how to determine tissue specific expression of gene? - (reply: 2)
  321. How to calculate absolute fold change in expression by realtime PCR - Realtime PCR (reply: 2)
  322. good vectors for neuronal expression - (reply: 13)
  323. Expression of N-terminal GFP fusionproteins - Proteins with N-terminal signalpeptide (reply: 3)
  324. Question about protein expression induction - (reply: 2)
  325. Knockdown protein expression using rnai - (reply: 4)
  326. Western Blot Hela Cos7 - Show nearly identical genes of two related organisms same expression (reply: 4)
  327. low-level mammalian expression - (reply: 2)
  328. Uneven dye balance (within slide) - Relative dye expression changes from left to right on slide (reply: 1)
  329. protein expression in mammalian cell - (reply: 1)
  330. Murine Macrophage - Il-12 and Il-10 Expression? - (reply: 4)
  331. gene expression - (reply: 2)
  332. Large protein expression in E.coli - (reply: 5)
  333. How many fold change make sense during aza treatment - gene expression after aza expression (reply: 3)
  334. No expression of his-tagged protein - (reply: 4)
  335. RNA pol II driven shRNA expression - CMV driven shRNA expression (reply: 10)
  336. RNAi in plant and detect with real time PCR - Detect gene expression decrease with qRT-PCR (reply: 2)
  337. DH5 Alpha protein expression - (reply: 1)
  338. Mammalian expression vector - (reply: 3)
  339. Plant expression vector - (reply: 4)
  340. Expression interference from His-tag - transcription protein (reply: 2)
  341. Methylation and expression of imprinted genes - (reply: 3)
  342. pQE30 - protein expression (reply: 1)
  343. Expression problem in pQE30 vector system - (reply: 2)
  344. general question- how to study regulation of gene expression - (reply: 2)
  345. pCIS expression vector - (reply: 2)
  346. trouble in scaling-up protein expression - (reply: 5)
  347. Antibiotic marker expression in e.coli and geobacilli - (reply: 1)
  348. 3.5 kDa peptide expression in periplasm or medium - (reply: 1)
  349. Cytochrome P450 expression - (reply: 11)
  350. expression of transfected gene - urgent (reply: 5)
  351. protein expression ! - please help me with this.. (reply: 1)
  352. No insert for cloning PCR product into expression vector - (reply: 5)
  353. problem in expression of his tag protein - (reply: 6)
  354. bicistronic retroviral vector - Two both gene mammalian expression (reply: 2)
  355. Quantify protein expression - (reply: 1)
  356. expression under fungal promoter in bacteria (?) - (reply: 4)
  357. Kozak Sequences and Mammalian Expression Vectors - (reply: 2)
  358. protein expression - (reply: 3)
  359. nutrients & gene expression - (reply: 2)
  360. Gene Expression Data - Looking for gene expression data (reply: 2)
  361. gene expression vs protein expression - (reply: 3)
  362. Adeno-X Expression System problems - (reply: 3)
  363. codon bias? - protein expression (reply: 11)
  364. Factors that may influence recombinant protein expression - (reply: 9)
  365. Advice on Protein expression Services - (reply: 4)
  366. protein expression protein - molecular biology (reply: 6)
  367. peptide expression - (reply: 3)
  368. Northern vs qRT-PCR for expression quantitation - (reply: 6)
  369. Proetin expression in insect cells - (reply: 1)
  370. transfection of HiFive Cells? - Baculovirus Expression (reply: 1)
  371. Expression Vectors - construction (reply: 4)
  372. how to monitor gene expression over time in culture - GFP method (reply: 1)
  373. Problem with expression of marker gene - (reply: 1)
  374. Problem with expression of marker gene - (reply: 2)
  375. determining protein expression level from a Western!!! - (reply: 6)
  376. Designing siRNA expression vectors - (reply: 2)
  377. Expression problems with pIRES vector from Clontech - EGFP at MCSB site cannot be expressed! (reply: 1)
  378. primary culture - measuring transcription - mRNA expression in primary culture (reply: 1)
  379. High protein expression but low protein activity - MBP-tagged fusion protein inactive (reply: 2)
  380. No protein expression after IPTG induction - No protein expression after IPTG induction (reply: 6)
  381. expression in Pichia - (reply: 1)
  382. small protein expression in yeast - expression of an 8kD his tagged protein in yeast (reply: 1)
  383. Two cloning and expression problems - (reply: 2)
  384. IPTG induction - no expression/induction (reply: 7)
  385. Protein Expression Analysis Question - Protein Expression Analysis (reply: 1)
  386. expression without kozak? - (reply: 1)
  387. protein expression level optimization - general question (reply: 1)
  388. How to selecte gene for epigenetics research? - imprinted gene or gene with expression change ? (reply: 1)
  389. receptor cloning - screening clones for expression of receptors (reply: 2)
  390. insect expression system - protein expression (reply: 2)
  391. Yeast-2-hybrid - select for Leu expression + co-transformants? (reply: 1)
  392. Protien expression - (reply: 2)
  393. Expression Vector Construct - sequence entire insert? (reply: 3)
  394. Interesting gene expression in a stable transfection - (reply: 2)
  395. Inducable mammalian expression vector - (reply: 3)
  396. Gene expression comparison between different species(!) - (reply: 1)
  397. No expression of 7kDa His-tagged protein - why? - Poor protein expression (reply: 9)
  398. no protein expression after induction using pET vector - (reply: 4)
  399. Protease in expression vector - (reply: 3)
  400. Expression even IPTG induction - (reply: 1)
  401. Prokaryotic and Mammalian Expression Vectors - Why do they have to be different? (reply: 2)
  402. Prokaryotic and Mammalian Expression Vectors - Why do they have to be different? (reply: 3)
  403. inducible expression system - (reply: 2)
  404. Temperature for protein expression - (reply: 2)
  405. strange expression model - (reply: 2)
  406. cloning genes with incompatible restriction sites - Protein expression (reply: 2)
  407. Expression screening of stable transfectants? - (reply: 2)
  408. Over expression in cancer and normal cells - cell bio (reply: 3)
  409. Normalizing protein expression under 24hrs ischemia - normalization with housekeeping genes (reply: 5)
  410. expression of fusion protein - how to lysis membrane protein (reply: 2)
  411. improve pritein expression - (reply: 6)
  412. Plant gene expression in bacteria and yeast - (reply: 3)
  413. Expression of N-terminus fusion protein problem - (reply: 1)
  414. semi quantitative PCR - quantification of gene expression (reply: 1)
  415. Genomic integration of siRNA expression cassette - Comparison of generated clones (reply: 3)
  416. Protein produced with brown color? - I have some problem in protein expression (reply: 1)
  417. pGEM-T easy vector for expression - (reply: 6)
  418. mammalian expression vector - (reply: 1)
  419. S2 cell expression - Protein expression (reply: 2)
  420. more questions about protein expression... - (reply: 1)
  421. Gene expression in mammalian cells - (reply: 6)
  422. Antibiotics selection in mammalian expression - (reply: 3)
  423. Gene expression - inducible Gene expression (reply: 1)
  424. Expression cloning system for protease expression - (reply: 2)
  425. expression vector pHM6's map - (reply: 3)
  426. Protein expression problems - (reply: 4)
  427. problem with expression of GST fusion protein - (reply: 1)
  428. Problem in protein expression - (reply: 5)
  429. PCR comparison of gene expression - (reply: 5)
  430. check protein expression from tissue - (reply: 1)
  431. unstable stables - stables lose expression (reply: 1)
  432. Help: baculovirus expression system? Anyone's used it? - Has anyone used this system (reply: 1)
  433. RNAi vector for stable expression - (reply: 4)
  434. expression problems? - (reply: 3)
  435. expression of small peptide - how to identify the small peptide? (reply: 2)
  436. choosing insect cell for high-cystine protein expression - (reply: 1)
  437. active genes and expression problems! - (reply: 3)
  438. Expression of Proteins for western blot - (reply: 1)
  439. Protein expression troubleshooting - (reply: 2)
  440. 293T cells go wild after >50 passages??? - protein expression changed!?? (reply: 1)
  441. DNA arrays for low gene expression ? - (reply: 1)
  442. pichia pastoris expression system - methods in pichia pastoris expression (reply: 2)
  443. protein expression in Pichia pastoris - (reply: 91)
  444. help with protein expression and detection - (reply: 4)
  445. stable transfection with siRNA expression vector - (reply: 6)
  446. How to get expression information from mRNA? - (reply: 2)
  447. Dot blot on bacteria - screen of protein expression (reply: 1)
  448. metal enzyme - gene expression induction time (reply: 2)
  449. CBP expression problem in mammalian cells - (reply: 2)
  450. CBP expression problems in mammalian cells - (reply: 1)
  451. Protein Expression - (reply: 1)
  452. Kozak consensus sequence in expression vector - is substitutable? (reply: 2)
  453. Help! - Method to enhance protein expression! (reply: 1)
  454. Yeast protein extraction - need to change my expression system... (reply: 3)
  455. Protein expression with IPTG in pET3a system - (reply: 3)
  456. protein quantification - protein expression (reply: 1)
  457. Improve expression level in E. coli - How th improve expression of exogenuous gene in E. coli? (reply: 2)
  458. Expression vector/host for plant genomic sequence - (reply: 3)
  459. difficult expression of human gene in E. Coli - (reply: 3)
  460. protein expression - (reply: 5)
  461. software for expression matrix analysis - (reply: 2)