How do I make a graph displaying "# of Genes" vs. "Expression Lev - (Jul/29/2008 )
Hi -
I've done a few microarray experiments in the past, and I love using dChip for my data mining. However, I got a request from my advisor to create a particular type of graph. On the x-axis, she'd like to see a range of normalized expression levels (from 0 to, say, 5000 in 10 unit stretches). And on the y-axis, a tally of the number of genes that were found in each expression level range.
It'd be a global look at each chip, with the peaks towards the lower end, most likely.
The problem - and my question - is what program can spit this out? I'm sure there's a way to do it in Excel, even, but I have no idea how you'd make a tally of genes from the raw values like that.
I haven't checked the finer points of GeneSpring or GeneSifter, but has anybody seen a graph like this with microarray data?
You're describing a histogram. Excel can do them; see here for example. If you don't have the Data Analysis Tool pak installed in Excel, there are other way to do it -- Google "Excel histograms".
Whoops, I was overlooking that term. I'm a moron. I was manually making COUNTIF() descriptors to do it in Excel before I gave up and came here.
Thanks
Hi,
do you know WinStat? It is a tool that is implemented into excel after installation and very easy to use. Additionally, it provides different layouts of histograms and many more statistical applications. A demo version available for 30 days. In my opinion, the histogram "tool" of excel is not very useful, but maybe in the newest versions of excel it is more advantageous.
Best regards
humpback-whale
A bit harsh, don't you think?
We all have brain freezes; sometimes my brain unfreezes when I'm typing a post -- in trying to explain what I'm asking, I explain it to myself...!Anyway, hope it helped, and welcome to the forums!