more quesitons - hetologous expression systems (Jun/20/2008 )
Hi there,
Can someone pls help with the following questions? I've written down what I think (altho most of its prob wrong) but any help will be most appreciated!
what are the limitations/advantages of animal bioassays?....is it basically just stuff like accuracy, we used organ baths in our labs and many times out tension was different or stuff like wrong concnetrations via pipetting..and the advantages being things like able to determine the effects various drugs have on the tissue and able to calculate their potency?
Advantages/disadvantages of in vivo animal studies vs. cells?...in vivo studies lets us see what happens to the whole animal (knockout exps) whilst cell just shows what happens to the tissue it was extracted from, the cost of whole animals vs. cells, certain genes may have a different effect in the embry then in adult.
disadvantages of hetologous expression systems? (no idea bout this one)
thx
b_06er
Can someone pls help with the following questions? I've written down what I think (altho most of its prob wrong) but any help will be most appreciated!
what are the limitations/advantages of animal bioassays?....is it basically just stuff like accuracy, we used organ baths in our labs and many times out tension was different or stuff like wrong concnetrations via pipetting..and the advantages being things like able to determine the effects various drugs have on the tissue and able to calculate their potency?
Advantages/disadvantages of in vivo animal studies vs. cells?...in vivo studies lets us see what happens to the whole animal (knockout exps) whilst cell just shows what happens to the tissue it was extracted from, the cost of whole animals vs. cells, certain genes may have a different effect in the embry then in adult.
disadvantages of hetologous expression systems? (no idea bout this one)
thx
b_06er
Any system other than human may respond differently to the human reality. For example, many mice have had terrible things done to them in the name of research into the human immune response to herpes simplex 1, but mice aren't readily susceptible to human HSV1. The same goes for drug reactions.
One problem with heterologous expression systems is the question of post translation al modifications. You could also think about codon usage ,and the effect of a recombinant protein on the host cell's normal machinery.
animal bioassays could create a lot of controversy with associations such as PETA. a similar association came to my university once and tried to steal cows that were used for digestive processes experiments. even though it might not seem to relevant, i think it is something important to consider when designing an experiment with animals, you never know what these radical associations can make, and ruin years of experiments.
cells studies might give you an insight, but in many cases (i believe most of them) the in vivo experiments give completly different results. just one example i'm familiar with, dna methylation patterns in cell cultured plants are completly different than in any other tissue of a live plant.
and about the heterologous expression systems, i think the main disadvantage you could face is the lack of necessary cell machinery of one system in the other.
06r needs to do his or her own homework
At least they have given it a shot... unlike some who post here.